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Conference Paper: A model for directed embryonic stem cell differentiation: Establishing lineage-restricted progenitor cell lines

TitleA model for directed embryonic stem cell differentiation: Establishing lineage-restricted progenitor cell lines
Authors
Issue Date2005
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/modo
Citation
The 15th International Society of Developmental Biologists Congress, Sydney, Australia, 3-7 September 2005. In Mechanisms of Development, 2005, v. 122 n. S1, p. S133, Abstract no. 09-P023 How to Cite?
AbstractThe promise of ESC as a panacea for many diseases is based on an unproven expectation that the pluripotency and highly proliferative capacity of ESC lines can be directed to generate sufficient therapeutic cell types. A possible solution is to restrict the differentiation potential of ESC lines to specific cell types without losing their proliferative capacity by establishing lineagerestricted cell lines from ESCs. Here we describe the establishment of clonal, ESC-derived non-pluripotent progenitor cell lines with endothelial potential. Differentiating ESCs from disaggregated embryoid bodies (EBs) were propagated as a monolayer to screen for rare presumptive colonies of progenitor cells with large nucleus to cytoplasm ratio for establishing clonal cell lines. Some of these clonal lines, E-RoSH, resembled the previously described RoSH2 mouse embryonic endothelial progenitor cell lines. Unlike the parental ES cells, E-RoSH cells do not express pluripotent genes, cannot form teratomas in SCID mice and do not express nestin when cultured in suspension. They express high levels of mesodermal genes and differentiate readily to form endothelial cells when plated on matrigel or transplanted into a teratoma.
Persistent Identifierhttp://hdl.handle.net/10722/153490
ISSN
2015 Impact Factor: 2.041
2015 SCImago Journal Rankings: 1.368

 

DC FieldValueLanguage
dc.contributor.authorLian, Q-
dc.contributor.authorLim, SK-
dc.contributor.authorQue, J-
dc.contributor.authorJames, B-
dc.contributor.authorSalto-Tellez, M-
dc.contributor.authorYin, Y-
dc.contributor.authorTan, E-
dc.contributor.authorYeo, K-
dc.contributor.authorYu, F-
dc.contributor.authorOakley, R-
dc.date.accessioned2012-08-07T06:37:46Z-
dc.date.available2012-08-07T06:37:46Z-
dc.date.issued2005-
dc.identifier.citationThe 15th International Society of Developmental Biologists Congress, Sydney, Australia, 3-7 September 2005. In Mechanisms of Development, 2005, v. 122 n. S1, p. S133, Abstract no. 09-P023-
dc.identifier.issn0925-4773-
dc.identifier.urihttp://hdl.handle.net/10722/153490-
dc.description.abstractThe promise of ESC as a panacea for many diseases is based on an unproven expectation that the pluripotency and highly proliferative capacity of ESC lines can be directed to generate sufficient therapeutic cell types. A possible solution is to restrict the differentiation potential of ESC lines to specific cell types without losing their proliferative capacity by establishing lineagerestricted cell lines from ESCs. Here we describe the establishment of clonal, ESC-derived non-pluripotent progenitor cell lines with endothelial potential. Differentiating ESCs from disaggregated embryoid bodies (EBs) were propagated as a monolayer to screen for rare presumptive colonies of progenitor cells with large nucleus to cytoplasm ratio for establishing clonal cell lines. Some of these clonal lines, E-RoSH, resembled the previously described RoSH2 mouse embryonic endothelial progenitor cell lines. Unlike the parental ES cells, E-RoSH cells do not express pluripotent genes, cannot form teratomas in SCID mice and do not express nestin when cultured in suspension. They express high levels of mesodermal genes and differentiate readily to form endothelial cells when plated on matrigel or transplanted into a teratoma.-
dc.languageeng-
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/modo-
dc.relation.ispartofMechanisms of Development-
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Mechanisms of Development. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Mechanisms of Development, [VOL 122, ISSUE S1, 2005] DOI 10.1016/j.mod.2005.06.010-
dc.titleA model for directed embryonic stem cell differentiation: Establishing lineage-restricted progenitor cell linesen_US
dc.typeConference_Paperen_US
dc.identifier.emailLian, Q: dalilian2000@yahoo.com.cn-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.mod.2005.06.010-
dc.identifier.volume122-
dc.identifier.issueS1-
dc.identifier.spageS133-
dc.identifier.epageS133-
dc.publisher.placeIreland-
dc.description.otherThe 15th International Society of Developmental Biologists Congress, Sydney, Australia, 3-7 September 2005. In Mechanisms of Development, 2005, v. 122 n. S1, p. S133, Abstract no. 09-P023-

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