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Article: Cell culture-adapted IBDV uses endocytosis for entry in DF-1 chicken embryonic fibroblasts

TitleCell culture-adapted IBDV uses endocytosis for entry in DF-1 chicken embryonic fibroblasts
Authors
KeywordsCalcium
Clathrin-independent endocytosis
IBDV
Macropinocytosis
Issue Date2012
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/virusres
Citation
Virus Research, 2012, v. 165 n. 1, p. 9-16 How to Cite?
AbstractAlthough membrane perforation was suggested as the means of penetration mediated by IBDV, the cellular mechanism being hijacked to facilitate its entry is largely unknown. In this study, the entry pathway of cell culture adapted IBDV (caIBDV) was characterized in DF-1 chicken embryonic fibroblasts. We observed that the entry of caIBDV was inhibited by bafilomycin A1 and CaEGTA which interfere with the function of vacuolar H +-ATPase (V-ATPase) and retain endosomal Ca 2+. This result suggests that the intact caIBDV particle was transported to the V-ATPase positive vesicles for uncoating and implicates an essential role of endocytosis during the viral entry. The IBDV-mediated endocytosis was demonstrated to be clathrin-independent. Instead, the entry of caIBDV in DF-1 was reduced under the inhibitions or depletions of lipid raft, c-Src tyrosine kinase, dynamin and actin polymerization. In summary, this study confirmed the role of endocytosis in caIBDV entry and characterized the route of its endocytosis. © 2011 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/153209
ISSN
2015 Impact Factor: 2.526
2015 SCImago Journal Rankings: 1.259
ISI Accession Number ID
Funding AgencyGrant Number
RGC GRFHKU 771310M
Funding Information:

This project is partly supported by RGC GRF: HKU 771310M.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorYip, CWen_HK
dc.contributor.authorHon, CCen_HK
dc.contributor.authorZeng, Fen_HK
dc.contributor.authorLeung, FCCen_HK
dc.date.accessioned2012-07-16T09:59:50Z-
dc.date.available2012-07-16T09:59:50Z-
dc.date.issued2012en_HK
dc.identifier.citationVirus Research, 2012, v. 165 n. 1, p. 9-16en_HK
dc.identifier.issn0168-1702en_HK
dc.identifier.urihttp://hdl.handle.net/10722/153209-
dc.description.abstractAlthough membrane perforation was suggested as the means of penetration mediated by IBDV, the cellular mechanism being hijacked to facilitate its entry is largely unknown. In this study, the entry pathway of cell culture adapted IBDV (caIBDV) was characterized in DF-1 chicken embryonic fibroblasts. We observed that the entry of caIBDV was inhibited by bafilomycin A1 and CaEGTA which interfere with the function of vacuolar H +-ATPase (V-ATPase) and retain endosomal Ca 2+. This result suggests that the intact caIBDV particle was transported to the V-ATPase positive vesicles for uncoating and implicates an essential role of endocytosis during the viral entry. The IBDV-mediated endocytosis was demonstrated to be clathrin-independent. Instead, the entry of caIBDV in DF-1 was reduced under the inhibitions or depletions of lipid raft, c-Src tyrosine kinase, dynamin and actin polymerization. In summary, this study confirmed the role of endocytosis in caIBDV entry and characterized the route of its endocytosis. © 2011 Elsevier B.V.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/virusresen_HK
dc.relation.ispartofVirus Researchen_HK
dc.subjectCalciumen_HK
dc.subjectClathrin-independent endocytosisen_HK
dc.subjectIBDVen_HK
dc.subjectMacropinocytosisen_HK
dc.subject.meshBirnaviridae Infections - physiopathology - veterinary - virology-
dc.subject.meshFibroblasts - physiology - virology-
dc.subject.meshInfectious bursal disease virus - genetics - physiology-
dc.subject.meshPoultry Diseases - physiopathology - virology-
dc.subject.meshTransport Vesicles - virology-
dc.titleCell culture-adapted IBDV uses endocytosis for entry in DF-1 chicken embryonic fibroblastsen_HK
dc.typeArticleen_HK
dc.identifier.emailLeung, FCC: fcleung@hkucc.hku.hken_HK
dc.identifier.authorityLeung, FCC=rp00731en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.virusres.2011.12.016en_HK
dc.identifier.pmid22230315-
dc.identifier.scopuseid_2-s2.0-84858864021en_HK
dc.identifier.hkuros200939en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84858864021&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume165en_HK
dc.identifier.issue1en_HK
dc.identifier.spage9en_HK
dc.identifier.epage16en_HK
dc.identifier.isiWOS:000302976800002-
dc.publisher.placeNetherlandsen_HK
dc.relation.projectIBDV, heat shock protein 90, cell culture adaptation, VP2, endocytic pathway-
dc.identifier.scopusauthoridYip, CW=54685625700en_HK
dc.identifier.scopusauthoridHon, CC=7003617137en_HK
dc.identifier.scopusauthoridZeng, F=7202911544en_HK
dc.identifier.scopusauthoridLeung, FCC=7103078633en_HK
dc.identifier.citeulike10191298-

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