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Article: Identification of the receptors for prolactin-releasing peptide (PrRP) and Carassius RFamide peptide (C-RFa) in chickens
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TitleIdentification of the receptors for prolactin-releasing peptide (PrRP) and Carassius RFamide peptide (C-RFa) in chickens
 
AuthorsWang, Y2
Wang, CY1
Wu, Y2
Huang, G2
Li, J2
Leung, FC1
 
Issue Date2012
 
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
 
CitationEndocrinology, 2012, v. 153 n. 4, p. 1861-1874 [How to Cite?]
DOI: http://dx.doi.org/10.1210/en.2011-1719
 
AbstractProlactin-releasing peptide (PrRP) and its structurally related peptide, Carassius Arg-Phe-amide peptide (C-RFa), have been reported to play similar roles in regulating food intake and pituitary functions in vertebrates. However, the identity, functionality, and expression of the receptor(s) for PrRP and C-RFa remain largely unknown in nonmammalian vertebrates, including birds. In this study, three receptors homologous to mammalian PrRP receptor (PrRPR), named cPrRPR1, cPrRPR2, and cC-RFaR, respectively, were cloned from chicken brain by RT-PCR. Using a pGL3-NFAT-RE-luciferase reporter system, we demonstrated that cPrRPR1 and cPrRPR2 expressed in Chinese hamster ovarian cells could be activated by cPrRP 20 and cC-RFa 20 potently, whereas cC-RFaR could only be activated effectively by cC-RFa 20 (EC 50, 0.11 nM), indicating that cPrRPR1 and cPrRPR2 can function as common receptors for PrRP and C-RFa, whereas cC-RFaR is a receptor specific to C-RFa. Using a pGL3-CRE-luciferase reporter system, cPrRPR1, cPrRPR2, and cC-RFaR expressed in Chinese hamster ovarian cells were also shown to activate intracellular protein kinase A signaling pathway upon cC-RFa 20 treatment (100 nM). Moreover, RT-PCR assay revealed that cPrRPR1, cPrRPR2, and cC-RFaR were widely expressed in most adult chicken tissues examined, including various regions of brain. These findings, together with evidence of PrRP and C-RFa encoded by separate genes in chicken, Xenopus, and zebrafish, and the differential expression of PrRP and C-RFa genes in chicken tissues, strongly suggest that PrRP and C-RFa may play similar yet distinctive roles in nonmammalian vertebrates, including chicken, and their actions are mediated by common receptor(s) or a specific C-RFa receptor. Copyright © 2012 by The Endocrine Society.
 
ISSN0013-7227
2012 Impact Factor: 4.717
2012 SCImago Journal Rankings: 1.805
 
DOIhttp://dx.doi.org/10.1210/en.2011-1719
 
ISI Accession Number IDWOS:000302169800034
Funding AgencyGrant Number
National Natural Science Foundation of China30871338
30971569
30771171
Funding Information:

This work was supported by National Natural Science Foundation of China Grants 30871338, 30971569, and 30771171.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorWang, Y
 
dc.contributor.authorWang, CY
 
dc.contributor.authorWu, Y
 
dc.contributor.authorHuang, G
 
dc.contributor.authorLi, J
 
dc.contributor.authorLeung, FC
 
dc.date.accessioned2012-07-16T09:59:51Z
 
dc.date.available2012-07-16T09:59:51Z
 
dc.date.issued2012
 
dc.description.abstractProlactin-releasing peptide (PrRP) and its structurally related peptide, Carassius Arg-Phe-amide peptide (C-RFa), have been reported to play similar roles in regulating food intake and pituitary functions in vertebrates. However, the identity, functionality, and expression of the receptor(s) for PrRP and C-RFa remain largely unknown in nonmammalian vertebrates, including birds. In this study, three receptors homologous to mammalian PrRP receptor (PrRPR), named cPrRPR1, cPrRPR2, and cC-RFaR, respectively, were cloned from chicken brain by RT-PCR. Using a pGL3-NFAT-RE-luciferase reporter system, we demonstrated that cPrRPR1 and cPrRPR2 expressed in Chinese hamster ovarian cells could be activated by cPrRP 20 and cC-RFa 20 potently, whereas cC-RFaR could only be activated effectively by cC-RFa 20 (EC 50, 0.11 nM), indicating that cPrRPR1 and cPrRPR2 can function as common receptors for PrRP and C-RFa, whereas cC-RFaR is a receptor specific to C-RFa. Using a pGL3-CRE-luciferase reporter system, cPrRPR1, cPrRPR2, and cC-RFaR expressed in Chinese hamster ovarian cells were also shown to activate intracellular protein kinase A signaling pathway upon cC-RFa 20 treatment (100 nM). Moreover, RT-PCR assay revealed that cPrRPR1, cPrRPR2, and cC-RFaR were widely expressed in most adult chicken tissues examined, including various regions of brain. These findings, together with evidence of PrRP and C-RFa encoded by separate genes in chicken, Xenopus, and zebrafish, and the differential expression of PrRP and C-RFa genes in chicken tissues, strongly suggest that PrRP and C-RFa may play similar yet distinctive roles in nonmammalian vertebrates, including chicken, and their actions are mediated by common receptor(s) or a specific C-RFa receptor. Copyright © 2012 by The Endocrine Society.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationEndocrinology, 2012, v. 153 n. 4, p. 1861-1874 [How to Cite?]
DOI: http://dx.doi.org/10.1210/en.2011-1719
 
dc.identifier.doihttp://dx.doi.org/10.1210/en.2011-1719
 
dc.identifier.epage1874
 
dc.identifier.hkuros200943
 
dc.identifier.isiWOS:000302169800034
Funding AgencyGrant Number
National Natural Science Foundation of China30871338
30971569
30771171
Funding Information:

This work was supported by National Natural Science Foundation of China Grants 30871338, 30971569, and 30771171.

 
dc.identifier.issn0013-7227
2012 Impact Factor: 4.717
2012 SCImago Journal Rankings: 1.805
 
dc.identifier.issue4
 
dc.identifier.pmid22355069
 
dc.identifier.scopuseid_2-s2.0-84859413087
 
dc.identifier.spage1861
 
dc.identifier.urihttp://hdl.handle.net/10722/153211
 
dc.identifier.volume153
 
dc.languageeng
 
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
 
dc.publisher.placeUnited States
 
dc.relation.ispartofEndocrinology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAmino Acid Sequence
 
dc.subject.meshBrain - metabolism
 
dc.subject.meshChickens - metabolism
 
dc.subject.meshNeuropeptides - genetics - metabolism
 
dc.subject.meshReceptors, Neuropeptide - genetics - metabolism
 
dc.titleIdentification of the receptors for prolactin-releasing peptide (PrRP) and Carassius RFamide peptide (C-RFa) in chickens
 
dc.typeArticle
 
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<contributor.author>Li, J</contributor.author>
<contributor.author>Leung, FC</contributor.author>
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Author Affiliations
  1. The University of Hong Kong
  2. Sichuan University