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Article: Actinobacillus actinomycetemcomitans in Chinese adults: Serotype distribution and analysis of the leukotoxin gene promoter locus

TitleActinobacillus actinomycetemcomitans in Chinese adults: Serotype distribution and analysis of the leukotoxin gene promoter locus
Authors
Issue Date1999
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE
Citation
Journal Of Clinical Periodontology, 1999, v. 26 n. 8, p. 505-510 How to Cite?
AbstractThe aim of the present study was to examine the Actinobacillus actinomycetemcomitans carrier rate in Chinese subjects, and to determine serotype distribution, presence of the leukotoxin gene lktA and the structure of the lktA-promoter region. Subgingival microbiological samples were obtained from 31 Chinese subjects with moderate to advanced adult periodontitis, 73 young factory workers, and 81 adult residents of a rural area. Bacterial isolates phenotypically identified as A. actinomycetemcomitans were found in 116 of the 185 subjects (detection frequency over-all: 63%). Presence of the leukotoxin gene lktA was demonstrated for all 115 isolates that could be subcultured. The PCR analysis of the lktA-promoter region showed that none of these strains had the deletion in the promoter region known to enhance expression of lktA. No significant difference in the frequency of A. actinomycetemcomitans could be observed between the subjects of the 3 study groups. Analysis by logistic multiple regression indicated a homogeneous distribution of A. actinomycetemcomitans in the 3 cohorts and a lack of significant influence of subject gender or age. Serotype a was found in 21 subjects, serotype b was found in 9, serotype c in 67 and serotype e in 11 individuals. Serotype d was not detected in any subject. Nontypeable isolates, lacking serotype a, b, c, d, or e antigens, were found in 9 individuals. A high prevalence irrespective of gender, age, and cohort suggests that A. actinomycetemcomitans is a common constituent of the normal flora in the Chinese subjects of this study and suggests differences in the microbiological composition of subgingival plaque may exist for this population group as compared to north American and European populations. © Munksgaard, 1999.
Persistent Identifierhttp://hdl.handle.net/10722/154069
ISSN
2015 Impact Factor: 3.915
2015 SCImago Journal Rankings: 1.848
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMombelli, Aen_US
dc.contributor.authorGmür, Ren_US
dc.contributor.authorLang, NPen_US
dc.contributor.authorCorbet, Een_US
dc.contributor.authorFrey, Jen_US
dc.date.accessioned2012-08-08T08:23:06Z-
dc.date.available2012-08-08T08:23:06Z-
dc.date.issued1999en_US
dc.identifier.citationJournal Of Clinical Periodontology, 1999, v. 26 n. 8, p. 505-510en_US
dc.identifier.issn0303-6979en_US
dc.identifier.urihttp://hdl.handle.net/10722/154069-
dc.description.abstractThe aim of the present study was to examine the Actinobacillus actinomycetemcomitans carrier rate in Chinese subjects, and to determine serotype distribution, presence of the leukotoxin gene lktA and the structure of the lktA-promoter region. Subgingival microbiological samples were obtained from 31 Chinese subjects with moderate to advanced adult periodontitis, 73 young factory workers, and 81 adult residents of a rural area. Bacterial isolates phenotypically identified as A. actinomycetemcomitans were found in 116 of the 185 subjects (detection frequency over-all: 63%). Presence of the leukotoxin gene lktA was demonstrated for all 115 isolates that could be subcultured. The PCR analysis of the lktA-promoter region showed that none of these strains had the deletion in the promoter region known to enhance expression of lktA. No significant difference in the frequency of A. actinomycetemcomitans could be observed between the subjects of the 3 study groups. Analysis by logistic multiple regression indicated a homogeneous distribution of A. actinomycetemcomitans in the 3 cohorts and a lack of significant influence of subject gender or age. Serotype a was found in 21 subjects, serotype b was found in 9, serotype c in 67 and serotype e in 11 individuals. Serotype d was not detected in any subject. Nontypeable isolates, lacking serotype a, b, c, d, or e antigens, were found in 9 individuals. A high prevalence irrespective of gender, age, and cohort suggests that A. actinomycetemcomitans is a common constituent of the normal flora in the Chinese subjects of this study and suggests differences in the microbiological composition of subgingival plaque may exist for this population group as compared to north American and European populations. © Munksgaard, 1999.en_US
dc.languageengen_US
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPEen_US
dc.relation.ispartofJournal of Clinical Periodontologyen_US
dc.subject.meshActinobacillus Actinomycetemcomitans - Classification - Geneticsen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAge Distributionen_US
dc.subject.meshAgeden_US
dc.subject.meshAsian Continental Ancestry Group - Geneticsen_US
dc.subject.meshBacterial Proteinsen_US
dc.subject.meshCarrier State - Ethnology - Microbiologyen_US
dc.subject.meshChina - Epidemiologyen_US
dc.subject.meshDental Plaque - Ethnology - Microbiologyen_US
dc.subject.meshExotoxins - Geneticsen_US
dc.subject.meshFemaleen_US
dc.subject.meshGenes, Bacterialen_US
dc.subject.meshHemolysin Proteins - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPeriodontal Indexen_US
dc.subject.meshPeriodontitis - Ethnology - Microbiologyen_US
dc.subject.meshPrevalenceen_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshSerotypingen_US
dc.subject.meshSex Distributionen_US
dc.titleActinobacillus actinomycetemcomitans in Chinese adults: Serotype distribution and analysis of the leukotoxin gene promoter locusen_US
dc.typeArticleen_US
dc.identifier.emailLang, NP:nplang@hkucc.hku.hken_US
dc.identifier.emailCorbet, E:efcorbet@hku.hken_US
dc.identifier.authorityLang, NP=rp00031en_US
dc.identifier.authorityCorbet, E=rp00005en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1034/j.1600-051X.1999.260803.x-
dc.identifier.pmid10450810en_US
dc.identifier.scopuseid_2-s2.0-0033174294en_US
dc.identifier.hkuros41758-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033174294&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume26en_US
dc.identifier.issue8en_US
dc.identifier.spage505en_US
dc.identifier.epage510en_US
dc.identifier.isiWOS:000081598900003-
dc.publisher.placeDenmarken_US
dc.identifier.scopusauthoridMombelli, A=7006180872en_US
dc.identifier.scopusauthoridGmür, R=7003317290en_US
dc.identifier.scopusauthoridLang, NP=7201577367en_US
dc.identifier.scopusauthoridCorbet, E=35609873200en_US
dc.identifier.scopusauthoridFrey, J=7202811675en_US

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