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Article: Naringin attenuates EGF-induced MUC5AC secretion in A549 cells by suppressing the cooperative activities of MAPKs-AP-1 and IKKs-IκB-NF- κB signaling pathways

TitleNaringin attenuates EGF-induced MUC5AC secretion in A549 cells by suppressing the cooperative activities of MAPKs-AP-1 and IKKs-IκB-NF- κB signaling pathways
Authors
KeywordsActivator protein-1
Epithelium growth factor
Epithelium growth factor receptor
MUC5AC
Naringin
Nuclear factor kappa B
Issue Date2012
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejphar
Citation
European Journal Of Pharmacology, 2012, v. 690 n. 1-3, p. 207-213 How to Cite?
AbstractNaringenin, the aglycone of naringin, has been reported to attenuate MUC5AC secretion by inhibiting activity of nuclear factor kappa B (NF-κB) via EGFR-PI3K-Akt/ERK MAPKinase signaling pathways. However, previous studies demonstrated that the MUC5AC promoter was located in two different regions: an activator protein-1 (AP-1) binding site and a NF-κB binding site. The current study comprehensively determined the involvement of MAPKs/AP-1 and IKKs/IκB/NF-κB in epidermal growth factor (EGF)-induced A549 cells, and sought to ascertain the signaling pathways of naringin imparted in suppression of EGF-induced MUC5AC secretion. The results showed that naringin of 100 μM not only significantly decreased EGF-induced overexpressions of both MUC5AC mucin and mRNA in A549 cells, but also suppressed the phosphorylation of EGF receptor, p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase (ERK1/2), and c-Jun N-terminal kinase (JNK), as well as nucleus NF-κB p65 and AP-1. Moreover, any of three MAPKs inhibitors (PD98059, SB203580, and SP600125) significantly inhibited EGF-induced MUC5AC secretion. And as compared to MG132, the inhibitor κB (IκB) phosphorylation inhibitor of SN50 was more effective in reducing EGF-induced MUC5AC secretion because of suppression of nucleus AP-1. Meanwhile, as compared to naringin, both SP600125 and azithromycin were less effective in suppressing EGF-induced secretion of MUC5AC because of the unchanged nucleus NF-κB p65. These results indicated that naringin attenuates EGF-induced MUC5AC secretion in A549 cells by suppressing the cooperative activities of MAPKs/AP-1 and IKKs/IκB/NF-κB signaling pathways. © 2012 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/153239
ISSN
2015 Impact Factor: 2.73
2015 SCImago Journal Rankings: 1.115
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNie, YCen_HK
dc.contributor.authorWu, Hen_HK
dc.contributor.authorLi, PBen_HK
dc.contributor.authorXie, LMen_HK
dc.contributor.authorLuo, YLen_HK
dc.contributor.authorShen, JGen_HK
dc.contributor.authorSu, WWen_HK
dc.date.accessioned2012-07-16T10:01:18Z-
dc.date.available2012-07-16T10:01:18Z-
dc.date.issued2012en_HK
dc.identifier.citationEuropean Journal Of Pharmacology, 2012, v. 690 n. 1-3, p. 207-213en_HK
dc.identifier.issn0014-2999en_HK
dc.identifier.urihttp://hdl.handle.net/10722/153239-
dc.description.abstractNaringenin, the aglycone of naringin, has been reported to attenuate MUC5AC secretion by inhibiting activity of nuclear factor kappa B (NF-κB) via EGFR-PI3K-Akt/ERK MAPKinase signaling pathways. However, previous studies demonstrated that the MUC5AC promoter was located in two different regions: an activator protein-1 (AP-1) binding site and a NF-κB binding site. The current study comprehensively determined the involvement of MAPKs/AP-1 and IKKs/IκB/NF-κB in epidermal growth factor (EGF)-induced A549 cells, and sought to ascertain the signaling pathways of naringin imparted in suppression of EGF-induced MUC5AC secretion. The results showed that naringin of 100 μM not only significantly decreased EGF-induced overexpressions of both MUC5AC mucin and mRNA in A549 cells, but also suppressed the phosphorylation of EGF receptor, p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase (ERK1/2), and c-Jun N-terminal kinase (JNK), as well as nucleus NF-κB p65 and AP-1. Moreover, any of three MAPKs inhibitors (PD98059, SB203580, and SP600125) significantly inhibited EGF-induced MUC5AC secretion. And as compared to MG132, the inhibitor κB (IκB) phosphorylation inhibitor of SN50 was more effective in reducing EGF-induced MUC5AC secretion because of suppression of nucleus AP-1. Meanwhile, as compared to naringin, both SP600125 and azithromycin were less effective in suppressing EGF-induced secretion of MUC5AC because of the unchanged nucleus NF-κB p65. These results indicated that naringin attenuates EGF-induced MUC5AC secretion in A549 cells by suppressing the cooperative activities of MAPKs/AP-1 and IKKs/IκB/NF-κB signaling pathways. © 2012 Elsevier B.V.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejpharen_HK
dc.relation.ispartofEuropean Journal of Pharmacologyen_HK
dc.subjectActivator protein-1en_HK
dc.subjectEpithelium growth factoren_HK
dc.subjectEpithelium growth factor receptoren_HK
dc.subjectMUC5ACen_HK
dc.subjectNaringinen_HK
dc.subjectNuclear factor kappa Ben_HK
dc.titleNaringin attenuates EGF-induced MUC5AC secretion in A549 cells by suppressing the cooperative activities of MAPKs-AP-1 and IKKs-IκB-NF- κB signaling pathwaysen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-2999&volume=&spage=&epage=&date=2012&atitle=Naringin+attenuates+EGF-induced+MUC5AC+secretion+in+A549+cells+by+suppressing+the+cooperative+activities+of+MAPKs-AP-1+and+IKKs-IκB-NF-κB+signaling+pathways.en_US
dc.identifier.emailShen, JG: shenjg@hku.hken_HK
dc.identifier.authorityShen, JG=rp00487en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ejphar.2012.06.040en_HK
dc.identifier.pmid22766066-
dc.identifier.scopuseid_2-s2.0-84864423315en_HK
dc.identifier.hkuros201615en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84864423315&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume690en_HK
dc.identifier.issue1-3en_HK
dc.identifier.spage207en_HK
dc.identifier.epage213en_HK
dc.identifier.isiWOS:000306965200028-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridNie, YC=53865296000en_HK
dc.identifier.scopusauthoridWu, H=35222773000en_HK
dc.identifier.scopusauthoridLi, PB=10141281200en_HK
dc.identifier.scopusauthoridXie, LM=55324969700en_HK
dc.identifier.scopusauthoridLuo, YL=37026466900en_HK
dc.identifier.scopusauthoridShen, JG=7404929947en_HK
dc.identifier.scopusauthoridSu, WW=7402010268en_HK
dc.identifier.citeulike10850790-

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