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Article: The effect of oral commensal bacteria on candidal adhesion to human buccal epithelial cells in vitro

TitleThe effect of oral commensal bacteria on candidal adhesion to human buccal epithelial cells in vitro
Authors
Issue Date1996
PublisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org
Citation
Journal Of Medical Microbiology, 1996, v. 45 n. 3, p. 179-185 How to Cite?
AbstractThe effect of Streptococcus sanguis, S. salivarius, Escherichia coli and Porphyromonas gingivalis on the adhesion of Candida albicans and C. krusei to human buccal epithelial cells (BEC) was investigated with a modified membrane filter system. The filters (12 μm diameter pores) acted as a support for the BEC which were pre-exposed to known concentrations of bacterial suspensions (for 45 min-1 h), and then re-incubated with standardised concentrations of yeast suspensions for various periods. The BEC with adherent yeasts were then transferred on to a glass slide, gram-stained and counted by light microscopy. Three of the four bacterial species significantly suppressed adhesion of C. albicans to BEC; S. sanguis had no effect. Both S. sanguis and S. salivarius suppressed adhesion of C. krusei to BEC pre-exposed to three different bacterial concentrations, although variable results were obtained with P. gingivalis and E. coli. Significant differences in the relative adhesion of C. albicans and C. krusei to BEC were also recorded. These results indicate that the adhesion of yeasts to BEC is modulated both by the composition and the quantity of the pre-existing bacterial flora on the BEC.
Persistent Identifierhttp://hdl.handle.net/10722/153957
ISSN
2015 Impact Factor: 2.269
2015 SCImago Journal Rankings: 1.060
References

 

DC FieldValueLanguage
dc.contributor.authorNair, RGen_US
dc.contributor.authorSamaranayake, LPen_US
dc.date.accessioned2012-08-08T08:22:29Z-
dc.date.available2012-08-08T08:22:29Z-
dc.date.issued1996en_US
dc.identifier.citationJournal Of Medical Microbiology, 1996, v. 45 n. 3, p. 179-185en_US
dc.identifier.issn0022-2615en_US
dc.identifier.urihttp://hdl.handle.net/10722/153957-
dc.description.abstractThe effect of Streptococcus sanguis, S. salivarius, Escherichia coli and Porphyromonas gingivalis on the adhesion of Candida albicans and C. krusei to human buccal epithelial cells (BEC) was investigated with a modified membrane filter system. The filters (12 μm diameter pores) acted as a support for the BEC which were pre-exposed to known concentrations of bacterial suspensions (for 45 min-1 h), and then re-incubated with standardised concentrations of yeast suspensions for various periods. The BEC with adherent yeasts were then transferred on to a glass slide, gram-stained and counted by light microscopy. Three of the four bacterial species significantly suppressed adhesion of C. albicans to BEC; S. sanguis had no effect. Both S. sanguis and S. salivarius suppressed adhesion of C. krusei to BEC pre-exposed to three different bacterial concentrations, although variable results were obtained with P. gingivalis and E. coli. Significant differences in the relative adhesion of C. albicans and C. krusei to BEC were also recorded. These results indicate that the adhesion of yeasts to BEC is modulated both by the composition and the quantity of the pre-existing bacterial flora on the BEC.en_US
dc.languageengen_US
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.orgen_US
dc.relation.ispartofJournal of Medical Microbiologyen_US
dc.subject.meshAdulten_US
dc.subject.meshBacterial Physiological Phenomenaen_US
dc.subject.meshCandida - Pathogenicity - Physiologyen_US
dc.subject.meshCell Adhesionen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshEpithelium - Microbiologyen_US
dc.subject.meshEscherichia Coli - Physiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMicroscopy, Electronen_US
dc.subject.meshMicroscopy, Electron, Scanningen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMouth Mucosa - Microbiology - Ultrastructureen_US
dc.subject.meshPorphyromonas Gingivalis - Physiologyen_US
dc.subject.meshStreptococcus - Physiologyen_US
dc.subject.meshStreptococcus Sanguis - Physiologyen_US
dc.titleThe effect of oral commensal bacteria on candidal adhesion to human buccal epithelial cells in vitroen_US
dc.typeArticleen_US
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1099/00222615-45-3-179-
dc.identifier.pmid8810944-
dc.identifier.scopuseid_2-s2.0-0029791708en_US
dc.identifier.hkuros20964-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029791708&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume45en_US
dc.identifier.issue3en_US
dc.identifier.spage179en_US
dc.identifier.epage185en_US
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridNair, RG=7202192241en_US
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_US

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