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Article: Phylogenetic analysis of Prevotella nigrescens, Prevotella intermedia and Porphyromonas gingivalis clinical strains reveals a clear species clustering

TitlePhylogenetic analysis of Prevotella nigrescens, Prevotella intermedia and Porphyromonas gingivalis clinical strains reveals a clear species clustering
Authors
Issue Date2002
PublisherSociety for General Microbiology. The Journal's web site is located at http://ijs.sgmjournals.org
Citation
International Journal Of Systematic And Evolutionary Microbiology, 2002, v. 52 n. 4, p. 1391-1395 How to Cite?
AbstractPrevotella nigrescens, Prevotella intermedia and Porphyromonas gingivalis are oral pathogens from the family Bacteroidaceae, regularly isolated from cases of gingivitis and periodontitis. In this study, the phylogenetic variability of these three bacterial species was investigated by means of 16S rRNA (rrs) gene sequence comparisons of a set of epidemiologically and geographically diverse isolates. For each of the three species, the rrs gene sequences of 11 clinical isolates as well as the corresponding type strains was determined. Comparison of all rrs sequences obtained with those of closely related species revealed a clear clustering of species, with only a little intraspecies variability but a clear difference in the rrs gene with respect to the next related taxon. The results indicate that the three species form stable, homogeneous genetic groups, which favours an rrs-based species identification of these oral pathogens. This is especially useful given the 7% sequence divergence between Prevotella intermedia and Prevotella nigrescens, since phenotypic distinction between the two Prevotella species is inconsistent or involves techniques not applicable in routine identification.
Persistent Identifierhttp://hdl.handle.net/10722/154178
ISSN
2015 Impact Factor: 2.439
2015 SCImago Journal Rankings: 1.088
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKuhnert, Pen_US
dc.contributor.authorFrey, Jen_US
dc.contributor.authorLang, NPen_US
dc.contributor.authorMayfield, Len_US
dc.date.accessioned2012-08-08T08:23:43Z-
dc.date.available2012-08-08T08:23:43Z-
dc.date.issued2002en_US
dc.identifier.citationInternational Journal Of Systematic And Evolutionary Microbiology, 2002, v. 52 n. 4, p. 1391-1395en_US
dc.identifier.issn1466-5026en_US
dc.identifier.urihttp://hdl.handle.net/10722/154178-
dc.description.abstractPrevotella nigrescens, Prevotella intermedia and Porphyromonas gingivalis are oral pathogens from the family Bacteroidaceae, regularly isolated from cases of gingivitis and periodontitis. In this study, the phylogenetic variability of these three bacterial species was investigated by means of 16S rRNA (rrs) gene sequence comparisons of a set of epidemiologically and geographically diverse isolates. For each of the three species, the rrs gene sequences of 11 clinical isolates as well as the corresponding type strains was determined. Comparison of all rrs sequences obtained with those of closely related species revealed a clear clustering of species, with only a little intraspecies variability but a clear difference in the rrs gene with respect to the next related taxon. The results indicate that the three species form stable, homogeneous genetic groups, which favours an rrs-based species identification of these oral pathogens. This is especially useful given the 7% sequence divergence between Prevotella intermedia and Prevotella nigrescens, since phenotypic distinction between the two Prevotella species is inconsistent or involves techniques not applicable in routine identification.en_US
dc.languageengen_US
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://ijs.sgmjournals.orgen_US
dc.relation.ispartofInternational Journal of Systematic and Evolutionary Microbiologyen_US
dc.subject.meshBacteroidaceae Infections - Microbiologyen_US
dc.subject.meshCluster Analysisen_US
dc.subject.meshDna, Ribosomal - Analysisen_US
dc.subject.meshGingivitis - Microbiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPeriodontitis - Microbiologyen_US
dc.subject.meshPhylogenyen_US
dc.subject.meshPorphyromonas Gingivalis - Classification - Geneticsen_US
dc.subject.meshPrevotella - Classification - Geneticsen_US
dc.subject.meshPrevotella Intermedia - Classification - Geneticsen_US
dc.subject.meshRna, Ribosomal, 16S - Geneticsen_US
dc.subject.meshSequence Analysis, Dnaen_US
dc.titlePhylogenetic analysis of Prevotella nigrescens, Prevotella intermedia and Porphyromonas gingivalis clinical strains reveals a clear species clusteringen_US
dc.typeArticleen_US
dc.identifier.emailLang, NP:nplang@hkucc.hku.hken_US
dc.identifier.authorityLang, NP=rp00031en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1099/ijs.0.02021-0en_US
dc.identifier.pmid12148655-
dc.identifier.scopuseid_2-s2.0-0036022494en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036022494&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume52en_US
dc.identifier.issue4en_US
dc.identifier.spage1391en_US
dc.identifier.epage1395en_US
dc.identifier.isiWOS:000176925200045-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridKuhnert, P=7004464689en_US
dc.identifier.scopusauthoridFrey, J=7202811675en_US
dc.identifier.scopusauthoridLang, NP=7201577367en_US
dc.identifier.scopusauthoridMayfield, L=7004160753en_US

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