File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: In vitro cytotoxicity of orthodontic bonding resins on human oral fibroblasts.

TitleIn vitro cytotoxicity of orthodontic bonding resins on human oral fibroblasts.
Authors
Issue Date1999
PublisherMosby, Inc. The Journal's web site is located at http://www.elsevier.com/locate/ajodo
Citation
American Journal Of Orthodontics And Dentofacial Orthopedics : Official Publication Of The American Association Of Orthodontists, Its Constituent Societies, And The American Board Of Orthodontics, 1999, v. 116 n. 2, p. 132-138 How to Cite?
AbstractPolymerization of bonding resins is compromised by atmospheric oxygen, giving rise to a layer of low molecular weight chemical species commonly known as the oxygen inhibited layer. The aim of this study was to evaluate the cytotoxic effect of this layer on primary cultures of human oral fibroblast. The cytotoxic effect related to the modes of polymerization of seven commercially available orthodontic bonding resins was also evaluated statistically. Each material was polymerized into 12 resin disks of standardized dimensions. Half of them were washed with 99% acetone to remove the oxygen inhibited layer. In duplicates, human oral fibroblasts were exposed to the intact and washed resin disks in tissue culture inserts. Cell viability was assessed by tetrazolium bromide reduction assay (MTT) 1, 3, and 6 days after exposure. Glass disks served as controls. ANOVA was used to test for statistical significance. Overall, the presence of an oxygen inhibited layer renders bonding resins 33% more cytotoxic (P <.01, F = 11.83, DF = 1). Light-cured and chemically cured 2-pastes materials had their mean cytotoxicities approximating their inert controls over 6 days. In chemically cured liquid-paste materials, the viability of human oral fibroblasts was only 37% (P <.001, F = 26.4, DF = 2) comparing to the control, 64% on day 1, 30% on day 3 and 14% on day 6. This suggested that the oxygen inhibited layer formed on the surface of bonding resins is an important cytotoxic source in vitro. Chemically cured liquid-paste materials were more cytotoxic than light-cured and chemically cured 2-paste materials. Further investigation into the influence of the modes of polymerization on materials' toxicodynamic effect is warranted to verify its clinical implication.
Persistent Identifierhttp://hdl.handle.net/10722/154068
ISSN
2015 Impact Factor: 1.69
2015 SCImago Journal Rankings: 1.249
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTang, ATen_US
dc.contributor.authorLiu, Yen_US
dc.contributor.authorBjörkman, Len_US
dc.contributor.authorEkstrand, Jen_US
dc.date.accessioned2012-08-08T08:23:06Z-
dc.date.available2012-08-08T08:23:06Z-
dc.date.issued1999en_US
dc.identifier.citationAmerican Journal Of Orthodontics And Dentofacial Orthopedics : Official Publication Of The American Association Of Orthodontists, Its Constituent Societies, And The American Board Of Orthodontics, 1999, v. 116 n. 2, p. 132-138en_US
dc.identifier.issn0889-5406en_US
dc.identifier.urihttp://hdl.handle.net/10722/154068-
dc.description.abstractPolymerization of bonding resins is compromised by atmospheric oxygen, giving rise to a layer of low molecular weight chemical species commonly known as the oxygen inhibited layer. The aim of this study was to evaluate the cytotoxic effect of this layer on primary cultures of human oral fibroblast. The cytotoxic effect related to the modes of polymerization of seven commercially available orthodontic bonding resins was also evaluated statistically. Each material was polymerized into 12 resin disks of standardized dimensions. Half of them were washed with 99% acetone to remove the oxygen inhibited layer. In duplicates, human oral fibroblasts were exposed to the intact and washed resin disks in tissue culture inserts. Cell viability was assessed by tetrazolium bromide reduction assay (MTT) 1, 3, and 6 days after exposure. Glass disks served as controls. ANOVA was used to test for statistical significance. Overall, the presence of an oxygen inhibited layer renders bonding resins 33% more cytotoxic (P <.01, F = 11.83, DF = 1). Light-cured and chemically cured 2-pastes materials had their mean cytotoxicities approximating their inert controls over 6 days. In chemically cured liquid-paste materials, the viability of human oral fibroblasts was only 37% (P <.001, F = 26.4, DF = 2) comparing to the control, 64% on day 1, 30% on day 3 and 14% on day 6. This suggested that the oxygen inhibited layer formed on the surface of bonding resins is an important cytotoxic source in vitro. Chemically cured liquid-paste materials were more cytotoxic than light-cured and chemically cured 2-paste materials. Further investigation into the influence of the modes of polymerization on materials' toxicodynamic effect is warranted to verify its clinical implication.en_US
dc.languageengen_US
dc.publisherMosby, Inc. The Journal's web site is located at http://www.elsevier.com/locate/ajodoen_US
dc.relation.ispartofAmerican journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodonticsen_US
dc.subject.meshAnalysis Of Varianceen_US
dc.subject.meshBisphenol A-Glycidyl Methacrylate - Chemistry - Toxicityen_US
dc.subject.meshCell Survival - Drug Effectsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshDental Bondingen_US
dc.subject.meshFibroblasts - Drug Effectsen_US
dc.subject.meshGingiva - Cytology - Drug Effectsen_US
dc.subject.meshHumansen_US
dc.subject.meshMolecular Weighten_US
dc.subject.meshReactive Oxygen Speciesen_US
dc.subject.meshSurface Propertiesen_US
dc.subject.meshTime Factorsen_US
dc.titleIn vitro cytotoxicity of orthodontic bonding resins on human oral fibroblasts.en_US
dc.typeArticleen_US
dc.identifier.emailTang, AT:athtang@hku.hken_US
dc.identifier.authorityTang, AT=rp00054en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid10434085-
dc.identifier.scopuseid_2-s2.0-0033172831en_US
dc.identifier.volume116en_US
dc.identifier.issue2en_US
dc.identifier.spage132en_US
dc.identifier.epage138en_US
dc.identifier.isiWOS:000081967200007-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridTang, AT=7201843899en_US
dc.identifier.scopusauthoridLiu, Y=27169762800en_US
dc.identifier.scopusauthoridBjörkman, L=7003954336en_US
dc.identifier.scopusauthoridEkstrand, J=7005484046en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats