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- Publisher Website: 10.1007/s12640-012-9319-6
- Scopus: eid_2-s2.0-84870299180
- PMID: 22451226
- WOS: WOS:000308966400009
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Article: Z-ligustilide potentiates the cytotoxicity of dopamine in rat dopaminergic PC12 cells
Title | Z-ligustilide potentiates the cytotoxicity of dopamine in rat dopaminergic PC12 cells |
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Authors | |
Keywords | Cytotoxicity Dopamine Parkinson's disease Reactive oxygen species Reduced glutathione |
Issue Date | 2012 |
Publisher | Springer New York LLC. The Journal's web site is located at http://www.springerlink.com/content/1029-8428 |
Citation | Neurotoxicity Research, 2012, v. 22 n. 4, p. 345-354 How to Cite? |
Abstract | Dopamine toxicity is an ongoing controversy surrounding the use of levadopa (L-Dopa) in the therapy of Parkinson's disease. The initial objective of this study was to investigate the potential of neuroprotective botanicals such as Z-ligustilide in reducing the cytotoxicity of dopamine. We surprisingly found that Z-ligustilide potentiated dopamine toxicity in a dopaminergic cell specific manner. Using rat dopaminergic cell line PC12 as a model, we demonstrated that dopamine and Z-ligustilide in combination profoundly induced cell death, although these drugs alone, to a lesser extent, affected the cell viability in a concentration-dependent manner. The synergistic cytotoxicity of dopamine and Z-ligustilide is likely mediated via apoptosis, characterized by DNA fragmentation and chromatin shrinking after 12 h incubation. By measuring the intracellular reactive oxygen species (ROS) and reduced glutathione (GSH), Z-ligustilide and dopamine in combination dramatically enhanced the ROS formation and further depleted reduced GSH, whereas these drugs alone showed much less activity. Importantly, the synergistic cytotoxicity of dopamine and Z-ligustilide could be largely prevented by thiol-containing antioxidant N-acetylcysteine and GSH other than vitamin C and Trolox. Since the cytotoxicity of Z-ligustilide was not reported previously, the results of this study should raise public concerns over the potential risk associated with the combined use of herbal medicines containing Z-ligustilide with L-Dopa in the therapy of Parkinson's disease. |
Persistent Identifier | http://hdl.handle.net/10722/152802 |
ISSN | 2021 Impact Factor: 3.978 2020 SCImago Journal Rankings: 0.923 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Qi, H | en_HK |
dc.contributor.author | Zhao, J | en_HK |
dc.contributor.author | Han, Y | en_HK |
dc.contributor.author | Lau, ASY | en_HK |
dc.contributor.author | Rong, J | en_HK |
dc.date.accessioned | 2012-07-16T09:48:41Z | - |
dc.date.available | 2012-07-16T09:48:41Z | - |
dc.date.issued | 2012 | en_HK |
dc.identifier.citation | Neurotoxicity Research, 2012, v. 22 n. 4, p. 345-354 | en_HK |
dc.identifier.issn | 1029-8428 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/152802 | - |
dc.description.abstract | Dopamine toxicity is an ongoing controversy surrounding the use of levadopa (L-Dopa) in the therapy of Parkinson's disease. The initial objective of this study was to investigate the potential of neuroprotective botanicals such as Z-ligustilide in reducing the cytotoxicity of dopamine. We surprisingly found that Z-ligustilide potentiated dopamine toxicity in a dopaminergic cell specific manner. Using rat dopaminergic cell line PC12 as a model, we demonstrated that dopamine and Z-ligustilide in combination profoundly induced cell death, although these drugs alone, to a lesser extent, affected the cell viability in a concentration-dependent manner. The synergistic cytotoxicity of dopamine and Z-ligustilide is likely mediated via apoptosis, characterized by DNA fragmentation and chromatin shrinking after 12 h incubation. By measuring the intracellular reactive oxygen species (ROS) and reduced glutathione (GSH), Z-ligustilide and dopamine in combination dramatically enhanced the ROS formation and further depleted reduced GSH, whereas these drugs alone showed much less activity. Importantly, the synergistic cytotoxicity of dopamine and Z-ligustilide could be largely prevented by thiol-containing antioxidant N-acetylcysteine and GSH other than vitamin C and Trolox. Since the cytotoxicity of Z-ligustilide was not reported previously, the results of this study should raise public concerns over the potential risk associated with the combined use of herbal medicines containing Z-ligustilide with L-Dopa in the therapy of Parkinson's disease. | en_HK |
dc.language | eng | en_US |
dc.publisher | Springer New York LLC. The Journal's web site is located at http://www.springerlink.com/content/1029-8428 | en_HK |
dc.relation.ispartof | Neurotoxicity Research | en_HK |
dc.rights | The original publication is available at www.springerlink.com | - |
dc.subject | Cytotoxicity | en_HK |
dc.subject | Dopamine | en_HK |
dc.subject | Parkinson's disease | en_HK |
dc.subject | Reactive oxygen species | en_HK |
dc.subject | Reduced glutathione | en_HK |
dc.title | Z-ligustilide potentiates the cytotoxicity of dopamine in rat dopaminergic PC12 cells | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Lau, ASY: asylau@hku.hk | en_HK |
dc.identifier.email | Rong, J: jrong@hku.hk | en_HK |
dc.identifier.authority | Lau, ASY=rp00474 | en_HK |
dc.identifier.authority | Rong, J=rp00515 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1007/s12640-012-9319-6 | en_HK |
dc.identifier.pmid | 22451226 | - |
dc.identifier.scopus | eid_2-s2.0-84870299180 | en_HK |
dc.identifier.hkuros | 203763 | en_US |
dc.identifier.volume | 22 | - |
dc.identifier.issue | 4 | - |
dc.identifier.spage | 345 | en_HK |
dc.identifier.epage | 354 | en_HK |
dc.identifier.eissn | 1476-3524 | - |
dc.identifier.isi | WOS:000308966400009 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Rong, J=7005980047 | en_HK |
dc.identifier.scopusauthorid | Lau, ASY=7202626202 | en_HK |
dc.identifier.scopusauthorid | Han, Y=8527680500 | en_HK |
dc.identifier.scopusauthorid | Zhao, J=55122766600 | en_HK |
dc.identifier.scopusauthorid | Qi, H=35367105300 | en_HK |
dc.identifier.issnl | 1029-8428 | - |