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Article: Identification of the receptors for prolactin-releasing peptide (PrRP) and Carassius RFamide peptide (C-RFa) in chickens

TitleIdentification of the receptors for prolactin-releasing peptide (PrRP) and Carassius RFamide peptide (C-RFa) in chickens
Authors
Issue Date2012
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 2012, v. 153 n. 4, p. 1861-1874 How to Cite?
AbstractProlactin-releasing peptide (PrRP) and its structurally related peptide, Carassius Arg-Phe-amide peptide (C-RFa), have been reported to play similar roles in regulating food intake and pituitary functions in vertebrates. However, the identity, functionality, and expression of the receptor(s) for PrRP and C-RFa remain largely unknown in nonmammalian vertebrates, including birds. In this study, three receptors homologous to mammalian PrRP receptor (PrRPR), named cPrRPR1, cPrRPR2, and cC-RFaR, respectively, were cloned from chicken brain by RT-PCR. Using a pGL3-NFAT-RE-luciferase reporter system, we demonstrated that cPrRPR1 and cPrRPR2 expressed in Chinese hamster ovarian cells could be activated by cPrRP 20 and cC-RFa 20 potently, whereas cC-RFaR could only be activated effectively by cC-RFa 20 (EC 50, 0.11 nM), indicating that cPrRPR1 and cPrRPR2 can function as common receptors for PrRP and C-RFa, whereas cC-RFaR is a receptor specific to C-RFa. Using a pGL3-CRE-luciferase reporter system, cPrRPR1, cPrRPR2, and cC-RFaR expressed in Chinese hamster ovarian cells were also shown to activate intracellular protein kinase A signaling pathway upon cC-RFa 20 treatment (100 nM). Moreover, RT-PCR assay revealed that cPrRPR1, cPrRPR2, and cC-RFaR were widely expressed in most adult chicken tissues examined, including various regions of brain. These findings, together with evidence of PrRP and C-RFa encoded by separate genes in chicken, Xenopus, and zebrafish, and the differential expression of PrRP and C-RFa genes in chicken tissues, strongly suggest that PrRP and C-RFa may play similar yet distinctive roles in nonmammalian vertebrates, including chicken, and their actions are mediated by common receptor(s) or a specific C-RFa receptor. Copyright © 2012 by The Endocrine Society.
Persistent Identifierhttp://hdl.handle.net/10722/153211
ISSN
2014 Impact Factor: 4.503
2013 SCImago Journal Rankings: 2.478
ISI Accession Number ID
Funding AgencyGrant Number
National Natural Science Foundation of China30871338
30971569
30771171
Funding Information:

This work was supported by National Natural Science Foundation of China Grants 30871338, 30971569, and 30771171.

References

 

DC FieldValueLanguage
dc.contributor.authorWang, Yen_HK
dc.contributor.authorWang, CYen_HK
dc.contributor.authorWu, Yen_HK
dc.contributor.authorHuang, Gen_HK
dc.contributor.authorLi, Jen_HK
dc.contributor.authorLeung, FCen_HK
dc.date.accessioned2012-07-16T09:59:51Z-
dc.date.available2012-07-16T09:59:51Z-
dc.date.issued2012en_HK
dc.identifier.citationEndocrinology, 2012, v. 153 n. 4, p. 1861-1874en_HK
dc.identifier.issn0013-7227en_HK
dc.identifier.urihttp://hdl.handle.net/10722/153211-
dc.description.abstractProlactin-releasing peptide (PrRP) and its structurally related peptide, Carassius Arg-Phe-amide peptide (C-RFa), have been reported to play similar roles in regulating food intake and pituitary functions in vertebrates. However, the identity, functionality, and expression of the receptor(s) for PrRP and C-RFa remain largely unknown in nonmammalian vertebrates, including birds. In this study, three receptors homologous to mammalian PrRP receptor (PrRPR), named cPrRPR1, cPrRPR2, and cC-RFaR, respectively, were cloned from chicken brain by RT-PCR. Using a pGL3-NFAT-RE-luciferase reporter system, we demonstrated that cPrRPR1 and cPrRPR2 expressed in Chinese hamster ovarian cells could be activated by cPrRP 20 and cC-RFa 20 potently, whereas cC-RFaR could only be activated effectively by cC-RFa 20 (EC 50, 0.11 nM), indicating that cPrRPR1 and cPrRPR2 can function as common receptors for PrRP and C-RFa, whereas cC-RFaR is a receptor specific to C-RFa. Using a pGL3-CRE-luciferase reporter system, cPrRPR1, cPrRPR2, and cC-RFaR expressed in Chinese hamster ovarian cells were also shown to activate intracellular protein kinase A signaling pathway upon cC-RFa 20 treatment (100 nM). Moreover, RT-PCR assay revealed that cPrRPR1, cPrRPR2, and cC-RFaR were widely expressed in most adult chicken tissues examined, including various regions of brain. These findings, together with evidence of PrRP and C-RFa encoded by separate genes in chicken, Xenopus, and zebrafish, and the differential expression of PrRP and C-RFa genes in chicken tissues, strongly suggest that PrRP and C-RFa may play similar yet distinctive roles in nonmammalian vertebrates, including chicken, and their actions are mediated by common receptor(s) or a specific C-RFa receptor. Copyright © 2012 by The Endocrine Society.en_HK
dc.languageengen_US
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_HK
dc.relation.ispartofEndocrinologyen_HK
dc.subject.meshAmino Acid Sequence-
dc.subject.meshBrain - metabolism-
dc.subject.meshChickens - metabolism-
dc.subject.meshNeuropeptides - genetics - metabolism-
dc.subject.meshReceptors, Neuropeptide - genetics - metabolism-
dc.titleIdentification of the receptors for prolactin-releasing peptide (PrRP) and Carassius RFamide peptide (C-RFa) in chickensen_HK
dc.typeArticleen_HK
dc.identifier.emailWang, Y: cdwyj@yahoo.comen_HK
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_HK
dc.identifier.authorityWang, Y=rp00801en_HK
dc.identifier.authorityLeung, FC=rp00731en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1210/en.2011-1719en_HK
dc.identifier.pmid22355069-
dc.identifier.scopuseid_2-s2.0-84859413087en_HK
dc.identifier.hkuros200943en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84859413087&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume153en_HK
dc.identifier.issue4en_HK
dc.identifier.spage1861en_HK
dc.identifier.epage1874en_HK
dc.identifier.isiWOS:000302169800034-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWang, Y=36062525200en_HK
dc.identifier.scopusauthoridWang, CY=16065173000en_HK
dc.identifier.scopusauthoridWu, Y=55175207900en_HK
dc.identifier.scopusauthoridHuang, G=55069829200en_HK
dc.identifier.scopusauthoridLi, J=27171035300en_HK
dc.identifier.scopusauthoridLeung, FC=7103078633en_HK

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