File Download
  Links for fulltext
     (May Require Subscription)
  • Find via Find It@HKUL
Supplementary

Conference Paper: Effect of luteolin on endothelium-dependent relaxation is different between male and female rat mesenteric arteries: relationship with endothelium-derived hyperpolarizing factor and cyclooxygenase pathways

TitleEffect of luteolin on endothelium-dependent relaxation is different between male and female rat mesenteric arteries: relationship with endothelium-derived hyperpolarizing factor and cyclooxygenase pathways
Authors
KeywordsCardiovascular disease
Issue Date2011
PublisherHong Kong College of Cardiology. The Journal's web site is located at http://www.hkcchk.com/journals.php#3
Citation
The 15th Annual Scientific Meeting of the Institute of Cardiovascular Science and Medicine (ICSM 2011), Hong Kong, 17 September 2011. In Journal of the Hong Kong College of Cardiology, 2011, v. 19 n. 2, p. 70, abstract no. OC5 How to Cite?
AbstractBACKGROUND: Luteolin is a non-steroidal polyphenolic plant metabolite which has similar structure with estrogen. The present study focused on whether or not luteolin produced gender-specific effects on relaxations that were mediated by different endothelium-derived factors. METHODS: Mesenteric arteries were isolated from 45-50 weeks old male and female Sprague Dawley rats, and incubated in the organ chambers filled with Kreb’s solution and bubbled with 95% O2 and 5% CO2. Endothelium-dependent relaxations mediated by nitric oxide (NO), endothelium-derived hyperpolarizing factors (EDHF) and cyclooxygenase (COX) products were studied. RESULTS: Our data showed that luteolin (10-5 M) enhanced endothelium-dependent relaxation in general and NO-mediated relaxations in both male and female rat mesenteric arteries, and the degree of these enhancements did not differ between the two genders. In the presence of indomethacin (10-6 M, COX inhibitor) and L-NAME (10-5 M, NO synthase inhibitor), EDHF-mediated relaxation were enhanced by luteolin in a greater manner in male than that in female. This phenomenon was also observed in relaxation mediated by intermediate conductance calcium-activated potassium channel (IKCa) and small calcium-activated potassium channel (SKCa). However, in the presence of L-NAME, UCL 1684 (10-6 M, SKCa antagonist) and TRAM-34 (10-6 M, IKCa antagonist), luteolin produced a smaller enhancement of COX-mediated relaxation in male than that in female. CONCLUSIONS: In conclusion, luteolin selectively enhanced EHDF signaling pathways and this enhancement was greater in male than in female. On the other hand, luteolin caused greater activation of COX pathway in female than in male. Further study will focus on the expression of COX and potassium channels in both male and female rats.
DescriptionOral Communications
This journal issue contain abstracts of the 15th ICSM Annual Meeting 2011
Persistent Identifierhttp://hdl.handle.net/10722/153134
ISSN
2015 SCImago Journal Rankings: 0.102

 

DC FieldValueLanguage
dc.contributor.authorZhang, Yen_US
dc.contributor.authorLeung, SWSen_US
dc.contributor.authorMan, RYKen_US
dc.date.accessioned2012-07-16T09:57:49Z-
dc.date.available2012-07-16T09:57:49Z-
dc.date.issued2011en_US
dc.identifier.citationThe 15th Annual Scientific Meeting of the Institute of Cardiovascular Science and Medicine (ICSM 2011), Hong Kong, 17 September 2011. In Journal of the Hong Kong College of Cardiology, 2011, v. 19 n. 2, p. 70, abstract no. OC5en_US
dc.identifier.issn1027-7811-
dc.identifier.urihttp://hdl.handle.net/10722/153134-
dc.descriptionOral Communications-
dc.descriptionThis journal issue contain abstracts of the 15th ICSM Annual Meeting 2011-
dc.description.abstractBACKGROUND: Luteolin is a non-steroidal polyphenolic plant metabolite which has similar structure with estrogen. The present study focused on whether or not luteolin produced gender-specific effects on relaxations that were mediated by different endothelium-derived factors. METHODS: Mesenteric arteries were isolated from 45-50 weeks old male and female Sprague Dawley rats, and incubated in the organ chambers filled with Kreb’s solution and bubbled with 95% O2 and 5% CO2. Endothelium-dependent relaxations mediated by nitric oxide (NO), endothelium-derived hyperpolarizing factors (EDHF) and cyclooxygenase (COX) products were studied. RESULTS: Our data showed that luteolin (10-5 M) enhanced endothelium-dependent relaxation in general and NO-mediated relaxations in both male and female rat mesenteric arteries, and the degree of these enhancements did not differ between the two genders. In the presence of indomethacin (10-6 M, COX inhibitor) and L-NAME (10-5 M, NO synthase inhibitor), EDHF-mediated relaxation were enhanced by luteolin in a greater manner in male than that in female. This phenomenon was also observed in relaxation mediated by intermediate conductance calcium-activated potassium channel (IKCa) and small calcium-activated potassium channel (SKCa). However, in the presence of L-NAME, UCL 1684 (10-6 M, SKCa antagonist) and TRAM-34 (10-6 M, IKCa antagonist), luteolin produced a smaller enhancement of COX-mediated relaxation in male than that in female. CONCLUSIONS: In conclusion, luteolin selectively enhanced EHDF signaling pathways and this enhancement was greater in male than in female. On the other hand, luteolin caused greater activation of COX pathway in female than in male. Further study will focus on the expression of COX and potassium channels in both male and female rats.-
dc.languageengen_US
dc.publisherHong Kong College of Cardiology. The Journal's web site is located at http://www.hkcchk.com/journals.php#3-
dc.relation.ispartofJournal of the Hong Kong College of Cardiologyen_US
dc.subjectCardiovascular disease-
dc.titleEffect of luteolin on endothelium-dependent relaxation is different between male and female rat mesenteric arteries: relationship with endothelium-derived hyperpolarizing factor and cyclooxygenase pathwaysen_US
dc.typeConference_Paperen_US
dc.identifier.emailLeung, SWS: swsleung@hku.hken_US
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_US
dc.identifier.authorityLeung, SWS=rp00235en_US
dc.identifier.authorityMan, RYK=rp00236en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros200780en_US
dc.identifier.volume19-
dc.identifier.issue2-
dc.identifier.spage70-
dc.identifier.epage70-
dc.publisher.placeHong Kong-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats