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Article: Susceptibility of Candida albicans isolates from the oral cavities of HIV-positive patients to histatin-5

TitleSusceptibility of Candida albicans isolates from the oral cavities of HIV-positive patients to histatin-5
Authors
Issue Date2002
PublisherMosby, Inc. The Journal's web site is located at http://www.elsevier.com/locate/prosdent
Citation
Journal Of Prosthetic Dentistry, 2002, v. 88 n. 3, p. 263-267 How to Cite?
AbstractStatement of problem. Oral surfaces, including the denture-fitting surface, may serve as a reservoir for disseminated candidal infections, particularly in immunocompromised hosts such as patients with AIDS. Histatins are a group of small, cationic antifungal peptides present in human saliva. There is limited information on the antifungal activity of peptides against Candida albicans isolates from HIV-positive patients. Purpose. This study investigated the fungicidal effects of histatin-5 against oral isolates of C. albicans from HIV-positive and HIV-negative patients. Material and methods. An isolate of C. albicans from each of 2 HIV-positive patients (both male) and 3 HIV-negative patients (2 male and 1 female) was obtained. American Type Culture Collection 90028 served as a reference strain. All isolates were identified with sugar assimilation tests and the germ tube test. Fungicidal assays were performed on exponential C. albicans cells in the presence or absence of 0.315 to 50 μm of histatin-5. Numerical data were subjected to 1-way analysis of variance and Tukey's multiple range test (P<.05). Results. Histatin-5 (50 μm) killed more than 95% of C. albicans isolates from HIV-negative patients and more than 90% of isolates from the reference strain. The same treatment induced 75.3% and 66.1% loss of viability in C. albicans isolates taken from HIV-positive patients (A1 and A2 cells, respectively). The difference between the fungicidal effects in the HIV-positive and HIV-negative groups was significant. (P<.05). Conclusion. Within the limited population of this study, C. albicans isolates from the oral cavities of HIV-positive patients were less sensitive to histatin-5 than oral isolates from HIV-negative patients. Copyright © 2002 by The Editorial Council of The Journal of Prosthetic Dentistry.
Persistent Identifierhttp://hdl.handle.net/10722/154210
ISSN
2014 Impact Factor: 1.753
2015 SCImago Journal Rankings: 0.507
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNikawa, Hen_US
dc.contributor.authorJin, Cen_US
dc.contributor.authorMakihira, Sen_US
dc.contributor.authorHamada, Ten_US
dc.contributor.authorSamaranayake, LPen_US
dc.date.accessioned2012-08-08T08:23:54Z-
dc.date.available2012-08-08T08:23:54Z-
dc.date.issued2002en_US
dc.identifier.citationJournal Of Prosthetic Dentistry, 2002, v. 88 n. 3, p. 263-267en_US
dc.identifier.issn0022-3913en_US
dc.identifier.urihttp://hdl.handle.net/10722/154210-
dc.description.abstractStatement of problem. Oral surfaces, including the denture-fitting surface, may serve as a reservoir for disseminated candidal infections, particularly in immunocompromised hosts such as patients with AIDS. Histatins are a group of small, cationic antifungal peptides present in human saliva. There is limited information on the antifungal activity of peptides against Candida albicans isolates from HIV-positive patients. Purpose. This study investigated the fungicidal effects of histatin-5 against oral isolates of C. albicans from HIV-positive and HIV-negative patients. Material and methods. An isolate of C. albicans from each of 2 HIV-positive patients (both male) and 3 HIV-negative patients (2 male and 1 female) was obtained. American Type Culture Collection 90028 served as a reference strain. All isolates were identified with sugar assimilation tests and the germ tube test. Fungicidal assays were performed on exponential C. albicans cells in the presence or absence of 0.315 to 50 μm of histatin-5. Numerical data were subjected to 1-way analysis of variance and Tukey's multiple range test (P<.05). Results. Histatin-5 (50 μm) killed more than 95% of C. albicans isolates from HIV-negative patients and more than 90% of isolates from the reference strain. The same treatment induced 75.3% and 66.1% loss of viability in C. albicans isolates taken from HIV-positive patients (A1 and A2 cells, respectively). The difference between the fungicidal effects in the HIV-positive and HIV-negative groups was significant. (P<.05). Conclusion. Within the limited population of this study, C. albicans isolates from the oral cavities of HIV-positive patients were less sensitive to histatin-5 than oral isolates from HIV-negative patients. Copyright © 2002 by The Editorial Council of The Journal of Prosthetic Dentistry.en_US
dc.languageengen_US
dc.publisherMosby, Inc. The Journal's web site is located at http://www.elsevier.com/locate/prosdenten_US
dc.relation.ispartofJournal of Prosthetic Dentistryen_US
dc.subject.meshAIDS-Related Opportunistic Infections - immunology - microbiology-
dc.subject.meshAntifungal Agents - administration and dosage - pharmacology-
dc.subject.meshCandida albicans - drug effects-
dc.subject.meshCandidiasis, Oral - immunology - microbiology-
dc.subject.meshSalivary Proteins and Peptides - administration and dosage - pharmacology-
dc.titleSusceptibility of Candida albicans isolates from the oral cavities of HIV-positive patients to histatin-5en_US
dc.typeArticleen_US
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1067/mpr.2002.127907en_US
dc.identifier.pmid12426495-
dc.identifier.scopuseid_2-s2.0-0036730267en_US
dc.identifier.hkuros75662-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036730267&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume88en_US
dc.identifier.issue3en_US
dc.identifier.spage263en_US
dc.identifier.epage267en_US
dc.identifier.isiWOS:000179317000005-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridNikawa, H=7006724162en_US
dc.identifier.scopusauthoridJin, C=26643041300en_US
dc.identifier.scopusauthoridMakihira, S=6603833899en_US
dc.identifier.scopusauthoridHamada, T=7401759268en_US
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_US

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