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Article: Analysis of complement-bound hepatitis B Virus complexes by an immuno-capture polymerase chain reaction method

TitleAnalysis of complement-bound hepatitis B Virus complexes by an immuno-capture polymerase chain reaction method
Authors
Issue Date2003
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/SJI
Citation
Scandinavian Journal of Immunology, 2003, v. 58 n. 1, p. 112-116 How to Cite?
AbstractHepatitis B Virus (HBV) can be present in the circulating blood either as a free virus or as a virion-immunoglobulin (Ig) complex with or without complement. However, information regarding the complement-bound HBV circulating immune complexes (CIC) in HBV-infected patients is currently not available. In the present study, we have combined immuno-capture and polymerase chain reaction (PCR) and developed a quick method of high specificity for the detection of complement-bound HBV CIC. We found that the frequency of HBV-factor B was associated with clinical types of hepatitis B (HB) but not with that of HBV-C1q. Moreover, increased frequency at P < 0.05 were found for HBV/C1q-CIC in the group with normal total bilirubin (TBIL) and for HBV/factor B-CIC in the group with positive hepatitis B e antigen (HBeAg). These findings suggest that the immuno-capture PCR (iPCR) for the detection of HBV-bound CIC is a valuable method for analysis of the composition of the immune complexes and for the understanding of host immune response and immune pathogenesis in HBV-infected individuals. In summary, iPCR is a valuable method for analysis of the composition of the immune complexes, which may provide new and valuable insights into HBV pathogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/153491
ISSN
2015 Impact Factor: 2.27
2015 SCImago Journal Rankings: 0.934
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWang, SY-
dc.contributor.authorLiu, R-
dc.contributor.authorZhang, JY-
dc.contributor.authorLian, Q-
dc.contributor.authorPeng, XX-
dc.date.accessioned2012-08-07T06:52:24Z-
dc.date.available2012-08-07T06:52:24Z-
dc.date.issued2003-
dc.identifier.citationScandinavian Journal of Immunology, 2003, v. 58 n. 1, p. 112-116-
dc.identifier.issn0300-9475-
dc.identifier.urihttp://hdl.handle.net/10722/153491-
dc.description.abstractHepatitis B Virus (HBV) can be present in the circulating blood either as a free virus or as a virion-immunoglobulin (Ig) complex with or without complement. However, information regarding the complement-bound HBV circulating immune complexes (CIC) in HBV-infected patients is currently not available. In the present study, we have combined immuno-capture and polymerase chain reaction (PCR) and developed a quick method of high specificity for the detection of complement-bound HBV CIC. We found that the frequency of HBV-factor B was associated with clinical types of hepatitis B (HB) but not with that of HBV-C1q. Moreover, increased frequency at P < 0.05 were found for HBV/C1q-CIC in the group with normal total bilirubin (TBIL) and for HBV/factor B-CIC in the group with positive hepatitis B e antigen (HBeAg). These findings suggest that the immuno-capture PCR (iPCR) for the detection of HBV-bound CIC is a valuable method for analysis of the composition of the immune complexes and for the understanding of host immune response and immune pathogenesis in HBV-infected individuals. In summary, iPCR is a valuable method for analysis of the composition of the immune complexes, which may provide new and valuable insights into HBV pathogenesis.-
dc.languageeng-
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/SJI-
dc.relation.ispartofScandinavian Journal of Immunology-
dc.rightsThe definitive version is available at www.blackwell-synergy.com-
dc.subject.meshAntigen-Antibody Complex - analysis-
dc.subject.meshComplement System Proteins - metabolism-
dc.subject.meshHepatitis B virus - immunology-
dc.subject.meshPolymerase Chain Reaction - methods-
dc.titleAnalysis of complement-bound hepatitis B Virus complexes by an immuno-capture polymerase chain reaction methoden_US
dc.typeArticleen_US
dc.identifier.emailLian, Q: dalilian2000@yahoo.com.cn-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1046/j.1365-3083.2003.01282.x-
dc.identifier.pmid12828566-
dc.identifier.scopuseid_2-s2.0-0038346598-
dc.identifier.volume58-
dc.identifier.issue1-
dc.identifier.spage112-
dc.identifier.epage116-
dc.identifier.isiWOS:000184006700015-
dc.publisher.placeUnited Kingdom-

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