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Article: A simple monoclonal antibody based ELISA for free protein S. Comparison with PEG precipitation

TitleA simple monoclonal antibody based ELISA for free protein S. Comparison with PEG precipitation
Authors
Issue Date1997
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CLH
Citation
Clinical And Laboratory Haematology, 1997, v. 19 n. 2, p. 111-114 How to Cite?
AbstractA simple monoclonal antibody based ELISA for free Protein S. compatible with out existing ELISA for total Protein S has been developed, and its performance compared with the conventional PEG precipitation method of free Protein S assay. The normal range (mean ± 2 SD) was 0.19-0.54 iu/ml free Protein S. The mean intra assay variation was 5.24% and the mean inter assay variation was 5.50%. A total of 102 routine diagnostic samples from patients referred for prothrombotic investigation (six assays for each method), were assayed by PEG precipitation (mean 0.32 iu/ml, SD 10.60), and the monoclonal ELISA (mean 0.34, SD 0.9). Paired t-test analysis of the two data sets indicated no significant difference between them (P< 0.001). In this sample population, there was no significant difference in free Protein S values when assayed by monoclonal based ELISA or by PEG precipitation. The monoclonal assay has proved to be reliable, accurate and precise. The monoclonal ELISA is simpler, quicker and easier to perform in routine use. Data generated is directly comparable to that generated by PEG precipitation. This methodology would be suitable for laboratories currently measuring Protein S by ELISA.
Persistent Identifierhttp://hdl.handle.net/10722/153987
ISSN
2008 Impact Factor: 1.304
2015 SCImago Journal Rankings: 0.654
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMurdock, PJen_US
dc.contributor.authorBrooks, Sen_US
dc.contributor.authorMellars, Gen_US
dc.contributor.authorCheung, Gen_US
dc.contributor.authorJacob, Den_US
dc.contributor.authorOwens, DLen_US
dc.contributor.authorParmar, Men_US
dc.contributor.authorRiddell, Aen_US
dc.date.accessioned2012-08-08T08:22:40Z-
dc.date.available2012-08-08T08:22:40Z-
dc.date.issued1997en_US
dc.identifier.citationClinical And Laboratory Haematology, 1997, v. 19 n. 2, p. 111-114en_US
dc.identifier.issn0141-9854en_US
dc.identifier.urihttp://hdl.handle.net/10722/153987-
dc.description.abstractA simple monoclonal antibody based ELISA for free Protein S. compatible with out existing ELISA for total Protein S has been developed, and its performance compared with the conventional PEG precipitation method of free Protein S assay. The normal range (mean ± 2 SD) was 0.19-0.54 iu/ml free Protein S. The mean intra assay variation was 5.24% and the mean inter assay variation was 5.50%. A total of 102 routine diagnostic samples from patients referred for prothrombotic investigation (six assays for each method), were assayed by PEG precipitation (mean 0.32 iu/ml, SD 10.60), and the monoclonal ELISA (mean 0.34, SD 0.9). Paired t-test analysis of the two data sets indicated no significant difference between them (P< 0.001). In this sample population, there was no significant difference in free Protein S values when assayed by monoclonal based ELISA or by PEG precipitation. The monoclonal assay has proved to be reliable, accurate and precise. The monoclonal ELISA is simpler, quicker and easier to perform in routine use. Data generated is directly comparable to that generated by PEG precipitation. This methodology would be suitable for laboratories currently measuring Protein S by ELISA.en_US
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CLHen_US
dc.relation.ispartofClinical and Laboratory Haematologyen_US
dc.subject.meshAnalysis Of Varianceen_US
dc.subject.meshAntibodies, Monoclonal - Immunology - Metabolismen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assay - Methodsen_US
dc.subject.meshHumansen_US
dc.subject.meshPolyethylene Glycolsen_US
dc.subject.meshPrecipitin Testsen_US
dc.subject.meshProtein S - Analysis - Immunology - Metabolismen_US
dc.subject.meshReproducibility Of Resultsen_US
dc.titleA simple monoclonal antibody based ELISA for free protein S. Comparison with PEG precipitationen_US
dc.typeArticleen_US
dc.identifier.emailCheung, G:spcheung@hkucc.hku.hken_US
dc.identifier.authorityCheung, G=rp00016en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid9218150-
dc.identifier.scopuseid_2-s2.0-0030876753en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030876753&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume19en_US
dc.identifier.issue2en_US
dc.identifier.spage111en_US
dc.identifier.epage114en_US
dc.identifier.isiWOS:A1997XF58900006-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridMurdock, PJ=36740730900en_US
dc.identifier.scopusauthoridBrooks, S=15762115500en_US
dc.identifier.scopusauthoridMellars, G=17635561600en_US
dc.identifier.scopusauthoridCheung, G=7005809531en_US
dc.identifier.scopusauthoridJacob, D=7102265171en_US
dc.identifier.scopusauthoridOwens, DL=7401549963en_US
dc.identifier.scopusauthoridParmar, M=17636044400en_US
dc.identifier.scopusauthoridRiddell, A=7006168802en_US

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