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Article: Analysis of complement-bound HCV complexes using a novel immuno-capture RT-PCR method

TitleAnalysis of complement-bound HCV complexes using a novel immuno-capture RT-PCR method
Authors
Issue Date2002
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/SJI
Citation
Scandinavian Journal of Immunology, 2002, v. 56 n. 5, p. 538-542 How to Cite?
AbstractRecently, more and more evidence has supported the hypothesis that liver cell injury was immune-mediated in patients with hepatitis C virus (HCV) infection, and that circulating immune complexes (CICs) might play a role in the pathogenesis of chronic hepatitis C (HC). In the present study, we have combined immuno-capture and reverse transcriptase-polymerase chain reaction (RT-PCR), and developed a quick method of high specificity for the detection of complement-bound HCV-CIC. We found that there were higher frequencies of HCV-C1q CIC than that of HCV-factor B, and there was a deviation of complement from immunoglobulin (Ig) in HCV-CIC. These findings suggest that immuno-capture RT-PCR (iRT-PCR) for the detection of HCV-bound CIC is a valuable method for the analysis of the composition of the immune complexes, and for the understanding of host immune response and immune pathogenesis in HCV-infected individuals.
Persistent Identifierhttp://hdl.handle.net/10722/153492
ISSN
2015 Impact Factor: 2.27
2015 SCImago Journal Rankings: 0.934
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhu, H-
dc.contributor.authorWang, SY-
dc.contributor.authorZhang, JY-
dc.contributor.authorLian, Q-
dc.contributor.authorPeng, XX-
dc.date.accessioned2012-08-07T06:59:48Z-
dc.date.available2012-08-07T06:59:48Z-
dc.date.issued2002-
dc.identifier.citationScandinavian Journal of Immunology, 2002, v. 56 n. 5, p. 538-542-
dc.identifier.issn0300-9475-
dc.identifier.urihttp://hdl.handle.net/10722/153492-
dc.description.abstractRecently, more and more evidence has supported the hypothesis that liver cell injury was immune-mediated in patients with hepatitis C virus (HCV) infection, and that circulating immune complexes (CICs) might play a role in the pathogenesis of chronic hepatitis C (HC). In the present study, we have combined immuno-capture and reverse transcriptase-polymerase chain reaction (RT-PCR), and developed a quick method of high specificity for the detection of complement-bound HCV-CIC. We found that there were higher frequencies of HCV-C1q CIC than that of HCV-factor B, and there was a deviation of complement from immunoglobulin (Ig) in HCV-CIC. These findings suggest that immuno-capture RT-PCR (iRT-PCR) for the detection of HCV-bound CIC is a valuable method for the analysis of the composition of the immune complexes, and for the understanding of host immune response and immune pathogenesis in HCV-infected individuals.-
dc.languageeng-
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/SJI-
dc.relation.ispartofScandinavian Journal of Immunology-
dc.rightsThe definitive version is available at www.blackwell-synergy.com-
dc.subject.meshComplement C1q - analysis - metabolism-
dc.subject.meshHepacivirus - immunology - isolation and purification-
dc.subject.meshHepatitis C, Chronic - immunology - virology-
dc.subject.meshRNA, Viral - blood - genetics-
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction - statistics and numerical data-
dc.titleAnalysis of complement-bound HCV complexes using a novel immuno-capture RT-PCR methoden_US
dc.typeArticleen_US
dc.identifier.emailLian, Q: dalilian2000@yahoo.com.cn-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1365-3083.2002.01164.x-
dc.identifier.pmid12410805-
dc.identifier.scopuseid_2-s2.0-0036434085-
dc.identifier.volume56-
dc.identifier.issue5-
dc.identifier.spage538-
dc.identifier.epage542-
dc.identifier.isiWOS:000178966600015-
dc.publisher.placeUnited Kingdom-

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