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- Publisher Website: 10.1902/jop.2000.71.6.929
- Scopus: eid_2-s2.0-0034198829
- PMID: 10914796
- WOS: WOS:000167489500006
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Article: Interleukin-8 and Granulocyte Elastase in Gingival Crevicular Fluid in Relation to Periodontopathogens in Untreated Adult Periodontitis
Title | Interleukin-8 and Granulocyte Elastase in Gingival Crevicular Fluid in Relation to Periodontopathogens in Untreated Adult Periodontitis |
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Authors | |
Keywords | Elastase, granulocyte Gingival crevicular fluid/analysis Inflammatory response Interleukin-8 Periodontitis/pathogenesis |
Issue Date | 2000 |
Publisher | American Academy of Periodontology. The Journal's web site is located at http://www.perio.org |
Citation | Journal Of Periodontology, 2000, v. 71 n. 6, p. 929-939 How to Cite? |
Abstract | Background: This study aimed to determine the relationships among interleukin (IL)-8 and granulocyte elastase levels in gingival crevicular fluid (GCF) and the concomitant presence of periodontopathogens in untreated adult periodontitis. Methods: GCF and subgingival plaque samples were collected from 16 patients with untreated adult periodontitis and 10 healthy control subjects. IL-8 levels were determined by enzyme-linked immunosorbent assay (ELISA). Granulocyte elastase was analyzed with a neutrophilic granulocyte-specific, low molecular weight and chromogenic substrate, L-pyroglutamyl-L-prolyl-L-valine-p-nitroanilide, and the maximal rate of elastase activity (MR-EA) was calculated. Five DNA probes were used to detect the presence of A. actinomycetemcomitans (A.a.), B. forsythus (B.f.), P. gingivalis (P.g.), P. intermedia (P.i.), and T. denticola (T.d.). Results: Lower IL-8 concentrations and higher granulocyte elastase activities were found in patients than in healthy controls as well as in diseased conditions co-infected with B.f., P.g., P.i., and T.d. as compared to healthy conditions without the target species (P <0.05). IL-8 concentrations were positively correlated with MR-EA levels in the periodontitis conditions co-infected with B.f., P.g., P.i., and T.d. (P <0.05). A wide range of IL-8 concentrations was found among 15 patients when the periodontitis condition was characterized by co-infection with B.f., P.g., P.i., and T.d. MR-EA levels in the high IL-8 group of subjects were significantly higher than those in the low IL-8 group of subjects (P <0.01). Conclusions: The present study shows that the local host-bacteria interactions in untreated periodontitis are diverse in terms of the intensity of inflammatory responses measured by IL-8-related granulocyte elastase activity in GCF. This might reflect different phases of the inflammatory response due to shifts in host-bacteria interactions and therefore be indicative of a range of periodontal disease activity levels. |
Persistent Identifier | http://hdl.handle.net/10722/154107 |
ISSN | 2023 Impact Factor: 4.2 2023 SCImago Journal Rankings: 1.362 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Jin, L | en_US |
dc.contributor.author | Söder, B | en_US |
dc.contributor.author | Corbet, EF | en_US |
dc.date.accessioned | 2012-08-08T08:23:17Z | - |
dc.date.available | 2012-08-08T08:23:17Z | - |
dc.date.issued | 2000 | en_US |
dc.identifier.citation | Journal Of Periodontology, 2000, v. 71 n. 6, p. 929-939 | en_US |
dc.identifier.issn | 0022-3492 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/154107 | - |
dc.description.abstract | Background: This study aimed to determine the relationships among interleukin (IL)-8 and granulocyte elastase levels in gingival crevicular fluid (GCF) and the concomitant presence of periodontopathogens in untreated adult periodontitis. Methods: GCF and subgingival plaque samples were collected from 16 patients with untreated adult periodontitis and 10 healthy control subjects. IL-8 levels were determined by enzyme-linked immunosorbent assay (ELISA). Granulocyte elastase was analyzed with a neutrophilic granulocyte-specific, low molecular weight and chromogenic substrate, L-pyroglutamyl-L-prolyl-L-valine-p-nitroanilide, and the maximal rate of