File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Sub-therapeutic exposure to polyene antimycotics elicits a post-antifungal effect (PAFE), and depresses the cell surface hydrophobicity of oral Candida albicans isolates

TitleSub-therapeutic exposure to polyene antimycotics elicits a post-antifungal effect (PAFE), and depresses the cell surface hydrophobicity of oral Candida albicans isolates
Authors
Issue Date2000
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JOPM
Citation
Journal Of Oral Pathology And Medicine, 2000, v. 29 n. 5, p. 206-213 How to Cite?
AbstractPost-antifungal effect (PAFE) is defined as the suppression of growth that persists following limited exposure of fungi to antimycotics and subsequent removal of the drug. The fungal pathogen Candida albicans is the major aetiologic agent of oral candidosis, and the cell surface hydrophobicity (CSH) of this yeast is considered a critical factor contributing to its colonisation potential. As the concentration of topically prescribed antifungals reach sub-therapeutic levels at dosage intervals, the study of the polyene-induced PAFE and its impact on the CSH of oral C. albicans should be of clinical relevance. Hence the aims of this investigation were to measure the PAFE and CSH of 12 isolates of C. albicans following limited exposure (1 h) to nystatin and amphotericin B and also to investigate the ultrastructural features of yeast cells following such antifungal exposure. The yeasts were exposed to sub-lethal concentrations of nystatin (x 2 MIC) and amphotericin B (x 2 MIC) for a period of 1 h. Following subsequent removal of the drug, the PAFE and the CSH of the isolates were assessed by a turbidometric measurement of growth and a biphasic aqueous-hydrocarbon assay, respectively. The mean duration of PAFE of nystatin and amphotericin B were 5.99 (±0.49) h and 8.73 (±0.93) h, respectively, while the reduction in CSH following exposure to these drugs were 17.32% (P < 0.05 for 83% of the isolates) and 14.26% (P < 0.05 for 66% of the isolates), respectively. On scanning electron microscopy the exposed cells were seen to undergo collapse of the internal cell membrane, leaving an intact cell wall, while a proportion of cells were deflated. Some cells showed intense puckering of the cell wall, resulting in a mulberry appearance. Taken together, these data elucidate additional mechanisms by which polyene antimycotics may operate in vivo to suppress candidal pathogenicity.
Persistent Identifierhttp://hdl.handle.net/10722/154091
ISSN
2015 Impact Factor: 1.859
2015 SCImago Journal Rankings: 0.731
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorEgusa, Hen_US
dc.contributor.authorEllepola, ANBen_US
dc.contributor.authorNikawa, Hen_US
dc.contributor.authorHamada, Ten_US
dc.contributor.authorSamaranayake, LPen_US
dc.date.accessioned2012-08-08T08:23:13Z-
dc.date.available2012-08-08T08:23:13Z-
dc.date.issued2000en_US
dc.identifier.citationJournal Of Oral Pathology And Medicine, 2000, v. 29 n. 5, p. 206-213en_US
dc.identifier.issn0904-2512en_US
dc.identifier.urihttp://hdl.handle.net/10722/154091-
dc.description.abstractPost-antifungal effect (PAFE) is defined as the suppression of growth that persists following limited exposure of fungi to antimycotics and subsequent removal of the drug. The fungal pathogen Candida albicans is the major aetiologic agent of oral candidosis, and the cell surface hydrophobicity (CSH) of this yeast is considered a critical factor contributing to its colonisation potential. As the concentration of topically prescribed antifungals reach sub-therapeutic levels at dosage intervals, the study of the polyene-induced PAFE and its impact on the CSH of oral C. albicans should be of clinical relevance. Hence the aims of this investigation were to measure the PAFE and CSH of 12 isolates of C. albicans following limited exposure (1 h) to nystatin and amphotericin B and also to investigate the ultrastructural features of yeast cells following such antifungal exposure. The yeasts were exposed to sub-lethal concentrations of nystatin (x 2 MIC) and amphotericin B (x 2 MIC) for a period of 1 h. Following subsequent removal of the drug, the PAFE and the CSH of the isolates were assessed by a turbidometric measurement of growth and a biphasic aqueous-hydrocarbon assay, respectively. The mean duration of PAFE of nystatin and amphotericin B were 5.99 (±0.49) h and 8.73 (±0.93) h, respectively, while the reduction in CSH following exposure to these drugs were 17.32% (P < 0.05 for 83% of the isolates) and 14.26% (P < 0.05 for 66% of the isolates), respectively. On scanning electron microscopy the exposed cells were seen to undergo collapse of the internal cell membrane, leaving an intact cell wall, while a proportion of cells were deflated. Some cells showed intense puckering of the cell wall, resulting in a mulberry appearance. Taken together, these data elucidate additional mechanisms by which polyene antimycotics may operate in vivo to suppress candidal pathogenicity.en_US
dc.languageengen_US
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JOPMen_US
dc.relation.ispartofJournal of Oral Pathology and Medicineen_US
dc.subject.meshAmphotericin B - Pharmacologyen_US
dc.subject.meshAntifungal Agents - Pharmacologyen_US
dc.subject.meshCandida Albicans - Drug Effects - Pathogenicityen_US
dc.subject.meshCandidiasis, Oral - Drug Therapy - Microbiologyen_US
dc.subject.meshCell Membrane - Drug Effectsen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshHumansen_US
dc.subject.meshMicrobial Sensitivity Testsen_US
dc.subject.meshMicroscopy, Electron, Scanningen_US
dc.subject.meshNystatin - Pharmacologyen_US
dc.subject.meshVirulence - Drug Effectsen_US
dc.titleSub-therapeutic exposure to polyene antimycotics elicits a post-antifungal effect (PAFE), and depresses the cell surface hydrophobicity of oral Candida albicans isolatesen_US
dc.typeArticleen_US
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1034/j.1600-0714.2000.290503.xen_US
dc.identifier.pmid10801037-
dc.identifier.scopuseid_2-s2.0-0034055628en_US
dc.identifier.hkuros48790-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034055628&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume29en_US
dc.identifier.issue5en_US
dc.identifier.spage206en_US
dc.identifier.epage213en_US
dc.identifier.isiWOS:000086714600003-
dc.publisher.placeDenmarken_US
dc.identifier.scopusauthoridEgusa, H=6602170721en_US
dc.identifier.scopusauthoridEllepola, ANB=6604060863en_US
dc.identifier.scopusauthoridNikawa, H=7006724162en_US
dc.identifier.scopusauthoridHamada, T=7401759268en_US
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats