File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Molecular heterogeneity of fluconazole-resistant and -susceptible oral candida albicans isolates within a single geographic locale

TitleMolecular heterogeneity of fluconazole-resistant and -susceptible oral candida albicans isolates within a single geographic locale
Authors
Issue Date2002
PublisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMIS
Citation
Apmis, 2002, v. 110 n. 4, p. 315-324 How to Cite?
AbstractThe emergence of drug-resistant Candida albicans in immunocompromised patients is common. A disconcerting aspect of this phenomenon is the rapid emergence of C. albicans strains that are resistant to a widely used azole drug, fluconazole (FLZ). To understand the origin of FLZ-resistant yeast isolates, we investigated molecular profiles of 20 geographically related oral C. albicans isolates using three genotyping methods: randomly amplified polymorphic DNA-PCR, with six different primers (OBU1, OBU2, OBU3 RSD6, RSD11 and RSD12); electrophoretic karyotyping by pulsed-field gel electrophoresis; and HinfI restriction fragment analysis. Of the 20 isolates studied, 10 were FLZ-resistant and originated from patients with oral candidosis with a history of FLZ therapy, and the remainder were FLZ susceptible from individuals with oral candidosis, but without a history of FLZ therapy. A composite genotype was generated for each strain by combining molecular types derived from the three independent molecular methods. The composite profiles indicated genetic diversity amongst both the FLZ-resistant as well as -sensitive isolates, and no specific features emerged distinguishing the drug-resistant and -sensitive groups. These observations cast doubt on the theory of a clonal origin of FLZ-resistant C. albicans isolates.
Persistent Identifierhttp://hdl.handle.net/10722/154186
ISSN
2015 Impact Factor: 1.933
2015 SCImago Journal Rankings: 0.855
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDassanayake, RSen_US
dc.contributor.authorEllepola, ANBen_US
dc.contributor.authorSamaranayake, YHen_US
dc.contributor.authorSamaranayak, LPen_US
dc.date.accessioned2012-08-08T08:23:46Z-
dc.date.available2012-08-08T08:23:46Z-
dc.date.issued2002en_US
dc.identifier.citationApmis, 2002, v. 110 n. 4, p. 315-324en_US
dc.identifier.issn0903-4641en_US
dc.identifier.urihttp://hdl.handle.net/10722/154186-
dc.description.abstractThe emergence of drug-resistant Candida albicans in immunocompromised patients is common. A disconcerting aspect of this phenomenon is the rapid emergence of C. albicans strains that are resistant to a widely used azole drug, fluconazole (FLZ). To understand the origin of FLZ-resistant yeast isolates, we investigated molecular profiles of 20 geographically related oral C. albicans isolates using three genotyping methods: randomly amplified polymorphic DNA-PCR, with six different primers (OBU1, OBU2, OBU3 RSD6, RSD11 and RSD12); electrophoretic karyotyping by pulsed-field gel electrophoresis; and HinfI restriction fragment analysis. Of the 20 isolates studied, 10 were FLZ-resistant and originated from patients with oral candidosis with a history of FLZ therapy, and the remainder were FLZ susceptible from individuals with oral candidosis, but without a history of FLZ therapy. A composite genotype was generated for each strain by combining molecular types derived from the three independent molecular methods. The composite profiles indicated genetic diversity amongst both the FLZ-resistant as well as -sensitive isolates, and no specific features emerged distinguishing the drug-resistant and -sensitive groups. These observations cast doubt on the theory of a clonal origin of FLZ-resistant C. albicans isolates.en_US
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMISen_US
dc.relation.ispartofAPMISen_US
dc.subject.meshAntifungal Agents - Pharmacologyen_US
dc.subject.meshCandida Albicans - Drug Effects - Genetics - Isolation & Purificationen_US
dc.subject.meshDrug Resistance, Fungal - Geneticsen_US
dc.subject.meshFluconazole - Pharmacologyen_US
dc.subject.meshGenetic Variationen_US
dc.subject.meshGenotypeen_US
dc.subject.meshHumansen_US
dc.subject.meshMouth Mucosa - Microbiologyen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.titleMolecular heterogeneity of fluconazole-resistant and -susceptible oral candida albicans isolates within a single geographic localeen_US
dc.typeArticleen_US
dc.identifier.emailSamaranayake, YH:hema@hkucc.hku.hken_US
dc.identifier.authoritySamaranayake, YH=rp00025en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1034/j.1600-0463.2002.100406.xen_US
dc.identifier.pmid12076267-
dc.identifier.scopuseid_2-s2.0-0036255511en_US
dc.identifier.hkuros65792-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036255511&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume110en_US
dc.identifier.issue4en_US
dc.identifier.spage315en_US
dc.identifier.epage324en_US
dc.identifier.isiWOS:000175508900006-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridDassanayake, RS=6603321318en_US
dc.identifier.scopusauthoridEllepola, ANB=6604060863en_US
dc.identifier.scopusauthoridSamaranayake, YH=6602677237en_US
dc.identifier.scopusauthoridSamaranayak, LP=18937186700en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats