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Article: Amino acid residues 253 and 591 of the PB2 protein of avian influenza virus A H9N2 contribute to mammalian pathogenesis

TitleAmino acid residues 253 and 591 of the PB2 protein of avian influenza virus A H9N2 contribute to mammalian pathogenesis
Authors
Mok, CKPYen, HLYu, MYMYuen, KMSia, SFChan, MCWQin, GTu, WWPeiris, JSM
Issue Date2011
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
Citation
Journal Of Virology, 2011, v. 85 n. 18, p. 9641-9645 How to Cite?
DOI: http://dx.doi.org/10.1128/JVI.00702-11
AbstractWe investigated the tropism, host responses, and virulence of two variants of A/Quail/Hong Kong/G1/1997 (H9N2) (H9N2/G1) with D253N and Q591K in the PB2 protein in primary human macrophages and bronchial epithelium in vitro and in mice in vivo. Virus with PB2 D253N and Q591K had greater polymerase activity in minireplicon assays, induced more tumor necrosis factor alpha (TNF-α) in human macrophages, replicated better in differentiated normal human bronchial epithelial (NHBE) cells, and was more pathogenic for mice. Taken together, our studies help define the viral genetic determinants that contribute to pathogenicity of H9N2 viruses. © 2011, American Society for Microbiology.
Persistent Identifierhttp://hdl.handle.net/10722/152775
ISSN
0022-538X
2023 Impact Factor: 4.0
2023 SCImago Journal Rankings: 1.378
PubMed Central ID
PMC3165745
ISI Accession Number ID
WOS:000293956400037
Funding AgencyGrant Number
National Institute of Allergy and Infectious Diseases (NIAID)HHSN26600700005C
Area of Excellence of the University Grants Commission of the Hong Kong SAR GovernmentAoE/M-12/06
Research Grants Council, Hong Kong SAR GovernmentHKU 7612/08 M
Funding Information:

This research was supported in part by the National Institute of Allergy and Infectious Diseases (NIAID) contract HHSN26600700005C, the Area of Excellence Scheme of the University Grants Commission (grant AoE/M-12/06) of the Hong Kong SAR Government, and the General Research Fund (HKU 7612/08 M), Research Grants Council, Hong Kong SAR Government.

References
References in Scopus
Grants
Control of Pandemic and Inter-pandemic Influenza

 

DC FieldValueLanguage
dc.contributor.authorMok, CKPen_HK
dc.contributor.authorYen, HLen_HK
dc.contributor.authorYu, MYMen_HK
dc.contributor.authorYuen, KMen_HK
dc.contributor.authorSia, SFen_HK
dc.contributor.authorChan, MCWen_HK
dc.contributor.authorQin, Gen_HK
dc.contributor.authorTu, WWen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.date.accessioned2012-07-16T09:48:01Z-
dc.date.available2012-07-16T09:48:01Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of Virology, 2011, v. 85 n. 18, p. 9641-9645en_HK
dc.identifier.issn0022-538Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/152775-
dc.description.abstractWe investigated the tropism, host responses, and virulence of two variants of A/Quail/Hong Kong/G1/1997 (H9N2) (H9N2/G1) with D253N and Q591K in the PB2 protein in primary human macrophages and bronchial epithelium in vitro and in mice in vivo. Virus with PB2 D253N and Q591K had greater polymerase activity in minireplicon assays, induced more tumor necrosis factor alpha (TNF-α) in human macrophages, replicated better in differentiated normal human bronchial epithelial (NHBE) cells, and was more pathogenic for mice. Taken together, our studies help define the viral genetic determinants that contribute to pathogenicity of H9N2 viruses. © 2011, American Society for Microbiology.en_HK
dc.languageengen_US
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/en_HK
dc.relation.ispartofJournal of Virologyen_HK
dc.rightsJournal of Virology. Copyright © American Society for Microbiology.-
dc.rightsCopyright © American Society for Microbiology, [Journal of Virology, 2011, v. 85 n. 18, p. 9641-9645]-
dc.subject.meshInfluenza A Virus, H9N2 Subtype - genetics - pathogenicity-
dc.subject.meshRNA Replicase - genetics - metabolism-
dc.subject.meshViral Proteins - genetics - metabolism-
dc.subject.meshViral Tropism-
dc.subject.meshVirulence Factors - genetics - metabolism-
dc.titleAmino acid residues 253 and 591 of the PB2 protein of avian influenza virus A H9N2 contribute to mammalian pathogenesisen_HK
dc.typeArticleen_HK
dc.identifier.emailMok, CKP: ch02mkp@hkucc.hku.hken_HK
dc.identifier.emailYen, HL: hyen@hku.hken_HK
dc.identifier.emailChan, MCW: mchan@hkucc.hku.hken_HK
dc.identifier.emailTu, WW: wwtu@hkucc.hku.hken_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.authorityMok, CKP=rp01805en_HK
dc.identifier.authorityYen, HL=rp00304en_HK
dc.identifier.authorityChan, MCW=rp00420en_HK
dc.identifier.authorityTu, WW=rp00416en_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.description.naturelink_to_OA_fulltexten_HK
dc.identifier.doi10.1128/JVI.00702-11en_HK
dc.identifier.pmid21734052-
dc.identifier.pmcidPMC3165745-
dc.identifier.scopuseid_2-s2.0-80052464359en_HK
dc.identifier.hkuros200731en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80052464359&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume85en_HK
dc.identifier.issue18en_HK
dc.identifier.spage9641en_HK
dc.identifier.epage9645en_HK
dc.identifier.isiWOS:000293956400037-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectControl of Pandemic and Inter-pandemic Influenza-
dc.identifier.scopusauthoridMok, CKP=36159732100en_HK
dc.identifier.scopusauthoridYen, HL=7102476668en_HK
dc.identifier.scopusauthoridYu, MYM=53868594200en_HK
dc.identifier.scopusauthoridYuen, KM=35301205900en_HK
dc.identifier.scopusauthoridSia, SF=8574447900en_HK
dc.identifier.scopusauthoridChan, MCW=26654715500en_HK
dc.identifier.scopusauthoridQin, G=35085420900en_HK
dc.identifier.scopusauthoridTu, WW=7006479236en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.issnl0022-538X-

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