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Article: Vascular endothelial growth pattern of endochondral bone graft in the presence of demineralized intramembranous bone matrix - Quantitative analysis

TitleVascular endothelial growth pattern of endochondral bone graft in the presence of demineralized intramembranous bone matrix - Quantitative analysis
Authors
KeywordsAngiogenesis
Bone induction
Composite endochondral bone/demineralized intramembranous bone matrix
Demineralized bone matrix
EN 7/44
Endochondral bone
Intramembranous bone
Issue Date2000
PublisherAllen Press Inc. The Journal's web site is located at http://cpcj.allenpress.com
Citation
Cleft Palate-Craniofacial Journal, 2000, v. 37 n. 4, p. 385-394 How to Cite?
AbstractObjective: To determine the timely ingrowth of new blood vessels of composite endochondral (EC) bone and demineralize bone matrix (DBM) prepared from intramembranous (IM) origin (EC-DBM(IM)) and to compare it with EC bone graft alone. Design: Thirty-two rabbits with 32 critical-size (10 x 5 mm), full-thickness bony defects in rabbit parietal bone were divided into two groups: composite EC-DBM(IM) group - implanted with composite autogenous EC bone and DBM(IM); EC bone group - implanted with EC bone alone. Two rabbits from each group were sacrificed 1, 2, 3, 4, 5, 6, 7, and 14 days after grafting. Neovascularization was assessed by immunohistochemical staining with antihuman angiogenesis-related endothelial cell antibodies (EN 7/44). Quantitative analysis of neovascularization, represented by percentage area of positive immunohistochemical staining, was performed on 320 sections of the experimental groups by a computer-assisted image analyzer. Results: Positive immunohistochemical staining was first identified on day 2 post grafting for the composite EC-DBM(IM) group in comparison with day 4 in the EC bone graft group. The composite EC-DBM(IM) bone graft group showed earlier and almost 100% more neovascularization when compared with the EC bone graft group. Conclusion: DBM(IM) enhances healing and integration of EC bone graft by enhancing vascularization as well as increasing the amount of new blood vessels formed. In clinical cases in which EC autogenous bone is used to graft a large defect such as in cleft palate and craniofacial surgery, DBM(IM) should allow better integration and healing of the EC bone graft to the host bone.
Persistent Identifierhttp://hdl.handle.net/10722/154088
ISSN
2010 Impact Factor: 0.77
2015 SCImago Journal Rankings: 0.685
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChow, KMCen_HK
dc.contributor.authorRabie, ABMen_HK
dc.date.accessioned2012-08-08T08:23:12Z-
dc.date.available2012-08-08T08:23:12Z-
dc.date.issued2000en_HK
dc.identifier.citationCleft Palate-Craniofacial Journal, 2000, v. 37 n. 4, p. 385-394en_HK
dc.identifier.issn1055-6656en_HK
dc.identifier.urihttp://hdl.handle.net/10722/154088-
dc.description.abstractObjective: To determine the timely ingrowth of new blood vessels of composite endochondral (EC) bone and demineralize bone matrix (DBM) prepared from intramembranous (IM) origin (EC-DBM(IM)) and to compare it with EC bone graft alone. Design: Thirty-two rabbits with 32 critical-size (10 x 5 mm), full-thickness bony defects in rabbit parietal bone were divided into two groups: composite EC-DBM(IM) group - implanted with composite autogenous EC bone and DBM(IM); EC bone group - implanted with EC bone alone. Two rabbits from each group were sacrificed 1, 2, 3, 4, 5, 6, 7, and 14 days after grafting. Neovascularization was assessed by immunohistochemical staining with antihuman angiogenesis-related endothelial cell antibodies (EN 7/44). Quantitative analysis of neovascularization, represented by percentage area of positive immunohistochemical staining, was performed on 320 sections of the experimental groups by a computer-assisted image analyzer. Results: Positive immunohistochemical staining was first identified on day 2 post grafting for the composite EC-DBM(IM) group in comparison with day 4 in the EC bone graft group. The composite EC-DBM(IM) bone graft group showed earlier and almost 100% more neovascularization when compared with the EC bone graft group. Conclusion: DBM(IM) enhances healing and integration of EC bone graft by enhancing vascularization as well as increasing the amount of new blood vessels formed. In clinical cases in which EC autogenous bone is used to graft a large defect such as in cleft palate and craniofacial surgery, DBM(IM) should allow better integration and healing of the EC bone graft to the host bone.en_HK
dc.languageengen_US
dc.publisherAllen Press Inc. The Journal's web site is located at http://cpcj.allenpress.comen_HK
dc.relation.ispartofCleft Palate-Craniofacial Journalen_HK
dc.subjectAngiogenesisen_HK
dc.subjectBone inductionen_HK
dc.subjectComposite endochondral bone/demineralized intramembranous bone matrixen_HK
dc.subjectDemineralized bone matrixen_HK
dc.subjectEN 7/44en_HK
dc.subjectEndochondral boneen_HK
dc.subjectIntramembranous boneen_HK
dc.subject.meshAnimalsen_US
dc.subject.meshBone Matrixen_US
dc.subject.meshBone Substitutes - Therapeutic Useen_US
dc.subject.meshBone Transplantation - Methods - Pathology - Physiologyen_US
dc.subject.meshColoring Agents - Diagnostic Useen_US
dc.subject.meshEndothelium, Vascular - Growth & Developmenten_US
dc.subject.meshImage Processing, Computer-Assisteden_US
dc.subject.meshImmunoenzyme Techniquesen_US
dc.subject.meshNeovascularization, Physiologic - Physiologyen_US
dc.subject.meshOsteogenesis - Physiologyen_US
dc.subject.meshParietal Bone - Surgeryen_US
dc.subject.meshRabbitsen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTransplantation, Autologousen_US
dc.subject.meshWound Healingen_US
dc.titleVascular endothelial growth pattern of endochondral bone graft in the presence of demineralized intramembranous bone matrix - Quantitative analysisen_HK
dc.typeArticleen_HK
dc.identifier.emailRabie, ABM: rabie@hku.hken_HK
dc.identifier.authorityRabie, ABM=rp00029en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid10912718-
dc.identifier.scopuseid_2-s2.0-0033938565en_HK
dc.identifier.hkuros48476-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033938565&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume37en_HK
dc.identifier.issue4en_HK
dc.identifier.spage385en_HK
dc.identifier.epage394en_HK
dc.identifier.isiWOS:000088074300009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChow, KMC=7202178189en_HK
dc.identifier.scopusauthoridRabie, ABM=7007172734en_HK

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