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Article: Factors affecting the in vitro adherence of the fungal oral pathogen Candida albicans to epithelial cells of human origin
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TitleFactors affecting the in vitro adherence of the fungal oral pathogen Candida albicans to epithelial cells of human origin
 
AuthorsSamaranayake, LP1
Macfarlane, TW1
 
Issue Date1982
 
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio
 
CitationArchives Of Oral Biology, 1982, v. 27 n. 10, p. 869-873 [How to Cite?]
 
AbstractAn in-vitro technique was used to investigate the effects of oral commensal bacteria, serum, saliva, germ-tube formation and pH on the adherence of two strains of C. albicans to HeLa cells. Streptococcus salivarius and Streptococcus mitior reduced candidal adhesion whereas Streptococcus mutans had no significant effect. A mixed salivary pellicle on HeLa cells significantly enhanced candidal adhesion and a serum layer had no effect. Yeasts pre-incubated in whole saliva for 3 h showed significantly greater adhesion to HeLa and human embryonic kidney epithelial cells than yeasts in phosphate-buffered saline. The adherence of hyphal phase candida was significantly greater than in the blastospore phase. Adhesion varied with the pH of the test medium, maximal adherence was at pH 3 with less under neutral pH conditions. The factors involved in the adhesion of C. albicans to epithelial surfaces are complex and may play a role in the aetiopathology of human mucosal candidoses.
 
ISSN0003-9969
2013 Impact Factor: 1.880
2013 SCImago Journal Rankings: 0.677
 
ISI Accession Number IDWOS:A1982PM61100013
 
DC FieldValue
dc.contributor.authorSamaranayake, LP
 
dc.contributor.authorMacfarlane, TW
 
dc.date.accessioned2012-08-08T08:20:19Z
 
dc.date.available2012-08-08T08:20:19Z
 
dc.date.issued1982
 
dc.description.abstractAn in-vitro technique was used to investigate the effects of oral commensal bacteria, serum, saliva, germ-tube formation and pH on the adherence of two strains of C. albicans to HeLa cells. Streptococcus salivarius and Streptococcus mitior reduced candidal adhesion whereas Streptococcus mutans had no significant effect. A mixed salivary pellicle on HeLa cells significantly enhanced candidal adhesion and a serum layer had no effect. Yeasts pre-incubated in whole saliva for 3 h showed significantly greater adhesion to HeLa and human embryonic kidney epithelial cells than yeasts in phosphate-buffered saline. The adherence of hyphal phase candida was significantly greater than in the blastospore phase. Adhesion varied with the pH of the test medium, maximal adherence was at pH 3 with less under neutral pH conditions. The factors involved in the adhesion of C. albicans to epithelial surfaces are complex and may play a role in the aetiopathology of human mucosal candidoses.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationArchives Of Oral Biology, 1982, v. 27 n. 10, p. 869-873 [How to Cite?]
 
dc.identifier.epage873
 
dc.identifier.isiWOS:A1982PM61100013
 
dc.identifier.issn0003-9969
2013 Impact Factor: 1.880
2013 SCImago Journal Rankings: 0.677
 
dc.identifier.issue10
 
dc.identifier.pmid6760838
 
dc.identifier.scopuseid_2-s2.0-0020402345
 
dc.identifier.spage869
 
dc.identifier.urihttp://hdl.handle.net/10722/153549
 
dc.identifier.volume27
 
dc.languageeng
 
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofArchives of Oral Biology
 
dc.subject.meshAdhesiveness
 
dc.subject.meshCandida Albicans - Physiology
 
dc.subject.meshCells, Cultured
 
dc.subject.meshDental Pellicle
 
dc.subject.meshEpithelium
 
dc.subject.meshHela Cells
 
dc.subject.meshHumans
 
dc.subject.meshHydrogen-Ion Concentration
 
dc.subject.meshSaliva
 
dc.subject.meshStreptococcus - Physiology
 
dc.subject.meshStreptococcus Mutans - Physiology
 
dc.titleFactors affecting the in vitro adherence of the fungal oral pathogen Candida albicans to epithelial cells of human origin
 
dc.typeArticle
 
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<item><contributor.author>Samaranayake, LP</contributor.author>
<contributor.author>Macfarlane, TW</contributor.author>
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<date.available>2012-08-08T08:20:19Z</date.available>
<date.issued>1982</date.issued>
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<description.abstract>An in-vitro technique was used to investigate the effects of oral commensal bacteria, serum, saliva, germ-tube formation and pH on the adherence of two strains of C. albicans to HeLa cells. Streptococcus salivarius and Streptococcus mitior reduced candidal adhesion whereas Streptococcus mutans had no significant effect. A mixed salivary pellicle on HeLa cells significantly enhanced candidal adhesion and a serum layer had no effect. Yeasts pre-incubated in whole saliva for 3 h showed significantly greater adhesion to HeLa and human embryonic kidney epithelial cells than yeasts in phosphate-buffered saline. The adherence of hyphal phase candida was significantly greater than in the blastospore phase. Adhesion varied with the pH of the test medium, maximal adherence was at pH 3 with less under neutral pH conditions. The factors involved in the adhesion of C. albicans to epithelial surfaces are complex and may play a role in the aetiopathology of human mucosal candidoses.</description.abstract>
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<subject.mesh>Adhesiveness</subject.mesh>
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<subject.mesh>Cells, Cultured</subject.mesh>
<subject.mesh>Dental Pellicle</subject.mesh>
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<subject.mesh>Humans</subject.mesh>
<subject.mesh>Hydrogen-Ion Concentration</subject.mesh>
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Author Affiliations
  1. Glasgow Dental Hospital and School