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Article: Granulocyte elastase, matrix metalloproteinase-8 and prostaglandin E 2 in gingival crevicular fluid in matched clinical sites in smokers and non-smokers with persistent periodontitis

TitleGranulocyte elastase, matrix metalloproteinase-8 and prostaglandin E 2 in gingival crevicular fluid in matched clinical sites in smokers and non-smokers with persistent periodontitis
Authors
Issue Date2002
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE
Citation
Journal Of Clinical Periodontology, 2002, v. 29 n. 5, p. 384-391 How to Cite?
AbstractBackground/aims: Smokers with persistent periodontitis may have granulocytes with impaired function. This study aimed to determine the levels of granulocyte elastase, matrix metalloproteinase-8 (MMP-8) and prostaglandin E2 (PGE2) in gingival crevicular fluid (GCF) in smokers and non-smokers with persistent periodontitis. Methods: We analyzed GCF from 70 matched sites in 29 periodontitis and 6 gingivitis sites in 34 subjects, 17 smokers, and 17 non-smokers. We also analyzed separately GCF from 28 of these subjects, 14 smokers and 14 non-smokers in 14 matched periodontitis sites. The following measurements were made: elastase complexed to α1- antitrypsin (EA-α1AT) and MMP-8 with ELISA, functional elastase with a chromogenic substrate, and PGE2 with radioimmunoassay ( 125I RIA). The significance of the findings was determined with Mann-Whitney test. Results: In the 29 matched periodontitis sites, smokers had significantly more functional elastase (p<0.005) and more EA-α1AT (p<0.05) than non-smokers. In the 14 matched periodontitis sites in 14 smokers and 14 non-smokers, the former had significantly more functional elastase than the latter (p<0.001). A significant correlation was found between EA-α1AT and MMP-8 in smokers (p<0.05) and non-smokers (p<0.001) and a positive correlation between levels of functional elastase and MMP-8 in non-smokers (r 2=0.98; p<0.001). Conclusions: Granulocyte function seems to be impaired in smokers with persistent periodontitis. The cells react to the bacterial challenge by releasing serine proteases, which reflect the degradation of connective tissue. The risk of progression of the disease is therefore higher in smokers with persistent periodontitis than in non-smokers. Copyright © Blackwell Munksgaard 2002.
Persistent Identifierhttp://hdl.handle.net/10722/154196
ISSN
2015 Impact Factor: 3.915
2015 SCImago Journal Rankings: 1.848
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSöder, Ben_US
dc.contributor.authorJin, LJen_US
dc.contributor.authorWickholm, Sen_US
dc.date.accessioned2012-08-08T08:23:49Z-
dc.date.available2012-08-08T08:23:49Z-
dc.date.issued2002en_US
dc.identifier.citationJournal Of Clinical Periodontology, 2002, v. 29 n. 5, p. 384-391en_US
dc.identifier.issn0303-6979en_US
dc.identifier.urihttp://hdl.handle.net/10722/154196-
dc.description.abstractBackground/aims: Smokers with persistent periodontitis may have granulocytes with impaired function. This study aimed to determine the levels of granulocyte elastase, matrix metalloproteinase-8 (MMP-8) and prostaglandin E2 (PGE2) in gingival crevicular fluid (GCF) in smokers and non-smokers with persistent periodontitis. Methods: We analyzed GCF from 70 matched sites in 29 periodontitis and 6 gingivitis sites in 34 subjects, 17 smokers, and 17 non-smokers. We also analyzed separately GCF from 28 of these subjects, 14 smokers and 14 non-smokers in 14 matched periodontitis sites. The following measurements were made: elastase complexed to α1- antitrypsin (EA-α1AT) and MMP-8 with ELISA, functional elastase with a chromogenic substrate, and PGE2 with radioimmunoassay ( 125I RIA). The significance of the findings was determined with Mann-Whitney test. Results: In the 29 matched periodontitis sites, smokers had significantly more functional elastase (p<0.005) and more EA-α1AT (p<0.05) than non-smokers. In the 14 matched periodontitis sites in 14 smokers and 14 non-smokers, the former had significantly more functional elastase than the latter (p<0.001). A significant correlation was found between EA-α1AT and MMP-8 in smokers (p<0.05) and non-smokers (p<0.001) and a positive correlation between levels of functional elastase and MMP-8 in non-smokers (r 2=0.98; p<0.001). Conclusions: Granulocyte function seems to be impaired in smokers with persistent periodontitis. The cells react to the bacterial challenge by releasing serine proteases, which reflect the degradation of connective tissue. The risk of progression of the disease is therefore higher in smokers with persistent periodontitis than in non-smokers. Copyright © Blackwell Munksgaard 2002.en_US
dc.languageengen_US
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPEen_US
dc.relation.ispartofJournal of Clinical Periodontologyen_US
dc.subject.meshCase-Control Studiesen_US
dc.subject.meshChromogenic Compounds - Diagnostic Useen_US
dc.subject.meshDental Plaque Indexen_US
dc.subject.meshDinoprostone - Analysisen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshFemaleen_US
dc.subject.meshGingival Crevicular Fluid - Chemistry - Enzymologyen_US
dc.subject.meshGingivitis - Enzymology - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshIodine Radioisotopes - Diagnostic Useen_US
dc.subject.meshLeukocyte Elastase - Analysisen_US
dc.subject.meshMaleen_US
dc.subject.meshMatrix Metalloproteinase 8 - Analysisen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPeriodontal Indexen_US
dc.subject.meshPeriodontitis - Enzymology - Metabolismen_US
dc.subject.meshRadioimmunoassayen_US
dc.subject.meshRadiopharmaceuticals - Diagnostic Useen_US
dc.subject.meshSerine Proteinase Inhibitors - Diagnostic Useen_US
dc.subject.meshSex Factorsen_US
dc.subject.meshSmoking - Metabolismen_US
dc.subject.meshStatistics, Nonparametricen_US
dc.subject.meshAlpha 1-Antitrypsin - Diagnostic Useen_US
dc.titleGranulocyte elastase, matrix metalloproteinase-8 and prostaglandin E 2 in gingival crevicular fluid in matched clinical sites in smokers and non-smokers with persistent periodontitisen_US
dc.typeArticleen_US
dc.identifier.emailJin, LJ:ljjin@hkucc.hku.hken_US
dc.identifier.authorityJin, LJ=rp00028en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1034/j.1600-051X.2002.290502.xen_US
dc.identifier.pmid12060420-
dc.identifier.scopuseid_2-s2.0-0036562186en_US
dc.identifier.hkuros67431-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036562186&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume29en_US
dc.identifier.issue5en_US
dc.identifier.spage384en_US
dc.identifier.epage391en_US
dc.identifier.isiWOS:000176702100002-
dc.publisher.placeDenmarken_US
dc.identifier.scopusauthoridSöder, B=6701795462en_US
dc.identifier.scopusauthoridJin, LJ=7403328850en_US
dc.identifier.scopusauthoridWickholm, S=6506767918en_US

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