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Article: Reproducibility of microscopic and cultural data in repeated subgingival plaque samples.

TitleReproducibility of microscopic and cultural data in repeated subgingival plaque samples.
Authors
Issue Date1989
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE
Citation
Journal Of Clinical Periodontology, 1989, v. 16 n. 7, p. 434-442 How to Cite?
AbstractIn association studies, micro-organisms can only be recognized as suspects for playing a major rôle in the development of a pathological environment, if their destructive action goes along with a marked proportional increase of their numbers or if their first detection can be related to the clinical onset of the disease. Limitations in the reproducibility of repeated samples have to be taken into account, when changes of the microbial composition of subgingival environments are to be studied, and when local clinical changes are to be related to shifts in the composition of the pertaining microbiological compartment. To study reproducibility, a total of 109 sites was sampled repeatedly with sterile paperpoints at an interval of 7 to 10 days in 24 patients suffering from periodontal disease and 12 edentulous patients wearing successful and failing osseointegrated titanium implants. Using continuous anaerobic techniques, the samples were cultured on nonselective and selective media and were studied by darkfield microscopy. Both the intertest-agreements of frequencies of detection (kappa-statistics) as well as the discrepancies of proportions of bacterial groups and selected bacterial species were determined. The standard deviation of proportional differences between first and second samples ranged between 6.4% (fusiform organisms) and 17.2% (coccoid cells) for darkfield parameters, between 4.3% (B. melaninogenicus on ETSA/Kana.) and 14.0% (B. gingivalis on ETSA/Kana.) for selected bacterial species and between 6.9% (gram-negative anaerobic cocci) and 24.0% (gram-positive facultative cocci) for bacterial groups classified according to gram stain characteristics and atmospheric growth conditions.(ABSTRACT TRUNCATED AT 250 WORDS)
Persistent Identifierhttp://hdl.handle.net/10722/153684
ISSN
2015 Impact Factor: 3.915
2015 SCImago Journal Rankings: 1.848
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMombelli, Aen_US
dc.contributor.authorMinder, CEen_US
dc.contributor.authorGusberti, FAen_US
dc.contributor.authorLang, NPen_US
dc.date.accessioned2012-08-08T08:21:02Z-
dc.date.available2012-08-08T08:21:02Z-
dc.date.issued1989en_US
dc.identifier.citationJournal Of Clinical Periodontology, 1989, v. 16 n. 7, p. 434-442en_US
dc.identifier.issn0303-6979en_US
dc.identifier.urihttp://hdl.handle.net/10722/153684-
dc.description.abstractIn association studies, micro-organisms can only be recognized as suspects for playing a major rôle in the development of a pathological environment, if their destructive action goes along with a marked proportional increase of their numbers or if their first detection can be related to the clinical onset of the disease. Limitations in the reproducibility of repeated samples have to be taken into account, when changes of the microbial composition of subgingival environments are to be studied, and when local clinical changes are to be related to shifts in the composition of the pertaining microbiological compartment. To study reproducibility, a total of 109 sites was sampled repeatedly with sterile paperpoints at an interval of 7 to 10 days in 24 patients suffering from periodontal disease and 12 edentulous patients wearing successful and failing osseointegrated titanium implants. Using continuous anaerobic techniques, the samples were cultured on nonselective and selective media and were studied by darkfield microscopy. Both the intertest-agreements of frequencies of detection (kappa-statistics) as well as the discrepancies of proportions of bacterial groups and selected bacterial species were determined. The standard deviation of proportional differences between first and second samples ranged between 6.4% (fusiform organisms) and 17.2% (coccoid cells) for darkfield parameters, between 4.3% (B. melaninogenicus on ETSA/Kana.) and 14.0% (B. gingivalis on ETSA/Kana.) for selected bacterial species and between 6.9% (gram-negative anaerobic cocci) and 24.0% (gram-positive facultative cocci) for bacterial groups classified according to gram stain characteristics and atmospheric growth conditions.(ABSTRACT TRUNCATED AT 250 WORDS)en_US
dc.languageengen_US
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPEen_US
dc.relation.ispartofJournal of Clinical Periodontologyen_US
dc.subject.meshBacteria - Classification - Isolation & Purificationen_US
dc.subject.meshBacteroides - Isolation & Purificationen_US
dc.subject.meshDental Implantation, Endosseousen_US
dc.subject.meshDental Plaque - Microbiologyen_US
dc.subject.meshGingivaen_US
dc.subject.meshGram-Negative Bacteria - Isolation & Purificationen_US
dc.subject.meshGram-Positive Bacteria - Isolation & Purificationen_US
dc.subject.meshHumansen_US
dc.subject.meshJaw, Edentulous - Microbiologyen_US
dc.subject.meshMicroscopy - Methodsen_US
dc.subject.meshPeriodontal Diseases - Microbiologyen_US
dc.subject.meshSpirochaetales - Isolation & Purificationen_US
dc.subject.meshTime Factorsen_US
dc.titleReproducibility of microscopic and cultural data in repeated subgingival plaque samples.en_US
dc.typeArticleen_US
dc.identifier.emailLang, NP:nplang@hkucc.hku.hken_US
dc.identifier.authorityLang, NP=rp00031en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid2768538-
dc.identifier.scopuseid_2-s2.0-0024710959en_US
dc.identifier.volume16en_US
dc.identifier.issue7en_US
dc.identifier.spage434en_US
dc.identifier.epage442en_US
dc.identifier.isiWOS:A1989AP23800007-
dc.publisher.placeDenmarken_US
dc.identifier.scopusauthoridMombelli, A=7006180872en_US
dc.identifier.scopusauthoridMinder, CE=35425884100en_US
dc.identifier.scopusauthoridGusberti, FA=6604050465en_US
dc.identifier.scopusauthoridLang, NP=7201577367en_US

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