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Article: Localized expression of mRNA for phagocyte-specific chemotactic cytokines in human periodontal infections

TitleLocalized expression of mRNA for phagocyte-specific chemotactic cytokines in human periodontal infections
Authors
Issue Date1994
Citation
Infection And Immunity, 1994, v. 62 n. 9, p. 4005-4014 How to Cite?
AbstractIn bacterial infections, mononuclear and polymorphonuclear phagocytes are key components of host defenses. Recent investigations have indicated that chemokines are able to recruit and activate phagocytes. In particular, interleukin-8 (IL-8) attracts polymorphonuclear leukocytes (PMNs), while monocyte chemoattractant protein-1 (MCP-1) is selective for cells of the monocyte/macrophage lineage. In this investigation, we analyzed the in situ expression of IL-8 and MCP-1 mRNAs in human periodontal infections. Specific mRNA was detected by in situ hybridization using 35S-labeled riboprobes in frozen tissue sections. Phagocytes (PMNs and macrophages) were specifically detected as elastase-positive or CD68+ cells by a three-stage immunoperoxidase technique. Results indicated that expression of phagocyte- specific cytokines was confined to selected tissue locations and, in general, paralleled phagocyte infiltration. In particular, IL-8 expression was maximal in the junctional epithelium adjacent to the infecting microorganisms; PMN infiltration was more prominent in the same area. MCP-1 was expressed in the chronic inflammatory infiltrate and along the basal layer of the oral epithelium. Cells of the monocyte/macrophage lineage were demonstrated to be present in the same areas. The observed expression pattern may be the most economic way to establish a cell-type-selective chemotactic gradient within the tissue that is able to effectively direct polymorphonuclear phagocyte migration toward the infecting microorganisms and modulate mononuclear phagocyte infiltration in the surrounding tissues. This process may optimize host defenses and contribute to containing leukocyte infiltration to the infected and inflamed area, thus limiting tissue damage.
Persistent Identifierhttp://hdl.handle.net/10722/153857
ISSN
2015 Impact Factor: 3.603
2015 SCImago Journal Rankings: 2.342
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTonetti, MSen_US
dc.contributor.authorImboden, MAen_US
dc.contributor.authorGerber, Len_US
dc.contributor.authorLang, NPen_US
dc.contributor.authorLaissue, Jen_US
dc.contributor.authorMueller, Cen_US
dc.date.accessioned2012-08-08T08:21:56Z-
dc.date.available2012-08-08T08:21:56Z-
dc.date.issued1994en_US
dc.identifier.citationInfection And Immunity, 1994, v. 62 n. 9, p. 4005-4014en_US
dc.identifier.issn0019-9567en_US
dc.identifier.urihttp://hdl.handle.net/10722/153857-
dc.description.abstractIn bacterial infections, mononuclear and polymorphonuclear phagocytes are key components of host defenses. Recent investigations have indicated that chemokines are able to recruit and activate phagocytes. In particular, interleukin-8 (IL-8) attracts polymorphonuclear leukocytes (PMNs), while monocyte chemoattractant protein-1 (MCP-1) is selective for cells of the monocyte/macrophage lineage. In this investigation, we analyzed the in situ expression of IL-8 and MCP-1 mRNAs in human periodontal infections. Specific mRNA was detected by in situ hybridization using 35S-labeled riboprobes in frozen tissue sections. Phagocytes (PMNs and macrophages) were specifically detected as elastase-positive or CD68+ cells by a three-stage immunoperoxidase technique. Results indicated that expression of phagocyte- specific cytokines was confined to selected tissue locations and, in general, paralleled phagocyte infiltration. In particular, IL-8 expression was maximal in the junctional epithelium adjacent to the infecting microorganisms; PMN infiltration was more prominent in the same area. MCP-1 was expressed in the chronic inflammatory infiltrate and along the basal layer of the oral epithelium. Cells of the monocyte/macrophage lineage were demonstrated to be present in the same areas. The observed expression pattern may be the most economic way to establish a cell-type-selective chemotactic gradient within the tissue that is able to effectively direct polymorphonuclear phagocyte migration toward the infecting microorganisms and modulate mononuclear phagocyte infiltration in the surrounding tissues. This process may optimize host defenses and contribute to containing leukocyte infiltration to the infected and inflamed area, thus limiting tissue damage.en_US
dc.languageengen_US
dc.relation.ispartofInfection and Immunityen_US
dc.subject.meshAntigens, Cd - Analysisen_US
dc.subject.meshAntigens, Differentiation, Myelomonocytic - Analysisen_US
dc.subject.meshChemokine Ccl2en_US
dc.subject.meshChemotactic Factors - Geneticsen_US
dc.subject.meshGingivitis - Metabolism - Pathologyen_US
dc.subject.meshHumansen_US
dc.subject.meshInterleukin-8 - Geneticsen_US
dc.subject.meshNeutrophils - Physiologyen_US
dc.subject.meshPeriodontitis - Metabolismen_US
dc.subject.meshPhagocytes - Physiologyen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.titleLocalized expression of mRNA for phagocyte-specific chemotactic cytokines in human periodontal infectionsen_US
dc.typeArticleen_US
dc.identifier.emailLang, NP:nplang@hkucc.hku.hken_US
dc.identifier.authorityLang, NP=rp00031en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8063420-
dc.identifier.scopuseid_2-s2.0-0028142785en_US
dc.identifier.volume62en_US
dc.identifier.issue9en_US
dc.identifier.spage4005en_US
dc.identifier.epage4014en_US
dc.identifier.isiWOS:A1994PC83600054-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridTonetti, MS=35602248900en_US
dc.identifier.scopusauthoridImboden, MA=9044173700en_US
dc.identifier.scopusauthoridGerber, L=7103016821en_US
dc.identifier.scopusauthoridLang, NP=7201577367en_US
dc.identifier.scopusauthoridLaissue, J=7007141334en_US
dc.identifier.scopusauthoridMueller, C=7202002170en_US

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