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Article: Newer saliva collection methods and saliva composition: a study of two Salivette kits.
Title | Newer saliva collection methods and saliva composition: a study of two Salivette kits. |
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Authors | |
Keywords | electrolytes glycoproteins proteins saliva Salivette |
Issue Date | 1995 |
Publisher | Wiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1354-523X&site=1 |
Citation | Oral Diseases, 1995, v. 1 n. 2, p. 86-91 How to Cite? |
Abstract | Saliva is frequently used as a diagnostic fluid and several collection devices have been developed. OBJECTIVE: The aim of the present study was to investigate the validity and reliability of two types of Salivette collection kits (non-covered cotton roll and polypropylene covered polyether roll) relative to conventional collection of saliva using paraffin wax chewing stimulation. MATERIALS AND METHODS: Whole saliva samples were collected from 16 healthy volunteers. Following a cross-over design saliva was collected in a standardized way. The flow rate was determined and saliva samples were analyzed for pH, buffer capacity, electrolytes and protein/glycoprotein content. RESULTS: We find that Salivette methods do not allow evaluation of flow rate. pH was unaffected but buffer capacity was lower in Salivette collected than in paraffin wax-stimulated saliva. The non-covered cotton rolls reduced the content of Na+, K+, Cl-, as well as glycoprotein markers (hexosamines, fucose, sialic acid), lysozyme, lactoferrin, salivary- and myeloperoxidase but increased the concentrations of Ca2+, PO4(3)- and SCN-. Polypropylene covered polyether rolls affected saliva composition less than the non-covered cotton rolls. Thus, SCN- and sIgA concentrations were higher and lysozyme activity lower in the former (covered roll) saliva than in paraffin wax saliva. The reliability of the Salivette kits was good. CONCLUSION: We conclude that the Salivette method generates data significantly different from conventional paraffin wax-stimulated saliva such as buffer capacity and several electrolytes and organic components. Care should be taken in interpreting the results when such methods are employed. |
Persistent Identifier | http://hdl.handle.net/10722/153932 |
ISSN | 2023 Impact Factor: 2.9 2023 SCImago Journal Rankings: 0.895 |
DC Field | Value | Language |
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dc.contributor.author | LenanderLumikari, M | en_US |
dc.contributor.author | Johansson, I | en_US |
dc.contributor.author | Vilja, P | en_US |
dc.contributor.author | Samaranayake, LP | en_US |
dc.date.accessioned | 2012-08-08T08:22:22Z | - |
dc.date.available | 2012-08-08T08:22:22Z | - |
dc.date.issued | 1995 | en_US |
dc.identifier.citation | Oral Diseases, 1995, v. 1 n. 2, p. 86-91 | en_US |
dc.identifier.issn | 1354-523X | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/153932 | - |
dc.description.abstract | Saliva is frequently used as a diagnostic fluid and several collection devices have been developed. OBJECTIVE: The aim of the present study was to investigate the validity and reliability of two types of Salivette collection kits (non-covered cotton roll and polypropylene covered polyether roll) relative to conventional collection of saliva using paraffin wax chewing stimulation. MATERIALS AND METHODS: Whole saliva samples were collected from 16 healthy volunteers. Following a cross-over design saliva was collected in a standardized way. The flow rate was determined and saliva samples were analyzed for pH, buffer capacity, electrolytes and protein/glycoprotein content. RESULTS: We find that Salivette methods do not allow evaluation of flow rate. pH was unaffected but buffer capacity was lower in Salivette collected than in paraffin wax-stimulated saliva. The non-covered cotton rolls reduced the content of Na+, K+, Cl-, as well as glycoprotein markers (hexosamines, fucose, sialic acid), lysozyme, lactoferrin, salivary- and myeloperoxidase but increased the concentrations of Ca2+, PO4(3)- and SCN-. Polypropylene covered polyether rolls affected saliva composition less than the non-covered cotton rolls. Thus, SCN- and sIgA concentrations were higher and lysozyme activity lower in the former (covered roll) saliva than in paraffin wax saliva. The reliability of the Salivette kits was good. CONCLUSION: We conclude that the Salivette method generates data significantly different from conventional paraffin wax-stimulated saliva such as buffer capacity and several electrolytes and organic components. Care should be taken in interpreting the results when such methods are employed. | en_US |
dc.language | eng | en_US |
dc.publisher | Wiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1354-523X&site=1 | en_US |
dc.relation.ispartof | Oral diseases | en_US |
dc.subject | electrolytes | - |
dc.subject | glycoproteins | - |
dc.subject | proteins | - |
dc.subject | saliva | - |
dc.subject | Salivette | - |
dc.subject.mesh | Adult | en_US |
dc.subject.mesh | Biological Markers | en_US |
dc.subject.mesh | Electrolytes - Analysis | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Glycoproteins - Analysis | en_US |
dc.subject.mesh | Gossypium | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Hydrogen-Ion Concentration | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Paraffin | en_US |
dc.subject.mesh | Polypropylenes | en_US |
dc.subject.mesh | Reproducibility Of Results | en_US |
dc.subject.mesh | Saliva - Chemistry - Secretion | en_US |
dc.subject.mesh | Salivary Proteins And Peptides - Analysis | en_US |
dc.subject.mesh | Specimen Handling - Instrumentation - Methods | en_US |
dc.title | Newer saliva collection methods and saliva composition: a study of two Salivette kits. | en_US |
dc.type | Article | en_US |
dc.identifier.email | Samaranayake, LP:lakshman@hku.hk | en_US |
dc.identifier.authority | Samaranayake, LP=rp00023 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 7553391 | - |
dc.identifier.scopus | eid_2-s2.0-0029316237 | en_US |
dc.identifier.hkuros | 9094 | - |
dc.identifier.hkuros | 3576 | - |
dc.identifier.volume | 1 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 86 | en_US |
dc.identifier.epage | 91 | en_US |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | LenanderLumikari, M=7003887944 | en_US |
dc.identifier.scopusauthorid | Johansson, I=7102079367 | en_US |
dc.identifier.scopusauthorid | Vilja, P=7003805228 | en_US |
dc.identifier.scopusauthorid | Samaranayake, LP=7102761002 | en_US |
dc.identifier.issnl | 1354-523X | - |