File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: The role of saliva and serum in Candida albicans biofilm formation on denture acrylic surfaces

TitleThe role of saliva and serum in Candida albicans biofilm formation on denture acrylic surfaces
Authors
KeywordsBiofilm
Candida Albicans
Fibronectin
Mannan-Binding Protein
Saliva
Serum
Issue Date1996
PublisherCo-Action Publishing. The Journal's web site is located at http://www.microbecolhealthdis.net/index.php/mehd/
Citation
Microbial Ecology in Health and Disease, 1996, v. 9 n. 1, p. 35-48 How to Cite?
AbstractThe long term effect of either a salivary or a serum pellicle on Candida albicans biofilm formation on denture acrylic surfaces was investigated both by quantifying the ATP (adenosine triphosphate) content of the resultant biofilms and by scanning electron microscopy. When the biofilm formation on saliva-coated acrylic strips was examined, the yeasts initially colonised this surface at a slower rate than the controls although with increasing incubation time, at 72 h, the ATP content was almost ten-fold higher than the protein-free control strips. Ultrastructural studies revealed this to be due to cell aggregation and hyphal emergence, phenomena not observed in the controls. As compared with the control strips, biofilm activity of the serum-coated strips was almost 100-fold greater within 48 h incubation, and scanning electron microscopy revealed multilayer blastospore-blastospore co-adhesion, germ tube, hyphal and pseudohyphal emergence and blastospore-hyphal coadherence. Further immunocytochemical observation revealed that concanavalin-A binding material and fibronectin were involved in biofilm formation on both saliva and serum coated specimens and, in addition, mannan-binding protein and protein-A binding material also contributed to the biofilm formation on serum coated specimens.
Persistent Identifierhttp://hdl.handle.net/10722/153961
ISSN
1998 Impact Factor: 0.605
2012 SCImago Journal Rankings: 0.281
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNikawa, Hen_US
dc.contributor.authorNishimura, Hen_US
dc.contributor.authorYamamoto, Ten_US
dc.contributor.authorHamada, Ten_US
dc.contributor.authorSamaranayake, LPen_US
dc.date.accessioned2012-08-08T08:22:31Z-
dc.date.available2012-08-08T08:22:31Z-
dc.date.issued1996en_US
dc.identifier.citationMicrobial Ecology in Health and Disease, 1996, v. 9 n. 1, p. 35-48en_US
dc.identifier.issn0891-060Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/153961-
dc.description.abstractThe long term effect of either a salivary or a serum pellicle on Candida albicans biofilm formation on denture acrylic surfaces was investigated both by quantifying the ATP (adenosine triphosphate) content of the resultant biofilms and by scanning electron microscopy. When the biofilm formation on saliva-coated acrylic strips was examined, the yeasts initially colonised this surface at a slower rate than the controls although with increasing incubation time, at 72 h, the ATP content was almost ten-fold higher than the protein-free control strips. Ultrastructural studies revealed this to be due to cell aggregation and hyphal emergence, phenomena not observed in the controls. As compared with the control strips, biofilm activity of the serum-coated strips was almost 100-fold greater within 48 h incubation, and scanning electron microscopy revealed multilayer blastospore-blastospore co-adhesion, germ tube, hyphal and pseudohyphal emergence and blastospore-hyphal coadherence. Further immunocytochemical observation revealed that concanavalin-A binding material and fibronectin were involved in biofilm formation on both saliva and serum coated specimens and, in addition, mannan-binding protein and protein-A binding material also contributed to the biofilm formation on serum coated specimens.en_US
dc.languageengen_US
dc.publisherCo-Action Publishing. The Journal's web site is located at http://www.microbecolhealthdis.net/index.php/mehd/en_US
dc.relation.ispartofMicrobial Ecology in Health and Diseaseen_US
dc.subjectBiofilmen_US
dc.subjectCandida Albicansen_US
dc.subjectFibronectinen_US
dc.subjectMannan-Binding Proteinen_US
dc.subjectSalivaen_US
dc.subjectSerumen_US
dc.titleThe role of saliva and serum in Candida albicans biofilm formation on denture acrylic surfacesen_US
dc.typeArticleen_US
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.scopuseid_2-s2.0-0030019007en_US
dc.identifier.hkuros13944-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030019007&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume9en_US
dc.identifier.issue1en_US
dc.identifier.spage35en_US
dc.identifier.epage48en_US
dc.identifier.isiWOS:A1996TY28400005-
dc.publisher.placeSwedenen_US
dc.identifier.scopusauthoridNikawa, H=7006724162en_US
dc.identifier.scopusauthoridNishimura, H=7402172631en_US
dc.identifier.scopusauthoridYamamoto, T=9533090600en_US
dc.identifier.scopusauthoridHamada, T=7401759268en_US
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats