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Article: Cytotoxicity tests of in situ polymerized resins: Methodological comparisons and introduction of a tissue culture insert as a testing device

TitleCytotoxicity tests of in situ polymerized resins: Methodological comparisons and introduction of a tissue culture insert as a testing device
Authors
Issue Date1999
Citation
Journal Of Biomedical Materials Research, 1999, v. 45 n. 3, p. 214-222 How to Cite?
AbstractThe in vitro cytotoxic potential of six commonly used methacrylate polymers was evaluated using human oral fibroblast cultures with different cell-material contact systems. A tissue culture insert was introduced to test resin-released components. Both acute and delayed cytotoxic effects of resin were quantified by cellular enzymatic and DNA synthetic activities of fibroblasts over a 6-day exposure period. Resin toxicity was material- dependent. Statistical analysis showed that the experimental conditions significantly contributed to the overall toxicity and the cytotoxicity pattern for a given material. DNA synthesis activity of human oral fibroblasts assayed by 3H-thymidine incorporation was more sensitive to resins than cellular enzyme activity, as determined by tetrazolium bromide reduction. However, extended exposure increased the cytotoxicity of all resins, as measured by tetrazolium bromide reduction, which seemed to be a better indicator of the development of resin toxicity than 3H-thymidine incorporation. Removal of the oxygen inhibition layer on resin specimens partially enhanced cell viability, indicating that this surface layer together with other unknown factors contributed to resin toxicity. | The in vitro cytotoxic potential of six commonly used methacrylate polymers was evaluated using human oral fibroblast cultures with different cell-material contact systems. A tissue culture insert was introduced to test resin-released components. Both acute and delayed cytotoxic effects of resin were quantified by cellular enzymatic and DNA synthetic activities of fibroblasts over a 6-day exposure period. Resin toxicity was material-dependent. Statistical analysis showed that the experimental conditions significantly contributed to the overall toxicity and the cytotoxicity pattern for a given material. DNA synthesis activity of human oral fibroblasts assayed by 3H-thymidine incorporation was more sensitive to resins than cellular enzyme activity, as determined by tetrazolium bromide reduction. However, extended exposure increased the cytotoxicity of all resins, as measured by tetrazolium bromide reduction, which seemed to be a better indicator of the development of resin toxicity than 3H-thymidine incorporation. Removal of the oxygen inhibition layer on resin specimens partially enhanced cell viability, indicating that this surface layer together with other unknown factors contributed to resin toxicity.
Persistent Identifierhttp://hdl.handle.net/10722/154055
ISSN
2004 Impact Factor: 3.652
2006 SCImago Journal Rankings: 0.474
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTang, ATHen_US
dc.contributor.authorLi, Jen_US
dc.contributor.authorEkstrand, Jen_US
dc.contributor.authorLiu, Yen_US
dc.date.accessioned2012-08-08T08:23:02Z-
dc.date.available2012-08-08T08:23:02Z-
dc.date.issued1999en_US
dc.identifier.citationJournal Of Biomedical Materials Research, 1999, v. 45 n. 3, p. 214-222en_US
dc.identifier.issn0021-9304en_US
dc.identifier.urihttp://hdl.handle.net/10722/154055-
dc.description.abstractThe in vitro cytotoxic potential of six commonly used methacrylate polymers was evaluated using human oral fibroblast cultures with different cell-material contact systems. A tissue culture insert was introduced to test resin-released components. Both acute and delayed cytotoxic effects of resin were quantified by cellular enzymatic and DNA synthetic activities of fibroblasts over a 6-day exposure period. Resin toxicity was material- dependent. Statistical analysis showed that the experimental conditions significantly contributed to the overall toxicity and the cytotoxicity pattern for a given material. DNA synthesis activity of human oral fibroblasts assayed by 3H-thymidine incorporation was more sensitive to resins than cellular enzyme activity, as determined by tetrazolium bromide reduction. However, extended exposure increased the cytotoxicity of all resins, as measured by tetrazolium bromide reduction, which seemed to be a better indicator of the development of resin toxicity than 3H-thymidine incorporation. Removal of the oxygen inhibition layer on resin specimens partially enhanced cell viability, indicating that this surface layer together with other unknown factors contributed to resin toxicity. | The in vitro cytotoxic potential of six commonly used methacrylate polymers was evaluated using human oral fibroblast cultures with different cell-material contact systems. A tissue culture insert was introduced to test resin-released components. Both acute and delayed cytotoxic effects of resin were quantified by cellular enzymatic and DNA synthetic activities of fibroblasts over a 6-day exposure period. Resin toxicity was material-dependent. Statistical analysis showed that the experimental conditions significantly contributed to the overall toxicity and the cytotoxicity pattern for a given material. DNA synthesis activity of human oral fibroblasts assayed by 3H-thymidine incorporation was more sensitive to resins than cellular enzyme activity, as determined by tetrazolium bromide reduction. However, extended exposure increased the cytotoxicity of all resins, as measured by tetrazolium bromide reduction, which seemed to be a better indicator of the development of resin toxicity than 3H-thymidine incorporation. Removal of the oxygen inhibition layer on resin specimens partially enhanced cell viability, indicating that this surface layer together with other unknown factors contributed to resin toxicity.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Biomedical Materials Researchen_US
dc.subject.meshCell Survival - Drug Effectsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshColoring Agentsen_US
dc.subject.meshCulture Techniquesen_US
dc.subject.meshDna - Biosynthesisen_US
dc.subject.meshFibroblastsen_US
dc.subject.meshGingiva - Pathologyen_US
dc.subject.meshHumansen_US
dc.subject.meshIndicators And Reagentsen_US
dc.subject.meshMaleen_US
dc.subject.meshMaterials Testing - Methodsen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshNeutral Reden_US
dc.subject.meshOxidation-Reductionen_US
dc.subject.meshPolymers - Toxicityen_US
dc.subject.meshResins, Plant - Toxicityen_US
dc.subject.meshSurface Propertiesen_US
dc.subject.meshTetrazolium Saltsen_US
dc.subject.meshThymidine - Metabolismen_US
dc.titleCytotoxicity tests of in situ polymerized resins: Methodological comparisons and introduction of a tissue culture insert as a testing deviceen_US
dc.typeArticleen_US
dc.identifier.emailTang, ATH:athtang@hku.hken_US
dc.identifier.authorityTang, ATH=rp00054en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/(SICI)1097-4636(19990605)45:3<214::AID-JBM9>3.0.CO;2-Yen_US
dc.identifier.pmid10397979-
dc.identifier.scopuseid_2-s2.0-0032945650en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032945650&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume45en_US
dc.identifier.issue3en_US
dc.identifier.spage214en_US
dc.identifier.epage222en_US
dc.identifier.isiWOS:000079565900009-
dc.identifier.scopusauthoridTang, ATH=7201843899en_US
dc.identifier.scopusauthoridLi, J=7410061153en_US
dc.identifier.scopusauthoridEkstrand, J=7005484046en_US
dc.identifier.scopusauthoridLiu, Y=27169762800en_US

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