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Article: The effect of limited exposure to antimycotics on the relative cell- surface hydrophobicity and the adhesion of oral Candida albicans to buccal epithelial cells

TitleThe effect of limited exposure to antimycotics on the relative cell- surface hydrophobicity and the adhesion of oral Candida albicans to buccal epithelial cells
Authors
Issue Date1998
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio
Citation
Archives Of Oral Biology, 1998, v. 43 n. 11, p. 879-887 How to Cite?
AbstractCandida albicans is the major aetiological agent of oral candidosis. Adhesion to oral mucosal surfaces is considered a prerequisite for its successful colonization and subsequent infection, and its relative cell- surface hydrophobicity (CSH) is a contributory physical force. Thus, the main aim here was to determine the CSH of 10 isolates of oral C. albicans after a short exposure to sublethal concentrations of four antifungal agents and to correlate these findings with their adhesion to human buccal epithelial cells (BEC). The yeasts were exposed to sublethal concentrations of nystatin [x6 minimal inhibitary concentration (MIC)], 5-fluorocytosine (x8 MIC), ketoconazole (x4 MIC) and fluconazole (x4 MIC) for 1 h. The drug was then removed, and the CSH and BEC adhesion assessed by a biphasic aqueous- hydrocarbon assay and a microscopic method, respectively. The mean percentage reductions of CSH after exposure to nystatin, 5-fluorocytosine, ketoconazole and fluconazole were 27.14% (p = 0.01), 9.46% (p = 0.43), 19.47% (p = 0.04) and 6.16% (p = 0.59). Similarly, exposure to all the drugs except 5- fluorocytosine resulted in a significant inhibition of yeast adhesion to BEC, with nystatin eliciting the highest and fluconazole the least inhibition. Further, on regression analysis a strong positive correlation was observed between CSH and adhesion to BEC after limited exposure to 5-fluorocytosine (r = 0.48, p < 0.0001), ketoconazole (r= 0.48, p < 0.0001), fluconazole (r = 0.55, p < 0.0001) as well as in the unexposed controls (r = 0.41, p = 0.001), although nystatin was an exception (r = 0.09, p = 0.44). Taken together, these data elucidate further mechanisms by which antimycotics may operate in vivo to suppress candidal pathogenicity.
Persistent Identifierhttp://hdl.handle.net/10722/154043
ISSN
2015 Impact Factor: 1.733
2015 SCImago Journal Rankings: 0.713
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorEllepola, ANBen_US
dc.contributor.authorSamaranayake, LPen_US
dc.date.accessioned2012-08-08T08:22:58Z-
dc.date.available2012-08-08T08:22:58Z-
dc.date.issued1998en_US
dc.identifier.citationArchives Of Oral Biology, 1998, v. 43 n. 11, p. 879-887en_US
dc.identifier.issn0003-9969en_US
dc.identifier.urihttp://hdl.handle.net/10722/154043-
dc.description.abstractCandida albicans is the major aetiological agent of oral candidosis. Adhesion to oral mucosal surfaces is considered a prerequisite for its successful colonization and subsequent infection, and its relative cell- surface hydrophobicity (CSH) is a contributory physical force. Thus, the main aim here was to determine the CSH of 10 isolates of oral C. albicans after a short exposure to sublethal concentrations of four antifungal agents and to correlate these findings with their adhesion to human buccal epithelial cells (BEC). The yeasts were exposed to sublethal concentrations of nystatin [x6 minimal inhibitary concentration (MIC)], 5-fluorocytosine (x8 MIC), ketoconazole (x4 MIC) and fluconazole (x4 MIC) for 1 h. The drug was then removed, and the CSH and BEC adhesion assessed by a biphasic aqueous- hydrocarbon assay and a microscopic method, respectively. The mean percentage reductions of CSH after exposure to nystatin, 5-fluorocytosine, ketoconazole and fluconazole were 27.14% (p = 0.01), 9.46% (p = 0.43), 19.47% (p = 0.04) and 6.16% (p = 0.59). Similarly, exposure to all the drugs except 5- fluorocytosine resulted in a significant inhibition of yeast adhesion to BEC, with nystatin eliciting the highest and fluconazole the least inhibition. Further, on regression analysis a strong positive correlation was observed between CSH and adhesion to BEC after limited exposure to 5-fluorocytosine (r = 0.48, p < 0.0001), ketoconazole (r= 0.48, p < 0.0001), fluconazole (r = 0.55, p < 0.0001) as well as in the unexposed controls (r = 0.41, p = 0.001), although nystatin was an exception (r = 0.09, p = 0.44). Taken together, these data elucidate further mechanisms by which antimycotics may operate in vivo to suppress candidal pathogenicity.en_US
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbioen_US
dc.relation.ispartofArchives of Oral Biologyen_US
dc.subject.meshAdulten_US
dc.subject.meshAntifungal Agents - Pharmacologyen_US
dc.subject.meshCandida Albicans - Drug Effects - Isolation & Purification - Pathogenicityen_US
dc.subject.meshCell Adhesion - Drug Effectsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCheeken_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshEpithelial Cells - Drug Effects - Microbiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHiv Seropositivity - Microbiologyen_US
dc.subject.meshHiv-1 - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshLinear Modelsen_US
dc.subject.meshMaleen_US
dc.subject.meshMouth - Microbiologyen_US
dc.subject.meshSurface Propertiesen_US
dc.titleThe effect of limited exposure to antimycotics on the relative cell- surface hydrophobicity and the adhesion of oral Candida albicans to buccal epithelial cellsen_US
dc.typeArticleen_US
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0003-9969(98)00064-8en_US
dc.identifier.pmid9821511-
dc.identifier.scopuseid_2-s2.0-0032212349en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032212349&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume43en_US
dc.identifier.issue11en_US
dc.identifier.spage879en_US
dc.identifier.epage887en_US
dc.identifier.isiWOS:000076631600006-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridEllepola, ANB=6604060863en_US
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_US

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