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Article: Genomic diversity of oral Candida krusei isolates as revealed by DNA fingerprinting and electrophoretic karyotyping

TitleGenomic diversity of oral Candida krusei isolates as revealed by DNA fingerprinting and electrophoretic karyotyping
Authors
Issue Date2000
PublisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMIS
Citation
APMIS, 2000, v. 108 n. 10, p. 697-704 How to Cite?
AbstractCandida krusei is receiving increasing attention as an important human pathogen, especially in compromised patients, who frequently manifest with multiepisodes of candidosis. As there is scant information on the genetic diversity of this pathogen the present study was undertaken to establish its genetic profiles using three different typing methods: PFGE (pulsed-field gel electrophoresis), RFLP (restriction fragment length polymorphism), RAPD (randomly amplified polymorphic DNA). When 11 oral isolates of C. krusei were molecular typed by PFGE, 3 to 5 chromosomes with sizes ranging from 1000 kb to 3000 kb per isolate were revealed. All isolates produced a single bright band at approximately 1,100 kb and two to three bands between 2,500 kb and 3,000 kb, demonstrating 5 different karyotypes. RFLP with HinfI yielded 9 different genotypes, while DNA fingerprinting by RAPD with 3 primers (RSD6 (5′GCGATCCCCA3′), RSD7 (5′AGTGAATTCG CGGTGAGATGCC3′) and RSD12 (5′GCATATCAATAAGC GCAGGAAAAG 3′)), resulted in 8, 3 and 11 different genotypes, respectively. This study provides evidence hitherto unavailable on the genetic polymorphism of C. krusei isolates colonizing the oral niche under different clinical conditions. Such genotypic polymorphism should help strain delineation in epidemiologic surveillance of either nosocomial or community outbreaks of C. krusei infections.
Persistent Identifierhttp://hdl.handle.net/10722/154124
ISSN
2015 Impact Factor: 1.933
2015 SCImago Journal Rankings: 0.855
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDassanayake, RSen_US
dc.contributor.authorSamaranayake, YHen_US
dc.contributor.authorSamaranayake, LPen_US
dc.date.accessioned2012-08-08T08:23:23Z-
dc.date.available2012-08-08T08:23:23Z-
dc.date.issued2000en_US
dc.identifier.citationAPMIS, 2000, v. 108 n. 10, p. 697-704en_US
dc.identifier.issn0903-4641en_US
dc.identifier.urihttp://hdl.handle.net/10722/154124-
dc.description.abstractCandida krusei is receiving increasing attention as an important human pathogen, especially in compromised patients, who frequently manifest with multiepisodes of candidosis. As there is scant information on the genetic diversity of this pathogen the present study was undertaken to establish its genetic profiles using three different typing methods: PFGE (pulsed-field gel electrophoresis), RFLP (restriction fragment length polymorphism), RAPD (randomly amplified polymorphic DNA). When 11 oral isolates of C. krusei were molecular typed by PFGE, 3 to 5 chromosomes with sizes ranging from 1000 kb to 3000 kb per isolate were revealed. All isolates produced a single bright band at approximately 1,100 kb and two to three bands between 2,500 kb and 3,000 kb, demonstrating 5 different karyotypes. RFLP with HinfI yielded 9 different genotypes, while DNA fingerprinting by RAPD with 3 primers (RSD6 (5′GCGATCCCCA3′), RSD7 (5′AGTGAATTCG CGGTGAGATGCC3′) and RSD12 (5′GCATATCAATAAGC GCAGGAAAAG 3′)), resulted in 8, 3 and 11 different genotypes, respectively. This study provides evidence hitherto unavailable on the genetic polymorphism of C. krusei isolates colonizing the oral niche under different clinical conditions. Such genotypic polymorphism should help strain delineation in epidemiologic surveillance of either nosocomial or community outbreaks of C. krusei infections.en_US
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMISen_US
dc.relation.ispartofAPMISen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCandida - Genetics - Isolation & Purification - Pathogenicityen_US
dc.subject.meshCandidiasis, Oral - Microbiologyen_US
dc.subject.meshDna Fingerprintingen_US
dc.subject.meshDna Primers - Geneticsen_US
dc.subject.meshDna, Fungal - Genetics - Isolation & Purificationen_US
dc.subject.meshElectrophoresis, Gel, Pulsed-Fielden_US
dc.subject.meshGenetic Variationen_US
dc.subject.meshGenome, Fungalen_US
dc.subject.meshHumansen_US
dc.subject.meshKaryotypingen_US
dc.subject.meshOpportunistic Infections - Microbiologyen_US
dc.subject.meshPolymorphism, Geneticen_US
dc.subject.meshPolymorphism, Restriction Fragment Lengthen_US
dc.subject.meshRandom Amplified Polymorphic Dna Techniqueen_US
dc.subject.meshVirulenceen_US
dc.titleGenomic diversity of oral Candida krusei isolates as revealed by DNA fingerprinting and electrophoretic karyotypingen_US
dc.typeArticleen_US
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0903-4641&volume=108&spage=697&epage=704&date=2001&atitle=Genomic+diversity+of+oral+Candida+krusei+isolates+as+revealed+by+DNA+finger+printing+and+electrophoretic+karyotyping-
dc.identifier.emailSamaranayake, YH: hema@hkucc.hku.hken_US
dc.identifier.emailSamaranayake, LP: lakshman@hku.hken_US
dc.identifier.authoritySamaranayake, YH=rp00025en_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1034/j.1600-0463.2000.d01-17.x-
dc.identifier.pmid11200825-
dc.identifier.scopuseid_2-s2.0-0034517815en_US
dc.identifier.hkuros56550-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034517815&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume108en_US
dc.identifier.issue10en_US
dc.identifier.spage697en_US
dc.identifier.epage704en_US
dc.identifier.isiWOS:000166191400009-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridDassanayake, RS=6603321318en_US
dc.identifier.scopusauthoridSamaranayake, YH=6602677237en_US
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_US

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