elastase activity (MR-EA) was calculated. Five DNA probes were used to detect the presence of A. actinomycetemcomitans (A.a.), B. forsythus (B.f.), P. gingivalis (P.g.), P. intermedia (P.i.), and T. denticola (T.d.). Results: Lower IL-8 concentrations and higher granulocyte elastase activities were found in patients than in healthy controls as well as in diseased conditions co-infected with B.f., P.g., P.i., and T.d. as compared to healthy conditions without the target species (P <0.05). IL-8 concentrations were positively correlated with MR-EA levels in the periodontitis conditions co-infected with B.f., P.g., P.i., and T.d. (P <0.05). A wide range of IL-8 concentrations was found among 15 patients when the periodontitis condition was characterized by co-infection with B.f., P.g., P.i., and T.d. MR-EA levels in the high IL-8 group of subjects were significantly higher than those in the low IL-8 group of subjects (P <0.01). Conclusions: The present study shows that the local host-bacteria interactions in untreated periodontitis are diverse in terms of the intensity of inflammatory responses measured by IL-8-related granulocyte elastase activity in GCF. This might reflect different phases of the inflammatory response due to shifts in host-bacteria interactions and therefore be indicative of a range of periodontal disease activity levels. | en_US |
dc.language | eng | en_US |
dc.publisher | American Academy of Periodontology. The Journal's web site is located at http://www.perio.org | en_US |
dc.relation.ispartof | Journal of Periodontology | en_US |
dc.subject | Elastase, granulocyte | - |
dc.subject | Gingival crevicular fluid/analysis | - |
dc.subject | Inflammatory response | - |
dc.subject | Interleukin-8 | - |
dc.subject | Periodontitis/pathogenesis | - |
dc.subject.mesh | Actinobacillus Infections - Enzymology - Immunology | en_US |
dc.subject.mesh | Actinobacillus Actinomycetemcomitans - Enzymology - Immunology | en_US |
dc.subject.mesh | Adult | en_US |
dc.subject.mesh | Analysis Of Variance | en_US |
dc.subject.mesh | Bacteria - Enzymology - Immunology | en_US |
dc.subject.mesh | Bacteroidaceae Infections - Enzymology - Immunology | en_US |
dc.subject.mesh | Bacteroides - Classification | en_US |
dc.subject.mesh | Bacteroides Infections - Enzymology - Immunology | en_US |
dc.subject.mesh | Dental Plaque - Microbiology | en_US |
dc.subject.mesh | Gingival Crevicular Fluid - Enzymology - Immunology - Microbiology | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Interleukin-8 - Analysis | en_US |
dc.subject.mesh | Leukocyte Elastase - Analysis | en_US |
dc.subject.mesh | Linear Models | en_US |
dc.subject.mesh | Middle Aged | en_US |
dc.subject.mesh | Periodontitis - Enzymology - Immunology - Microbiology | en_US |
dc.subject.mesh | Porphyromonas Gingivalis - Enzymology - Immunology | en_US |
dc.subject.mesh | Prevotella Intermedia - Enzymology - Immunology | en_US |
dc.subject.mesh | Statistics, Nonparametric | en_US |
dc.subject.mesh | Treponema - Enzymology - Immunology | en_US |
dc.subject.mesh | Treponemal Infections - Enzymology - Immunology | en_US |
dc.title | Interleukin-8 and Granulocyte Elastase in Gingival Crevicular Fluid in Relation to Periodontopathogens in Untreated Adult Periodontitis | en_US |
dc.type | Article | en_US |
dc.identifier.email | Jin, L:ljjin@hkucc.hku.hk | en_US |
dc.identifier.email | Corbet, EF:efcorbet@hku.hk | en_US |
dc.identifier.authority | Jin, L=rp00028 | en_US |
dc.identifier.authority | Corbet, EF=rp00005 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1902/jop.2000.71.6.929 | - |
dc.identifier.pmid | 10914796 | - |
dc.identifier.scopus | eid_2-s2.0-0034198829 | en_US |
dc.identifier.hkuros | 49454 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0034198829&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 71 | en_US |
dc.identifier.issue | 6 | en_US |
dc.identifier.spage | 929 | en_US |
dc.identifier.epage | 939 | en_US |
dc.identifier.isi | WOS:000167489500006 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Jin, L=7403328850 | en_US |
dc.identifier.scopusauthorid | Söder, B=6701795462 | en_US |
dc.identifier.scopusauthorid | Corbet, EF=35609873200 | en_US |
dc.identifier.issnl | 0022-3492 | - |