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Article: Lymphocyte response to T cell mitogen during experimental gingivitis in humans

TitleLymphocyte response to T cell mitogen during experimental gingivitis in humans
Authors
Issue Date1976
Citation
Infection And Immunity, 1976, v. 13 n. 1, p. 108-113 How to Cite?
AbstractThis study was conducted to evaluate the dose response relationships of peripheral blood lymphocytes (PBL) by stimulation of phytohemagglutinin (PHA) during the onset of oral inflammation. Eleven dental students underwent a 3 week experimental gingivitis program. At time zero, weeks 1, 2, and 3, and after 1 week of reinstituted oral hygiene (week 4), the plaque accumulations were evaluated, the degree of gingival inflammation was assessed, and a blood sample was taken. Quadruplicate microcultures each containing 2 x 105 PBL in 0.2 ml of tissue culture medium 199 and 10% fetal calf serum were stimulated with five concentrations of PHA (10 to 0.5 μg/ml) and incubated for 78 h at 37 C in 5% CO2. [3H]thymidine was added to each culture for the final 8 h. The cultures were then harvested and counted by liquid scintillation, and stimulation indexes (SI) were determined. At time zero the maximum PBL response occurred at a PHA concentration of 5 μg/ml (SI = 100). During weeks 1, 2, and 3 the location of the maximum PBL response shifted to a lower PHA concentration (1.0 μg/ml) and increased to over SI = 400. The phenomenon of shifting of peak PHA responses to lower PHA concentrations could be observed after only 1 week of developing gingival inflammation. The PBL response returned to pre experimental values after 1 week of reinstituted oral hygiene, which resolved the oral inflammation. The findings show that a dose response relationship exists between PHA concentrations and the PBL response. If these dose response changes seen during developing gingival inflammation are ignored, either a decrease, increase, or no change in PBL response can be shown depending upon the PHA concentration evaluated. Owing to the dose dependent nature of this PBL response, it is advisable to routinely use dose response curves in order to properly evaluate the full responsiveness of PBL to mitogenic substances.
Persistent Identifierhttp://hdl.handle.net/10722/153512
ISSN
2015 Impact Factor: 3.603
2015 SCImago Journal Rankings: 2.342
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLang, NPen_US
dc.contributor.authorSmith, FNen_US
dc.date.accessioned2012-08-08T08:20:07Z-
dc.date.available2012-08-08T08:20:07Z-
dc.date.issued1976en_US
dc.identifier.citationInfection And Immunity, 1976, v. 13 n. 1, p. 108-113en_US
dc.identifier.issn0019-9567en_US
dc.identifier.urihttp://hdl.handle.net/10722/153512-
dc.description.abstractThis study was conducted to evaluate the dose response relationships of peripheral blood lymphocytes (PBL) by stimulation of phytohemagglutinin (PHA) during the onset of oral inflammation. Eleven dental students underwent a 3 week experimental gingivitis program. At time zero, weeks 1, 2, and 3, and after 1 week of reinstituted oral hygiene (week 4), the plaque accumulations were evaluated, the degree of gingival inflammation was assessed, and a blood sample was taken. Quadruplicate microcultures each containing 2 x 105 PBL in 0.2 ml of tissue culture medium 199 and 10% fetal calf serum were stimulated with five concentrations of PHA (10 to 0.5 μg/ml) and incubated for 78 h at 37 C in 5% CO2. [3H]thymidine was added to each culture for the final 8 h. The cultures were then harvested and counted by liquid scintillation, and stimulation indexes (SI) were determined. At time zero the maximum PBL response occurred at a PHA concentration of 5 μg/ml (SI = 100). During weeks 1, 2, and 3 the location of the maximum PBL response shifted to a lower PHA concentration (1.0 μg/ml) and increased to over SI = 400. The phenomenon of shifting of peak PHA responses to lower PHA concentrations could be observed after only 1 week of developing gingival inflammation. The PBL response returned to pre experimental values after 1 week of reinstituted oral hygiene, which resolved the oral inflammation. The findings show that a dose response relationship exists between PHA concentrations and the PBL response. If these dose response changes seen during developing gingival inflammation are ignored, either a decrease, increase, or no change in PBL response can be shown depending upon the PHA concentration evaluated. Owing to the dose dependent nature of this PBL response, it is advisable to routinely use dose response curves in order to properly evaluate the full responsiveness of PBL to mitogenic substances.en_US
dc.languageengen_US
dc.relation.ispartofInfection and Immunityen_US
dc.subject.meshAdulten_US
dc.subject.meshDental Plaque - Diagnosisen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshGingivitis - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshLymphocyte Activation - Drug Effectsen_US
dc.subject.meshMitogens - Pharmacologyen_US
dc.subject.meshT-Lymphocytesen_US
dc.titleLymphocyte response to T cell mitogen during experimental gingivitis in humansen_US
dc.typeArticleen_US
dc.identifier.emailLang, NP:nplang@hkucc.hku.hken_US
dc.identifier.authorityLang, NP=rp00031en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1082442-
dc.identifier.scopuseid_2-s2.0-0017294096en_US
dc.identifier.volume13en_US
dc.identifier.issue1en_US
dc.identifier.spage108en_US
dc.identifier.epage113en_US
dc.identifier.isiWOS:A1976BC77900016-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLang, NP=7201577367en_US
dc.identifier.scopusauthoridSmith, FN=7402856529en_US

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