Article: Identification of the receptors for prolactin-releasing peptide (PrRP) and Carassius RFamide peptide (C-RFa) in chickens
| Title | Identification of the receptors for prolactin-releasing peptide (PrRP) and Carassius RFamide peptide (C-RFa) in chickens | ||||
|---|---|---|---|---|---|
| Authors | Wang, Y2 Wang, CY1 Wu, Y2 Huang, G2 Li, J2 Leung, FC1 | ||||
| Issue Date | 2012 | ||||
| Publisher | The Endocrine Society. The Journal's web site is located at http://endo.endojournals.org | ||||
| Citation | Endocrinology, 2012, v. 153 n. 4, p. 1861-1874 [How to Cite?] DOI: http://dx.doi.org/10.1210/en.2011-1719 | ||||
| Abstract | Prolactin-releasing peptide (PrRP) and its structurally related peptide, Carassius Arg-Phe-amide peptide (C-RFa), have been reported to play similar roles in regulating food intake and pituitary functions in vertebrates. However, the identity, functionality, and expression of the receptor(s) for PrRP and C-RFa remain largely unknown in nonmammalian vertebrates, including birds. In this study, three receptors homologous to mammalian PrRP receptor (PrRPR), named cPrRPR1, cPrRPR2, and cC-RFaR, respectively, were cloned from chicken brain by RT-PCR. Using a pGL3-NFAT-RE-luciferase reporter system, we demonstrated that cPrRPR1 and cPrRPR2 expressed in Chinese hamster ovarian cells could be activated by cPrRP 20 and cC-RFa 20 potently, whereas cC-RFaR could only be activated effectively by cC-RFa 20 (EC 50, 0.11 nM), indicating that cPrRPR1 and cPrRPR2 can function as common receptors for PrRP and C-RFa, whereas cC-RFaR is a receptor specific to C-RFa. Using a pGL3-CRE-luciferase reporter system, cPrRPR1, cPrRPR2, and cC-RFaR expressed in Chinese hamster ovarian cells were also shown to activate intracellular protein kinase A signaling pathway upon cC-RFa 20 treatment (100 nM). Moreover, RT-PCR assay revealed that cPrRPR1, cPrRPR2, and cC-RFaR were widely expressed in most adult chicken tissues examined, including various regions of brain. These findings, together with evidence of PrRP and C-RFa encoded by separate genes in chicken, Xenopus, and zebrafish, and the differential expression of PrRP and C-RFa genes in chicken tissues, strongly suggest that PrRP and C-RFa may play similar yet distinctive roles in nonmammalian vertebrates, including chicken, and their actions are mediated by common receptor(s) or a specific C-RFa receptor. Copyright © 2012 by The Endocrine Society. | ||||
| ISSN | 0013-7227 2011 Impact Factor: 4.459 2011 SCImago Journal Rankings: 0.447 | ||||
| DOI | http://dx.doi.org/10.1210/en.2011-1719 | ||||
| ISI Accession Number ID | WOS:000302169800034
Funding Information: This work was supported by National Natural Science Foundation of China Grants 30871338, 30971569, and 30771171. | ||||
| References | References in Scopus |
| dc.contributor.author | Wang, Y | ||||
|---|---|---|---|---|---|
| dc.contributor.author | Wang, CY | ||||
| dc.contributor.author | Wu, Y | ||||
| dc.contributor.author | Huang, G | ||||
| dc.contributor.author | Li, J | ||||
| dc.contributor.author | Leung, FC | ||||
| dc.date.accessioned | 2012-07-16T09:59:51Z | ||||
| dc.date.available | 2012-07-16T09:59:51Z | ||||
| dc.date.issued | 2012 | ||||
| dc.description.abstract | Prolactin-releasing peptide (PrRP) and its structurally related peptide, Carassius Arg-Phe-amide peptide (C-RFa), have been reported to play similar roles in regulating food intake and pituitary functions in vertebrates. However, the identity, functionality, and expression of the receptor(s) for PrRP and C-RFa remain largely unknown in nonmammalian vertebrates, including birds. In this study, three receptors homologous to mammalian PrRP receptor (PrRPR), named cPrRPR1, cPrRPR2, and cC-RFaR, respectively, were cloned from chicken brain by RT-PCR. Using a pGL3-NFAT-RE-luciferase reporter system, we demonstrated that cPrRPR1 and cPrRPR2 expressed in Chinese hamster ovarian cells could be activated by cPrRP 20 and cC-RFa 20 potently, whereas cC-RFaR could only be activated effectively by cC-RFa 20 (EC 50, 0.11 nM), indicating that cPrRPR1 and cPrRPR2 can function as common receptors for PrRP and C-RFa, whereas cC-RFaR is a receptor specific to C-RFa. Using a pGL3-CRE-luciferase reporter system, cPrRPR1, cPrRPR2, and cC-RFaR expressed in Chinese hamster ovarian cells were also shown to activate intracellular protein kinase A signaling pathway upon cC-RFa 20 treatment (100 nM). Moreover, RT-PCR assay revealed that cPrRPR1, cPrRPR2, and cC-RFaR were widely expressed in most adult chicken tissues examined, including various regions of brain. These findings, together with evidence of PrRP and C-RFa encoded by separate genes in chicken, Xenopus, and zebrafish, and the differential expression of PrRP and C-RFa genes in chicken tissues, strongly suggest that PrRP and C-RFa may play similar yet distinctive roles in nonmammalian vertebrates, including chicken, and their actions are mediated by common receptor(s) or a specific C-RFa receptor. Copyright © 2012 by The Endocrine Society. | ||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||
| dc.identifier.citation | Endocrinology, 2012, v. 153 n. 4, p. 1861-1874 [How to Cite?] DOI: http://dx.doi.org/10.1210/en.2011-1719 | ||||
| dc.identifier.doi | http://dx.doi.org/10.1210/en.2011-1719 | ||||
| dc.identifier.epage | 1874 | ||||
| dc.identifier.hkuros | 200943 | ||||
| dc.identifier.isi | WOS:000302169800034
Funding Information: This work was supported by National Natural Science Foundation of China Grants 30871338, 30971569, and 30771171. | ||||
| dc.identifier.issn | 0013-7227 2011 Impact Factor: 4.459 2011 SCImago Journal Rankings: 0.447 | ||||
| dc.identifier.issue | 4 | ||||
| dc.identifier.pmid | 22355069 | ||||
| dc.identifier.scopus | eid_2-s2.0-84859413087 | ||||
| dc.identifier.spage | 1861 | ||||
| dc.identifier.uri | http://hdl.handle.net/10722/153211 | ||||
| dc.identifier.volume | 153 | ||||
| dc.language | eng | ||||
| dc.publisher | The Endocrine Society. The Journal's web site is located at http://endo.endojournals.org | ||||
| dc.publisher.place | United States | ||||
| dc.relation.ispartof | Endocrinology | ||||
| dc.relation.references | References in Scopus | ||||
| dc.subject.mesh | Amino Acid Sequence | ||||
| dc.subject.mesh | Brain - metabolism | ||||
| dc.subject.mesh | Chickens - metabolism | ||||
| dc.subject.mesh | Neuropeptides - genetics - metabolism | ||||
| dc.subject.mesh | Receptors, Neuropeptide - genetics - metabolism | ||||
| dc.title | Identification of the receptors for prolactin-releasing peptide (PrRP) and Carassius RFamide peptide (C-RFa) in chickens | ||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong
- Sichuan University