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Article: DNA probe detection of periodontopathogens in advanced periodontitis.

TitleDNA probe detection of periodontopathogens in advanced periodontitis.
Authors
Issue Date1993
Citation
Scandinavian Journal Of Dental Research, 1993, v. 101 n. 6, p. 363-370 How to Cite?
AbstractSpecies-specific DNA probes were used to determine the presence of Actinobacillus actinomycetemcomitans (A.a.), Porphyromonas (Bacteroides) gingivalis, Prevotella intermedia, Treponema denticola, Eikenella corrodens, Fusobacterium nucleatum, and Wolinella recta in subgingival plaque from deep pockets/sites of patients with advanced periodontitis. The subjects were 20 patients with severe adult periodontitis, 13 men and 7 women (mean age 45.6 +/- 6.7 yr). For each subject, 9-10 subgingival sites with the deepest probing depths from each quadrant were sampled by the paper point method, a total of 198 sites, with mean probing depth 7.2 +/- 1.6 mm and clinical attachment level 9.5 +/- 2.7 mm. A.a. was present in at least one site in 75% of the subjects; P. gingivalis was found in 95%; P. intermedia and W. recta were found in 90%, respectively; and T. denticola, E. corrodens, and F. nucleatum were found in all subjects. In the 198 samples, A.a. was detected in 25.8%, P. gingivalis in 51.5%, P. intermedia in 64.1%, T. denticola in 60.6%, E. corrodens in 72.9%, F. nucleatum in 74.7%, and W. recta in 65.7%. The predominant combination was the simultaneous presence of P. intermedia, T. denticola, E. corrodens, F. nucleatum, and W. recta in 89.5% of the subjects and 46.8% of the sites. Of these sites, 51.1% showed the combined presence of P. gingivalis and 28.4% that of both A.a. and P. gingivalis. None of the seven bacteria could be detected in 14.4% of the total sites sampled. The present study indicates that severe destructive adult periodontitis is a multibacterial infection and that certain combinations of periodontopathogens seem to be important in the pathogenesis of the disease.
Persistent Identifierhttp://hdl.handle.net/10722/153842
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSöder, POen_US
dc.contributor.authorJin, LJen_US
dc.contributor.authorSöder, Ben_US
dc.date.accessioned2012-08-08T08:21:52Z-
dc.date.available2012-08-08T08:21:52Z-
dc.date.issued1993en_US
dc.identifier.citationScandinavian Journal Of Dental Research, 1993, v. 101 n. 6, p. 363-370en_US
dc.identifier.issn0029-845Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/153842-
dc.description.abstractSpecies-specific DNA probes were used to determine the presence of Actinobacillus actinomycetemcomitans (A.a.), Porphyromonas (Bacteroides) gingivalis, Prevotella intermedia, Treponema denticola, Eikenella corrodens, Fusobacterium nucleatum, and Wolinella recta in subgingival plaque from deep pockets/sites of patients with advanced periodontitis. The subjects were 20 patients with severe adult periodontitis, 13 men and 7 women (mean age 45.6 +/- 6.7 yr). For each subject, 9-10 subgingival sites with the deepest probing depths from each quadrant were sampled by the paper point method, a total of 198 sites, with mean probing depth 7.2 +/- 1.6 mm and clinical attachment level 9.5 +/- 2.7 mm. A.a. was present in at least one site in 75% of the subjects; P. gingivalis was found in 95%; P. intermedia and W. recta were found in 90%, respectively; and T. denticola, E. corrodens, and F. nucleatum were found in all subjects. In the 198 samples, A.a. was detected in 25.8%, P. gingivalis in 51.5%, P. intermedia in 64.1%, T. denticola in 60.6%, E. corrodens in 72.9%, F. nucleatum in 74.7%, and W. recta in 65.7%. The predominant combination was the simultaneous presence of P. intermedia, T. denticola, E. corrodens, F. nucleatum, and W. recta in 89.5% of the subjects and 46.8% of the sites. Of these sites, 51.1% showed the combined presence of P. gingivalis and 28.4% that of both A.a. and P. gingivalis. None of the seven bacteria could be detected in 14.4% of the total sites sampled. The present study indicates that severe destructive adult periodontitis is a multibacterial infection and that certain combinations of periodontopathogens seem to be important in the pathogenesis of the disease.en_US
dc.languageengen_US
dc.relation.ispartofScandinavian Journal of Dental Researchen_US
dc.subject.meshActinobacillus Actinomycetemcomitans - Genetics - Isolation & Purificationen_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshBacterial Typing Techniquesen_US
dc.subject.meshBacteroides - Genetics - Isolation & Purificationen_US
dc.subject.meshColony Count, Microbialen_US
dc.subject.meshDna Probes - Diagnostic Useen_US
dc.subject.meshDna, Bacterial - Analysisen_US
dc.subject.meshDental Plaque - Microbiologyen_US
dc.subject.meshEcologyen_US
dc.subject.meshEikenella Corrodens - Genetics - Isolation & Purificationen_US
dc.subject.meshFemaleen_US
dc.subject.meshFusobacterium Nucleatum - Genetics - Isolation & Purificationen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPeriodontal Pocket - Microbiologyen_US
dc.subject.meshPeriodontitis - Microbiologyen_US
dc.subject.meshPorphyromonas Gingivalis - Genetics - Isolation & Purificationen_US
dc.subject.meshTreponema - Genetics - Isolation & Purificationen_US
dc.subject.meshVirulenceen_US
dc.subject.meshWolinella - Genetics - Isolation & Purificationen_US
dc.titleDNA probe detection of periodontopathogens in advanced periodontitis.en_US
dc.typeArticleen_US
dc.identifier.emailJin, LJ:ljjin@hkucc.hku.hken_US
dc.identifier.authorityJin, LJ=rp00028en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8290878-
dc.identifier.scopuseid_2-s2.0-0027737824en_US
dc.identifier.volume101en_US
dc.identifier.issue6en_US
dc.identifier.spage363en_US
dc.identifier.epage370en_US
dc.identifier.isiWOS:A1993MK47700004-
dc.identifier.scopusauthoridSöder, PO=7006570984en_US
dc.identifier.scopusauthoridJin, LJ=7403328850en_US
dc.identifier.scopusauthoridSöder, B=6701795462en_US

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