File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Cell mediated immune responses to plaque antigens during experimental gingivitis in man

TitleCell mediated immune responses to plaque antigens during experimental gingivitis in man
Authors
Issue Date1978
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1
Citation
Journal Of Periodontal Research, 1978, v. 13 n. 3, p. 232-239 How to Cite?
AbstractThe purpose of the present investigation was to evaluate the ability of antigens of human dental plaque microorganisms to stimulate peripheral blood lymphocytes (PBL) from humans during the development and recovery from experimental gingivitis. Eleven healthy dental students (22-36 years) participated in the study. At the start of the experiment, they exhibited clean teeth and clinically healthy gingivae. They then abstained from all oral hygiene measures for four weeks according to the model proposed by Loe et al. (1965). At the start, after 1, 2, 3 and 4 weeks of abolished oral hygiene and 2 weeks following reinstitution of oral hygiene, the oral conditions were assessed, using the criteria of the Plaque Index system (Loe & Silness 1964) and the Gingival Index system (Loe & Silness 1963). At each examination triplicate microcultures, each containing 2 x 105 viable PBL in .2 ml TC 199 with glutamine and antibiotics containing 10% fetal calf serum, were stimulated with four concentrations of sonicates from V. alcalescens, F. nucleatum, B. melaninogenicus, A. viscosus, A. naeslundii, S. sanguis and pooled dental plaque all obtained from a previously performed experimental gingivitis. The cultures were incubated for 78 hours at 37°C in 5% CO2. 3H-thymidine was added for the final 8 hours. Only A. viscosus was able to stimulate PBL to undergo blastogenesis during the development of experimental gingivitis. A. viscosus stimulated the PBL of 3 subjects after one week but all subjects after 2 weeks of abolished oral hygiene. The stimulation indices (SI) remained > 2.5 for the rest of the experimental period. Following 2 weeks of reinstituted oral hygiene, the SI reached preexperimental levels. These results suggest that A. viscosus is capable of inducing a specific cellular immune response during the development of experimental gingivitis and may be important as a causative plaque constituent. Further research is indicated to study this association.
Persistent Identifierhttp://hdl.handle.net/10722/153519
ISSN
2015 Impact Factor: 2.474
2015 SCImago Journal Rankings: 0.932
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSmith, FNen_US
dc.contributor.authorLang, NPen_US
dc.contributor.authorLoe, HAen_US
dc.date.accessioned2012-08-08T08:20:09Z-
dc.date.available2012-08-08T08:20:09Z-
dc.date.issued1978en_US
dc.identifier.citationJournal Of Periodontal Research, 1978, v. 13 n. 3, p. 232-239en_US
dc.identifier.issn0022-3484en_US
dc.identifier.urihttp://hdl.handle.net/10722/153519-
dc.description.abstractThe purpose of the present investigation was to evaluate the ability of antigens of human dental plaque microorganisms to stimulate peripheral blood lymphocytes (PBL) from humans during the development and recovery from experimental gingivitis. Eleven healthy dental students (22-36 years) participated in the study. At the start of the experiment, they exhibited clean teeth and clinically healthy gingivae. They then abstained from all oral hygiene measures for four weeks according to the model proposed by Loe et al. (1965). At the start, after 1, 2, 3 and 4 weeks of abolished oral hygiene and 2 weeks following reinstitution of oral hygiene, the oral conditions were assessed, using the criteria of the Plaque Index system (Loe & Silness 1964) and the Gingival Index system (Loe & Silness 1963). At each examination triplicate microcultures, each containing 2 x 105 viable PBL in .2 ml TC 199 with glutamine and antibiotics containing 10% fetal calf serum, were stimulated with four concentrations of sonicates from V. alcalescens, F. nucleatum, B. melaninogenicus, A. viscosus, A. naeslundii, S. sanguis and pooled dental plaque all obtained from a previously performed experimental gingivitis. The cultures were incubated for 78 hours at 37°C in 5% CO2. 3H-thymidine was added for the final 8 hours. Only A. viscosus was able to stimulate PBL to undergo blastogenesis during the development of experimental gingivitis. A. viscosus stimulated the PBL of 3 subjects after one week but all subjects after 2 weeks of abolished oral hygiene. The stimulation indices (SI) remained > 2.5 for the rest of the experimental period. Following 2 weeks of reinstituted oral hygiene, the SI reached preexperimental levels. These results suggest that A. viscosus is capable of inducing a specific cellular immune response during the development of experimental gingivitis and may be important as a causative plaque constituent. Further research is indicated to study this association.en_US
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1en_US
dc.relation.ispartofJournal of Periodontal Researchen_US
dc.subject.meshAdulten_US
dc.subject.meshAntigens, Bacterialen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshDental Plaque - Immunologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshGingivitis - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunity, Cellularen_US
dc.subject.meshLymphocyte Activationen_US
dc.subject.meshLymphocytes - Immunologyen_US
dc.subject.meshMaleen_US
dc.titleCell mediated immune responses to plaque antigens during experimental gingivitis in manen_US
dc.typeArticleen_US
dc.identifier.emailLang, NP:nplang@hkucc.hku.hken_US
dc.identifier.authorityLang, NP=rp00031en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid149193-
dc.identifier.scopuseid_2-s2.0-0017809656en_US
dc.identifier.volume13en_US
dc.identifier.issue3en_US
dc.identifier.spage232en_US
dc.identifier.epage239en_US
dc.identifier.isiWOS:A1978EZ31000007-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSmith, FN=7402856529en_US
dc.identifier.scopusauthoridLang, NP=7201577367en_US
dc.identifier.scopusauthoridLoe, HA=7103064570en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats