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Article: Brazilian green propolis and its constituent, Artepillin C inhibits allogeneic activated human CD4 T cells expansion and activation

TitleBrazilian green propolis and its constituent, Artepillin C inhibits allogeneic activated human CD4 T cells expansion and activation
Authors
KeywordsArtepillin c
Carboxyfluorescein diacetate succinimidyl ester
Gamma interferon
Interleukin 17
Interleukin 2
Issue Date2011
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm
Citation
Journal of Ethnopharmacology, 2011, v. 138 n. 2, p. 463-471 How to Cite?
AbstractETHNOPHARMACOLOGICAL RELEVANCE: Propolis has long been used as a popular folk medicine by various ethnic groups due to its wide spectrum of alleged biological and pharmaceutical properties including anti-microbial, anti-cancer and anti-inflammatory functions. All these can be linked to the modulation of immune function. Therefore, it will be relevant for us to find out whether there is any novel compound that can account for such action and the mechanism involved. AIM OF THE STUDY: We investigated the immune modulating effect of Brazilian green propolis (PBrazil) and its constituent Artepillin C (Art-C) by using mixed leukocytes reaction. MATERIALS AND METHODS: The cytotoxic effect of Art-C on non-tumorigenic human liver cell line miHA and non-tumorigenic human kidney cell line HK-2 as well as human peripheral blood mononuclear cells (PBMCs) were measured by XTT cell proliferation assay. The effect of PBrazil and Art-C on T cell proliferation and activation were determined by using carboxyfluorescein succinimidyl ester (CFSE) and by CD25 expression, respectively. Cytokines including tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), interleukins such as IL-2, IL-17 were measured by intracellular cytokine staining and IL-10 was measured by ELISA. The effect of PBrazil and Art-C on regulatory T cells (Treg) induction was determined by the Foxp3 expression. The apoptotic effect of these compounds on CFSE labeled alloreactive T cells was measured by using Annexin V. RESULTS: Using mixed leukocytes reaction we demonstrated for the first time that both Art-C and PBrazil significantly inhibited the alloreactive CD4 T cell proliferation, activation, and suppressed the expressions of IL-2, IFN-gamma and IL-17 in these alloreactive CD4 T cells. The inhibitions of Art-C and PBrazil on CD4 T cells were not due to direct cytotoxic effect on PBMC or inducing regulatory T cells differentiation. Both Art-C and PBrazil were found to selectively induce apoptosis in proliferating T cells. The anti-proliferative effect of Art-C and PBrazil were reversible and were also applied to the activated T cells. CONCLUSIONS: In conclusion, our results indicated that Art-C and PBrazil can suppress alloreactive CD4 T cell responses in vitro, suggesting that Art-C could be used as a potential immunosuppressant, either solely or as adjunct agent in treating graft versus host disease.
Persistent Identifierhttp://hdl.handle.net/10722/152793
ISSN
2015 Impact Factor: 3.055
2015 SCImago Journal Rankings: 1.156
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCheung, KWen_HK
dc.contributor.authorSze, DMYen_HK
dc.contributor.authorChan, WKen_HK
dc.contributor.authorDeng, RXen_HK
dc.contributor.authorTu, Wen_HK
dc.contributor.authorChan, GCFen_HK
dc.date.accessioned2012-07-16T09:48:37Z-
dc.date.available2012-07-16T09:48:37Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal of Ethnopharmacology, 2011, v. 138 n. 2, p. 463-471en_HK
dc.identifier.issn0378-8741en_HK
dc.identifier.urihttp://hdl.handle.net/10722/152793-
dc.description.abstractETHNOPHARMACOLOGICAL RELEVANCE: Propolis has long been used as a popular folk medicine by various ethnic groups due to its wide spectrum of alleged biological and pharmaceutical properties including anti-microbial, anti-cancer and anti-inflammatory functions. All these can be linked to the modulation of immune function. Therefore, it will be relevant for us to find out whether there is any novel compound that can account for such action and the mechanism involved. AIM OF THE STUDY: We investigated the immune modulating effect of Brazilian green propolis (PBrazil) and its constituent Artepillin C (Art-C) by using mixed leukocytes reaction. MATERIALS AND METHODS: The cytotoxic effect of Art-C on non-tumorigenic human liver cell line miHA and non-tumorigenic human kidney cell line HK-2 as well as human peripheral blood mononuclear cells (PBMCs) were measured by XTT cell proliferation assay. The effect of PBrazil and Art-C on T cell proliferation and activation were determined by using carboxyfluorescein succinimidyl ester (CFSE) and by CD25 expression, respectively. Cytokines including tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), interleukins such as IL-2, IL-17 were measured by intracellular cytokine staining and IL-10 was measured by ELISA. The effect of PBrazil and Art-C on regulatory T cells (Treg) induction was determined by the Foxp3 expression. The apoptotic effect of these compounds on CFSE labeled alloreactive T cells was measured by using Annexin V. RESULTS: Using mixed leukocytes reaction we demonstrated for the first time that both Art-C and PBrazil significantly inhibited the alloreactive CD4 T cell proliferation, activation, and suppressed the expressions of IL-2, IFN-gamma and IL-17 in these alloreactive CD4 T cells. The inhibitions of Art-C and PBrazil on CD4 T cells were not due to direct cytotoxic effect on PBMC or inducing regulatory T cells differentiation. Both Art-C and PBrazil were found to selectively induce apoptosis in proliferating T cells. The anti-proliferative effect of Art-C and PBrazil were reversible and were also applied to the activated T cells. CONCLUSIONS: In conclusion, our results indicated that Art-C and PBrazil can suppress alloreactive CD4 T cell responses in vitro, suggesting that Art-C could be used as a potential immunosuppressant, either solely or as adjunct agent in treating graft versus host disease.en_HK
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharmen_HK
dc.relation.ispartofJournal of Ethnopharmacologyen_HK
dc.subjectArtepillin cen_HK
dc.subjectCarboxyfluorescein diacetate succinimidyl esteren_HK
dc.subjectGamma interferonen_HK
dc.subjectInterleukin 17en_HK
dc.subjectInterleukin 2en_HK
dc.titleBrazilian green propolis and its constituent, Artepillin C inhibits allogeneic activated human CD4 T cells expansion and activationen_HK
dc.typeArticleen_HK
dc.identifier.emailCheung, KW: bkwc@hku.hken_HK
dc.identifier.emailChan, WK: wingkc@graduate.hku.hken_HK
dc.identifier.emailTu, W: wwtu@hku.hk-
dc.identifier.emailChan, GCF: gcfchan@hku.hk-
dc.identifier.authorityTu, W=rp00416en_HK
dc.identifier.authorityChan, GCF=rp00431en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jep.2011.09.031en_HK
dc.identifier.pmid21964192-
dc.identifier.scopuseid_2-s2.0-81255158086en_HK
dc.identifier.hkuros200728en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-81255158086&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume138en_HK
dc.identifier.issue2en_HK
dc.identifier.spage463en_HK
dc.identifier.epage471en_HK
dc.identifier.isiWOS:000298026400024-
dc.publisher.placeIrelanden_HK
dc.identifier.scopusauthoridChan, GCF=16160154400en_HK
dc.identifier.scopusauthoridTu, W=7006479236en_HK
dc.identifier.scopusauthoridDeng, RX=35209695100en_HK
dc.identifier.scopusauthoridChan, WK=35080149500en_HK
dc.identifier.scopusauthoridSze, DMY=7005428465en_HK
dc.identifier.scopusauthoridCheung, KW=54392710800en_HK
dc.identifier.citeulike9824249-

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