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Article: Adhesion of oral Candida species to human buccal epithelial cells following brief exposure to nystatin

TitleAdhesion of oral Candida species to human buccal epithelial cells following brief exposure to nystatin
Authors
Issue Date1999
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/OMI
Citation
Oral Microbiology And Immunology, 1999, v. 14 n. 6, p. 358-363 How to Cite?
AbstractOpportunistic oral infections caused by Candida albicans and non-albicans Candida species are particularly common in compromised patients. Nystatin, which belongs to the polyene group of antimycotics, is frequently used as a topical agent in the treatment of oro-pharyngeal candidosis. It is recognized that due to the delivery mode of nystatin (i.e. topical, intermittent), as well as the cleansing effect of saliva within the oral environment, the yeasts undergo a relatively brief exposure to this drug during treatment. Nevertheless, there is a sparsity of data on the effect of such brief exposure to nystatin on the pathogenic attributes of Candida such as their adherence to host surfaces. The adhesion of microbes to host mucosal surfaces is a major determinant of successful colonization and infection. Thus the main aim of our investigation was to compare the in vitro adhesion of 30 oral isolates of Candida belonging to six different species (comprising Candida albicans, Candida tropicalis, Candida glabrata, Candida guilliermondii, Candida krusei and Candida parapsilosis) to human buccal epithelial cells, following their brief exposure (1 h) to minimum inhibitory concentration of nystatin, and subsequent removal of the drug. The adhesion of these isolates to buccal epithelial cells was assessed by a previously described adhesion assay. Compared with the controls, there was a significant reduction in buccal epithelial cell adhesion of all six Candida species after drug exposure (54%-68%). However the adhesion of C. albicans isolates was the least affected by nystatin exposure, which was significantly different from that of the non-albicans species. These findings imply that sub-therapeutic levels of nystatin, which are likely to persist in the oral cavity during dosing intervals, may also be beneficial, as they inhibit candidal colonization. The significant difference in nystatin-induced suppression of adhesion between C. albicans and the non-albicans species investigated is a further testimonial for the pre-eminent virulence of the former species.
Persistent Identifierhttp://hdl.handle.net/10722/154053
ISSN
2011 Impact Factor: 2.807
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorEllepola, ANBen_US
dc.contributor.authorPanagoda, GJen_US
dc.contributor.authorSamaranayake, LPen_US
dc.date.accessioned2012-08-08T08:23:01Z-
dc.date.available2012-08-08T08:23:01Z-
dc.date.issued1999en_US
dc.identifier.citationOral Microbiology And Immunology, 1999, v. 14 n. 6, p. 358-363en_US
dc.identifier.issn0902-0055en_US
dc.identifier.urihttp://hdl.handle.net/10722/154053-
dc.description.abstractOpportunistic oral infections caused by Candida albicans and non-albicans Candida species are particularly common in compromised patients. Nystatin, which belongs to the polyene group of antimycotics, is frequently used as a topical agent in the treatment of oro-pharyngeal candidosis. It is recognized that due to the delivery mode of nystatin (i.e. topical, intermittent), as well as the cleansing effect of saliva within the oral environment, the yeasts undergo a relatively brief exposure to this drug during treatment. Nevertheless, there is a sparsity of data on the effect of such brief exposure to nystatin on the pathogenic attributes of Candida such as their adherence to host surfaces. The adhesion of microbes to host mucosal surfaces is a major determinant of successful colonization and infection. Thus the main aim of our investigation was to compare the in vitro adhesion of 30 oral isolates of Candida belonging to six different species (comprising Candida albicans, Candida tropicalis, Candida glabrata, Candida guilliermondii, Candida krusei and Candida parapsilosis) to human buccal epithelial cells, following their brief exposure (1 h) to minimum inhibitory concentration of nystatin, and subsequent removal of the drug. The adhesion of these isolates to buccal epithelial cells was assessed by a previously described adhesion assay. Compared with the controls, there was a significant reduction in buccal epithelial cell adhesion of all six Candida species after drug exposure (54%-68%). However the adhesion of C. albicans isolates was the least affected by nystatin exposure, which was significantly different from that of the non-albicans species. These findings imply that sub-therapeutic levels of nystatin, which are likely to persist in the oral cavity during dosing intervals, may also be beneficial, as they inhibit candidal colonization. The significant difference in nystatin-induced suppression of adhesion between C. albicans and the non-albicans species investigated is a further testimonial for the pre-eminent virulence of the former species.en_US
dc.languageengen_US
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/OMIen_US
dc.relation.ispartofOral Microbiology and Immunologyen_US
dc.subject.meshAntifungal Agents - Administration & Dosageen_US
dc.subject.meshCandida - Drug Effects - Physiologyen_US
dc.subject.meshCandida Albicans - Drug Effects - Pathogenicityen_US
dc.subject.meshCell Adhesion - Drug Effectsen_US
dc.subject.meshColony Count, Microbialen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshEpithelial Cells - Microbiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMicrobial Sensitivity Testsen_US
dc.subject.meshMouth - Cytology - Microbiologyen_US
dc.subject.meshNystatin - Administration & Dosageen_US
dc.subject.meshSpecies Specificityen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshVirulenceen_US
dc.titleAdhesion of oral Candida species to human buccal epithelial cells following brief exposure to nystatinen_US
dc.typeArticleen_US
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1034/j.1399-302X.1999.140605.xen_US
dc.identifier.pmid10895691-
dc.identifier.scopuseid_2-s2.0-0032721204en_US
dc.identifier.hkuros47499-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032721204&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume14en_US
dc.identifier.issue6en_US
dc.identifier.spage358en_US
dc.identifier.epage363en_US
dc.identifier.isiWOS:000083790600005-
dc.publisher.placeDenmarken_US
dc.identifier.scopusauthoridEllepola, ANB=6604060863en_US
dc.identifier.scopusauthoridPanagoda, GJ=6507149433en_US
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_US

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