File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Evaluation of polyene-azole antagonism in liquid cultures of Candida albicans using an automated turbidometric method

TitleEvaluation of polyene-azole antagonism in liquid cultures of Candida albicans using an automated turbidometric method
Authors
Issue Date2001
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/CHE
Citation
Chemotherapy, 2001, v. 47 n. 4, p. 279-291 How to Cite?
AbstractBackground: A computerized machine, SPECTRAmax 340, was used to evaluate the recently reported phenomenon of antagonism of the polyene amphotericin B (AMB) in Candida albicans pre-exposed to the triazole fluconazole (FLZ). Methods: We investigated growth inhibition by varying concentrations of AMB in seven isolates of C. albicans pre-exposed to FLZ (50 μg/ml) for 18 h. All isolates were obtained on sequential visits from human immunodeficiency virus-infected patients not treated with FLZ. Results: Antagonism of AMB activity was observed in 5, 4, 2 and a single isolate for 0.5, 1, 2 and 3 μg/ml of the antifungal, respectively. In the majority of Candida isolates, antagonism was seen within a concentration range of 0.5-1.0 μg/ml AMB; 1 Candida strain (HK1-Sa) was resistant to 3 μg/ml AMB. Higher concentrations of AMB (>3 μg/ml) killed both the controls and FLZ-pre-exposed Candida cells. No significant differences were observed between the periods of antagonism observed for any of the sequential isolates or for any of the AMB concentrations or in the maxima of the growth curves obtained for all Candida isolates. Conclusion: We conclude that the SPECTRAmax system is a useful tool to evaluate in vitro pharmacodynamic interactions between antifungal regimens within a fluid culture system, and provides information that cannot be obtained using traditional plate assay systems. Copyright © 2001 S. Karger AG, Basel.
Persistent Identifierhttp://hdl.handle.net/10722/154137
ISSN
2015 Impact Factor: 0.992
2015 SCImago Journal Rankings: 0.630
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSamaranayake, YHen_US
dc.contributor.authorSamaranayake, LPen_US
dc.contributor.authorYeung, KWSen_US
dc.date.accessioned2012-08-08T08:23:26Z-
dc.date.available2012-08-08T08:23:26Z-
dc.date.issued2001en_US
dc.identifier.citationChemotherapy, 2001, v. 47 n. 4, p. 279-291en_US
dc.identifier.issn0009-3157en_US
dc.identifier.urihttp://hdl.handle.net/10722/154137-
dc.description.abstractBackground: A computerized machine, SPECTRAmax 340, was used to evaluate the recently reported phenomenon of antagonism of the polyene amphotericin B (AMB) in Candida albicans pre-exposed to the triazole fluconazole (FLZ). Methods: We investigated growth inhibition by varying concentrations of AMB in seven isolates of C. albicans pre-exposed to FLZ (50 μg/ml) for 18 h. All isolates were obtained on sequential visits from human immunodeficiency virus-infected patients not treated with FLZ. Results: Antagonism of AMB activity was observed in 5, 4, 2 and a single isolate for 0.5, 1, 2 and 3 μg/ml of the antifungal, respectively. In the majority of Candida isolates, antagonism was seen within a concentration range of 0.5-1.0 μg/ml AMB; 1 Candida strain (HK1-Sa) was resistant to 3 μg/ml AMB. Higher concentrations of AMB (>3 μg/ml) killed both the controls and FLZ-pre-exposed Candida cells. No significant differences were observed between the periods of antagonism observed for any of the sequential isolates or for any of the AMB concentrations or in the maxima of the growth curves obtained for all Candida isolates. Conclusion: We conclude that the SPECTRAmax system is a useful tool to evaluate in vitro pharmacodynamic interactions between antifungal regimens within a fluid culture system, and provides information that cannot be obtained using traditional plate assay systems. Copyright © 2001 S. Karger AG, Basel.en_US
dc.languageengen_US
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/CHEen_US
dc.relation.ispartofChemotherapyen_US
dc.subject.meshAmphotericin B - Antagonists & Inhibitors - Pharmacologyen_US
dc.subject.meshAnalysis Of Varianceen_US
dc.subject.meshAntifungal Agents - Antagonists & Inhibitors - Pharmacologyen_US
dc.subject.meshCandida Albicans - Drug Effects - Growth & Developmenten_US
dc.subject.meshDrug Evaluation, Preclinicalen_US
dc.subject.meshDrug Interactionsen_US
dc.subject.meshDrug Therapy, Combinationen_US
dc.subject.meshFluconazole - Pharmacologyen_US
dc.subject.meshMicrobial Sensitivity Testsen_US
dc.subject.meshSpecies Specificityen_US
dc.titleEvaluation of polyene-azole antagonism in liquid cultures of Candida albicans using an automated turbidometric methoden_US
dc.typeArticleen_US
dc.identifier.emailSamaranayake, YH:hema@hkucc.hku.hken_US
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_US
dc.identifier.authoritySamaranayake, YH=rp00025en_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1159/000048535en_US
dc.identifier.pmid11399865-
dc.identifier.scopuseid_2-s2.0-0034962180en_US
dc.identifier.hkuros57358-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034962180&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume47en_US
dc.identifier.issue4en_US
dc.identifier.spage279en_US
dc.identifier.epage291en_US
dc.identifier.isiWOS:000169068800008-
dc.publisher.placeSwitzerlanden_US
dc.identifier.scopusauthoridSamaranayake, YH=6602677237en_US
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_US
dc.identifier.scopusauthoridYeung, KWS=13304249300en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats