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Article: Anti-candida activity of murine bronchoalveolar lavage fluid

TitleAnti-candida activity of murine bronchoalveolar lavage fluid
Authors
Issue Date1994
PublisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org
Citation
Journal Of Medical Microbiology, 1994, v. 40 n. 5, p. 350-357 How to Cite?
AbstractRespiratory secretions provide an efficient method for protecting the large surface area of the lower respiratory tract. To determine whether lung secretions contribute to antifungal defences, murine bronchoalveolar lavage fluid (BLF) was tested for anti-candidal activity against 49 oral and vaginal isolates belonging to six different Candida species. The yeasts were incubated in unconcentrated, cell-free lavage fluid from Sprague-Dawley rats and then cultured quantitatively to measure residual viability. Experiments with C. albicans indicated that sensitivity to BLF increased in a time- and dose-dependent manner. This activity was heat-stable (56°C) and consistent, irrespective of whether the BLF was derived from rats inoculated (orally) with candida or the uninoculated controls. Of the Candida spp. examined, C. albicans was the most susceptible followed by C. parapsilosis and C. tropicalis, whereas C. krusei, C. guilliermondii and C. glabrata were highly resistant. However, there were differences in susceptibility to BLF among different isolates within a given species. These results indicate that a heat-stable, soluble factor(s) in murine lavage fluid may suppress candidal colonisation of the lower respiratory tract and contribute to the defence mechanisms of the lungs.
Persistent Identifierhttp://hdl.handle.net/10722/153860
ISSN
2015 Impact Factor: 2.269
2015 SCImago Journal Rankings: 1.060
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSamaranayake, LPen_US
dc.contributor.authorTobgi, RSen_US
dc.contributor.authorMacfarlane, TWen_US
dc.date.accessioned2012-08-08T08:21:57Z-
dc.date.available2012-08-08T08:21:57Z-
dc.date.issued1994en_US
dc.identifier.citationJournal Of Medical Microbiology, 1994, v. 40 n. 5, p. 350-357en_US
dc.identifier.issn0022-2615en_US
dc.identifier.urihttp://hdl.handle.net/10722/153860-
dc.description.abstractRespiratory secretions provide an efficient method for protecting the large surface area of the lower respiratory tract. To determine whether lung secretions contribute to antifungal defences, murine bronchoalveolar lavage fluid (BLF) was tested for anti-candidal activity against 49 oral and vaginal isolates belonging to six different Candida species. The yeasts were incubated in unconcentrated, cell-free lavage fluid from Sprague-Dawley rats and then cultured quantitatively to measure residual viability. Experiments with C. albicans indicated that sensitivity to BLF increased in a time- and dose-dependent manner. This activity was heat-stable (56°C) and consistent, irrespective of whether the BLF was derived from rats inoculated (orally) with candida or the uninoculated controls. Of the Candida spp. examined, C. albicans was the most susceptible followed by C. parapsilosis and C. tropicalis, whereas C. krusei, C. guilliermondii and C. glabrata were highly resistant. However, there were differences in susceptibility to BLF among different isolates within a given species. These results indicate that a heat-stable, soluble factor(s) in murine lavage fluid may suppress candidal colonisation of the lower respiratory tract and contribute to the defence mechanisms of the lungs.en_US
dc.languageengen_US
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.orgen_US
dc.relation.ispartofJournal of Medical Microbiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlood - Immunologyen_US
dc.subject.meshBronchoalveolar Lavage Fluid - Immunologyen_US
dc.subject.meshCandida - Immunologyen_US
dc.subject.meshCandidiasis, Oral - Microbiologyen_US
dc.subject.meshCandidiasis, Vulvovaginal - Microbiologyen_US
dc.subject.meshDose-Response Relationship, Immunologicen_US
dc.subject.meshFemaleen_US
dc.subject.meshHot Temperatureen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshTime Factorsen_US
dc.titleAnti-candida activity of murine bronchoalveolar lavage fluiden_US
dc.typeArticleen_US
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8176722-
dc.identifier.scopuseid_2-s2.0-0028227345en_US
dc.identifier.volume40en_US
dc.identifier.issue5en_US
dc.identifier.spage350en_US
dc.identifier.epage357en_US
dc.identifier.isiWOS:A1994NK96600008-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_US
dc.identifier.scopusauthoridTobgi, RS=36755102700en_US
dc.identifier.scopusauthoridMacFarlane, TW=7005128465en_US

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