Article: Factors affecting the in vitro adherence of the fungal oral pathogen Candida albicans to epithelial cells of human origin

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TitleFactors affecting the in vitro adherence of the fungal oral pathogen Candida albicans to epithelial cells of human origin
AuthorsSamaranayake, LP1
Macfarlane, TW1
Issue Date1982
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio
CitationArchives Of Oral Biology, 1982, v. 27 n. 10, p. 869-873 [How to Cite?]
AbstractAn in-vitro technique was used to investigate the effects of oral commensal bacteria, serum, saliva, germ-tube formation and pH on the adherence of two strains of C. albicans to HeLa cells. Streptococcus salivarius and Streptococcus mitior reduced candidal adhesion whereas Streptococcus mutans had no significant effect. A mixed salivary pellicle on HeLa cells significantly enhanced candidal adhesion and a serum layer had no effect. Yeasts pre-incubated in whole saliva for 3 h showed significantly greater adhesion to HeLa and human embryonic kidney epithelial cells than yeasts in phosphate-buffered saline. The adherence of hyphal phase candida was significantly greater than in the blastospore phase. Adhesion varied with the pH of the test medium, maximal adherence was at pH 3 with less under neutral pH conditions. The factors involved in the adhesion of C. albicans to epithelial surfaces are complex and may play a role in the aetiopathology of human mucosal candidoses.
ISSN0003-9969
2011 Impact Factor: 1.603
2011 SCImago Journal Rankings: 0.095
ISI Accession Number IDWOS:A1982PM61100013
DC Field
Value
dc.contributor.authorSamaranayake, LP
dc.contributor.authorMacfarlane, TW
dc.date.accessioned2012-08-08T08:20:19Z
dc.date.available2012-08-08T08:20:19Z
dc.date.issued1982
dc.description.abstractAn in-vitro technique was used to investigate the effects of oral commensal bacteria, serum, saliva, germ-tube formation and pH on the adherence of two strains of C. albicans to HeLa cells. Streptococcus salivarius and Streptococcus mitior reduced candidal adhesion whereas Streptococcus mutans had no significant effect. A mixed salivary pellicle on HeLa cells significantly enhanced candidal adhesion and a serum layer had no effect. Yeasts pre-incubated in whole saliva for 3 h showed significantly greater adhesion to HeLa and human embryonic kidney epithelial cells than yeasts in phosphate-buffered saline. The adherence of hyphal phase candida was significantly greater than in the blastospore phase. Adhesion varied with the pH of the test medium, maximal adherence was at pH 3 with less under neutral pH conditions. The factors involved in the adhesion of C. albicans to epithelial surfaces are complex and may play a role in the aetiopathology of human mucosal candidoses.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationArchives Of Oral Biology, 1982, v. 27 n. 10, p. 869-873 [How to Cite?]
dc.identifier.epage873
dc.identifier.isiWOS:A1982PM61100013
dc.identifier.issn0003-9969
2011 Impact Factor: 1.603
2011 SCImago Journal Rankings: 0.095
dc.identifier.issue10
dc.identifier.pmid6760838
dc.identifier.scopuseid_2-s2.0-0020402345
dc.identifier.spage869
dc.identifier.urihttp://hdl.handle.net/10722/153549
dc.identifier.volume27
dc.languageeng
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio
dc.publisher.placeUnited Kingdom
dc.relation.ispartofArchives of Oral Biology
dc.subject.meshAdhesiveness
dc.subject.meshCandida Albicans - Physiology
dc.subject.meshCells, Cultured
dc.subject.meshDental Pellicle
dc.subject.meshEpithelium
dc.subject.meshHela Cells
dc.subject.meshHumans
dc.subject.meshHydrogen-Ion Concentration
dc.subject.meshSaliva
dc.subject.meshStreptococcus - Physiology
dc.subject.meshStreptococcus Mutans - Physiology
dc.titleFactors affecting the in vitro adherence of the fungal oral pathogen Candida albicans to epithelial cells of human origin
dc.typeArticle
Author Affiliations
  1. Glasgow Dental Hospital and School