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Article: Interleukin-8 and Granulocyte Elastase in Gingival Crevicular Fluid in Relation to Periodontopathogens in Untreated Adult Periodontitis

TitleInterleukin-8 and Granulocyte Elastase in Gingival Crevicular Fluid in Relation to Periodontopathogens in Untreated Adult Periodontitis
Authors
Issue Date2000
PublisherAmerican Academy of Periodontology. The Journal's web site is located at http://www.perio.org
Citation
Journal Of Periodontology, 2000, v. 71 n. 6, p. 929-939 How to Cite?
AbstractBackground: This study aimed to determine the relationships among interleukin (IL)-8 and granulocyte elastase levels in gingival crevicular fluid (GCF) and the concomitant presence of periodontopathogens in untreated adult periodontitis. Methods: GCF and subgingival plaque samples were collected from 16 patients with untreated adult periodontitis and 10 healthy control subjects. IL-8 levels were determined by enzyme-linked immunosorbent assay (ELISA). Granulocyte elastase was analyzed with a neutrophilic granulocyte-specific, low molecular weight and chromogenic substrate, L-pyroglutamyl-L-prolyl-L-valine-p-nitroanilide, and the maximal rate of elastase activity (MR-EA) was calculated. Five DNA probes were used to detect the presence of A. actinomycetemcomitans (A.a.), B. forsythus (B.f.), P. gingivalis (P.g.), P. intermedia (P.i.), and T. denticola (T.d.). Results: Lower IL-8 concentrations and higher granulocyte elastase activities were found in patients than in healthy controls as well as in diseased conditions co-infected with B.f., P.g., P.i., and T.d. as compared to healthy conditions without the target species (P <0.05). IL-8 concentrations were positively correlated with MR-EA levels in the periodontitis conditions co-infected with B.f., P.g., P.i., and T.d. (P <0.05). A wide range of IL-8 concentrations was found among 15 patients when the periodontitis condition was characterized by co-infection with B.f., P.g., P.i., and T.d. MR-EA levels in the high IL-8 group of subjects were significantly higher than those in the low IL-8 group of subjects (P <0.01). Conclusions: The present study shows that the local host-bacteria interactions in untreated periodontitis are diverse in terms of the intensity of inflammatory responses measured by IL-8-related granulocyte elastase activity in GCF. This might reflect different phases of the inflammatory response due to shifts in host-bacteria interactions and therefore be indicative of a range of periodontal disease activity levels.
Persistent Identifierhttp://hdl.handle.net/10722/154107
ISSN
2015 Impact Factor: 2.844
2015 SCImago Journal Rankings: 1.070
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJin, Len_US
dc.contributor.authorSöder, Ben_US
dc.contributor.authorCorbet, EFen_US
dc.date.accessioned2012-08-08T08:23:17Z-
dc.date.available2012-08-08T08:23:17Z-
dc.date.issued2000en_US
dc.identifier.citationJournal Of Periodontology, 2000, v. 71 n. 6, p. 929-939en_US
dc.identifier.issn0022-3492en_US
dc.identifier.urihttp://hdl.handle.net/10722/154107-
dc.description.abstractBackground: This study aimed to determine the relationships among interleukin (IL)-8 and granulocyte elastase levels in gingival crevicular fluid (GCF) and the concomitant presence of periodontopathogens in untreated adult periodontitis. Methods: GCF and subgingival plaque samples were collected from 16 patients with untreated adult periodontitis and 10 healthy control subjects. IL-8 levels were determined by enzyme-linked immunosorbent assay (ELISA). Granulocyte elastase was analyzed with a neutrophilic granulocyte-specific, low molecular weight and chromogenic substrate, L-pyroglutamyl-L-prolyl-L-valine-p-nitroanilide, and the maximal rate of elastase activity (MR-EA) was calculated. Five DNA probes were used to detect the presence of A. actinomycetemcomitans (A.a.), B. forsythus (B.f.), P. gingivalis (P.g.), P. intermedia (P.i.), and T. denticola (T.d.). Results: Lower IL-8 concentrations and higher granulocyte elastase activities were found in patients than in healthy controls as well as in diseased conditions co-infected with B.f., P.g., P.i., and T.d. as compared to healthy conditions without the target species (P <0.05). IL-8 concentrations were positively correlated with MR-EA levels in the periodontitis conditions co-infected with B.f., P.g., P.i., and T.d. (P <0.05). A wide range of IL-8 concentrations was found among 15 patients when the periodontitis condition was characterized by co-infection with B.f., P.g., P.i., and T.d. MR-EA levels in the high IL-8 group of subjects were significantly higher than those in the low IL-8 group of subjects (P <0.01). Conclusions: The present study shows that the local host-bacteria interactions in untreated periodontitis are diverse in terms of the intensity of inflammatory responses measured by IL-8-related granulocyte elastase activity in GCF. This might reflect different phases of the inflammatory response due to shifts in host-bacteria interactions and therefore be indicative of a range of periodontal disease activity levels.en_US
dc.languageengen_US
dc.publisherAmerican Academy of Periodontology. The Journal's web site is located at http://www.perio.orgen_US
dc.relation.ispartofJournal of Periodontologyen_US
dc.subject.meshActinobacillus Infections - Enzymology - Immunologyen_US
dc.subject.meshActinobacillus Actinomycetemcomitans - Enzymology - Immunologyen_US
dc.subject.meshAdulten_US
dc.subject.meshAnalysis Of Varianceen_US
dc.subject.meshBacteria - Enzymology - Immunologyen_US
dc.subject.meshBacteroidaceae Infections - Enzymology - Immunologyen_US
dc.subject.meshBacteroides - Classificationen_US
dc.subject.meshBacteroides Infections - Enzymology - Immunologyen_US
dc.subject.meshDental Plaque - Microbiologyen_US
dc.subject.meshGingival Crevicular Fluid - Enzymology - Immunology - Microbiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshInterleukin-8 - Analysisen_US
dc.subject.meshLeukocyte Elastase - Analysisen_US
dc.subject.meshLinear Modelsen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPeriodontitis - Enzymology - Immunology - Microbiologyen_US
dc.subject.meshPorphyromonas Gingivalis - Enzymology - Immunologyen_US
dc.subject.meshPrevotella Intermedia - Enzymology - Immunologyen_US
dc.subject.meshStatistics, Nonparametricen_US
dc.subject.meshTreponema - Enzymology - Immunologyen_US
dc.subject.meshTreponemal Infections - Enzymology - Immunologyen_US
dc.titleInterleukin-8 and Granulocyte Elastase in Gingival Crevicular Fluid in Relation to Periodontopathogens in Untreated Adult Periodontitisen_US
dc.typeArticleen_US
dc.identifier.emailJin, L:ljjin@hkucc.hku.hken_US
dc.identifier.emailCorbet, EF:efcorbet@hku.hken_US
dc.identifier.authorityJin, L=rp00028en_US
dc.identifier.authorityCorbet, EF=rp00005en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid10914796-
dc.identifier.scopuseid_2-s2.0-0034198829en_US
dc.identifier.hkuros49454-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034198829&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume71en_US
dc.identifier.issue6en_US
dc.identifier.spage929en_US
dc.identifier.epage939en_US
dc.identifier.isiWOS:000167489500006-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridJin, L=7403328850en_US
dc.identifier.scopusauthoridSöder, B=6701795462en_US
dc.identifier.scopusauthoridCorbet, EF=35609873200en_US

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