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Article: Surface topography and serum concentration affect the appearance of tenascin in human gingival fibroblasts in vitro

TitleSurface topography and serum concentration affect the appearance of tenascin in human gingival fibroblasts in vitro
Authors
Issue Date1998
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexcr
Citation
Experimental Cell Research, 1998, v. 244 n. 2, p. 474-480 How to Cite?
AbstractTenascin is an extracellular matrix glycoprotein which affects cell behavior such as cell migration. This study was undertaken to investigate the time of appearance of tenascin (TN) in human gingival fibroblasts (HGF) and how it was affected by the surface topography of the titanium substratum or by serum concentration in the medium. HGF were cultured for 4 to 24 h and then processed for confocal immunofluorescence microscopy. Very few cells stained positive for TN 4 h after plating, but the number of TN-positive HGF gradually increased between 8 and 18 h after plating. The increase in the rate of the proportion of TN-positive cells on the grooved surface lagged behind that of HGF cultured on the smooth surface. The number of TN-positive cells in medium + 15% serum was significantly greater than that of cells in 5% serum or serum-free medium. The number of TN-positive cells was greater on the smooth titanium surface than on the grooved titanium surface in both 15% serum and 5% serum-containing medium. These findings suggest that TN production by fibroblasts in vitro can be modulated by factors in serum and by the surface topography of the substratum.
Persistent Identifierhttp://hdl.handle.net/10722/154044
ISSN
2015 Impact Factor: 3.378
2015 SCImago Journal Rankings: 1.900
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGoto, Ten_US
dc.contributor.authorBrunette, DMen_US
dc.date.accessioned2012-08-08T08:22:59Z-
dc.date.available2012-08-08T08:22:59Z-
dc.date.issued1998en_US
dc.identifier.citationExperimental Cell Research, 1998, v. 244 n. 2, p. 474-480en_US
dc.identifier.issn0014-4827en_US
dc.identifier.urihttp://hdl.handle.net/10722/154044-
dc.description.abstractTenascin is an extracellular matrix glycoprotein which affects cell behavior such as cell migration. This study was undertaken to investigate the time of appearance of tenascin (TN) in human gingival fibroblasts (HGF) and how it was affected by the surface topography of the titanium substratum or by serum concentration in the medium. HGF were cultured for 4 to 24 h and then processed for confocal immunofluorescence microscopy. Very few cells stained positive for TN 4 h after plating, but the number of TN-positive HGF gradually increased between 8 and 18 h after plating. The increase in the rate of the proportion of TN-positive cells on the grooved surface lagged behind that of HGF cultured on the smooth surface. The number of TN-positive cells in medium + 15% serum was significantly greater than that of cells in 5% serum or serum-free medium. The number of TN-positive cells was greater on the smooth titanium surface than on the grooved titanium surface in both 15% serum and 5% serum-containing medium. These findings suggest that TN production by fibroblasts in vitro can be modulated by factors in serum and by the surface topography of the substratum.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexcren_US
dc.relation.ispartofExperimental Cell Researchen_US
dc.subject.meshBlood Proteins - Pharmacologyen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCulture Media, Conditioneden_US
dc.subject.meshCulture Media, Serum-Free - Pharmacologyen_US
dc.subject.meshFibroblasts - Metabolismen_US
dc.subject.meshFluorescent Antibody Techniqueen_US
dc.subject.meshGingiva - Cytologyen_US
dc.subject.meshHumansen_US
dc.subject.meshStaining And Labelingen_US
dc.subject.meshSurface Propertiesen_US
dc.subject.meshTenascin - Blood - Metabolismen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTitanium - Chemistryen_US
dc.titleSurface topography and serum concentration affect the appearance of tenascin in human gingival fibroblasts in vitroen_US
dc.typeArticleen_US
dc.identifier.emailGoto, T:gototk@hku.hken_US
dc.identifier.authorityGoto, T=rp01434en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/excr.1998.4196en_US
dc.identifier.pmid9806798-
dc.identifier.scopuseid_2-s2.0-0032213626en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032213626&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume244en_US
dc.identifier.issue2en_US
dc.identifier.spage474en_US
dc.identifier.epage480en_US
dc.identifier.isiWOS:000076869200011-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridGoto, T=7403938313en_US
dc.identifier.scopusauthoridBrunette, DM=7005815978en_US

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