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Article: Dissemination of pHK01-like incompatibility group IncFII plasmids encoding CTX-M-14 in Escherichia coli from human and animal sources

TitleDissemination of pHK01-like incompatibility group IncFII plasmids encoding CTX-M-14 in Escherichia coli from human and animal sources
Authors
KeywordsChicken
Extended-spectrum beta-lactamases
Pets
Pigs
Plasmid
Issue Date2012
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/vetmic
Citation
Veterinary Microbiology, 2012, v. 158 n. 1-2, p. 172-179 How to Cite?
AbstractFew studies have compared CTX-M encoding plasmids identified in different ecological sources. This study aimed to analyze and compare the molecular epidemiology of plasmids encoding CTX-M-14 among strains from humans and animals. The CTX-M-14 encoding plasmids in 160 Escherichia coli isolates from animal faecal (14 pigs, 16 chickens, 12 cats, 8 cattle, 5 dogs and 3 rodents), human faecal (45 adults and 20 children) and human urine (37 adults) sources in 2002-2010 were characterized by molecular methods. The replicon types of the CTX-M-14 encoding plasmids were IncFII (n=61), I1-Iγ (n=24), other F types (n=23), B/O (n=10), K (n=6), N (n=3), A/C (n=1), HI1 (n=1), HI2 (n=1) and nontypeable (n=30). The genetic environment, ISEcp1 - bla CTX-M-14 - IS903 was found in 89.7% (52/58), 87.7% (57/65) and 86.5% (32/37) of the animal faecal, human faecal and human urine isolates, respectively. Subtyping of the 61 IncFII incompatibility group plasmids by replicon sequence typing, plasmid PCR-restriction fragment length polymorphism and marker genes (yac, malB, eitA/eitC and parB/A) profiles showed that 31% (18/58), 30.6% (20/65) and 37.8% (14/37) of the plasmids originating from animal faecal, human faecal and human urine isolates, respectively, were pHK01-like. These 52 pHK01-like plasmids originated from diverse human (20 faecal isolates from 2002, 2007 to 2008, 14 urinary isolates from 2004) and animal (all faecal, 1 cattle, 1 chicken, 5 pigs, 9 cats, 1 dog, 1 rodent from 2008 to 2010) sources. In conclusion, this study highlights the importance of the IncFII group, pHK01-like plasmids in the dissemination of CTX-M-14 among isolates from diverse sources. © 2012 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/152771
ISSN
2015 Impact Factor: 2.564
2015 SCImago Journal Rankings: 1.381
ISI Accession Number ID
Funding AgencyGrant Number
Food and Health Bureau of the Government of the HKSAR
Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health
Funding Information:

This work was supported by grants from the Research Fund for the Control of Infectious Diseases (RFCID) of the Food and Health Bureau of the Government of the HKSAR, and the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health.

References

 

DC FieldValueLanguage
dc.contributor.authorHo, PLen_HK
dc.contributor.authorLo, WUen_HK
dc.contributor.authorYeung, MKen_HK
dc.contributor.authorLi, Zen_HK
dc.contributor.authorChan, Jen_HK
dc.contributor.authorChow, KHen_HK
dc.contributor.authorYam, WCen_HK
dc.contributor.authorTong, AHYen_HK
dc.contributor.authorBao, JYJen_HK
dc.contributor.authorLin, CHen_HK
dc.contributor.authorLok, Sen_HK
dc.contributor.authorChiu, SSen_HK
dc.date.accessioned2012-07-16T09:48:00Z-
dc.date.available2012-07-16T09:48:00Z-
dc.date.issued2012en_HK
dc.identifier.citationVeterinary Microbiology, 2012, v. 158 n. 1-2, p. 172-179en_HK
dc.identifier.issn0378-1135en_HK
dc.identifier.urihttp://hdl.handle.net/10722/152771-
dc.description.abstractFew studies have compared CTX-M encoding plasmids identified in different ecological sources. This study aimed to analyze and compare the molecular epidemiology of plasmids encoding CTX-M-14 among strains from humans and animals. The CTX-M-14 encoding plasmids in 160 Escherichia coli isolates from animal faecal (14 pigs, 16 chickens, 12 cats, 8 cattle, 5 dogs and 3 rodents), human faecal (45 adults and 20 children) and human urine (37 adults) sources in 2002-2010 were characterized by molecular methods. The replicon types of the CTX-M-14 encoding plasmids were IncFII (n=61), I1-Iγ (n=24), other F types (n=23), B/O (n=10), K (n=6), N (n=3), A/C (n=1), HI1 (n=1), HI2 (n=1) and nontypeable (n=30). The genetic environment, ISEcp1 - bla CTX-M-14 - IS903 was found in 89.7% (52/58), 87.7% (57/65) and 86.5% (32/37) of the animal faecal, human faecal and human urine isolates, respectively. Subtyping of the 61 IncFII incompatibility group plasmids by replicon sequence typing, plasmid PCR-restriction fragment length polymorphism and marker genes (yac, malB, eitA/eitC and parB/A) profiles showed that 31% (18/58), 30.6% (20/65) and 37.8% (14/37) of the plasmids originating from animal faecal, human faecal and human urine isolates, respectively, were pHK01-like. These 52 pHK01-like plasmids originated from diverse human (20 faecal isolates from 2002, 2007 to 2008, 14 urinary isolates from 2004) and animal (all faecal, 1 cattle, 1 chicken, 5 pigs, 9 cats, 1 dog, 1 rodent from 2008 to 2010) sources. In conclusion, this study highlights the importance of the IncFII group, pHK01-like plasmids in the dissemination of CTX-M-14 among isolates from diverse sources. © 2012 Elsevier B.V.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/vetmicen_HK
dc.relation.ispartofVeterinary Microbiologyen_HK
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Veterinary Microbiology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Veterinary Microbiology, 2012, v. 158 n. 1-2, p. 172-179. DOI: 10.1016/j.vetmic.2012.02.004-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectChickenen_HK
dc.subjectExtended-spectrum beta-lactamasesen_HK
dc.subjectPetsen_HK
dc.subjectPigsen_HK
dc.subjectPlasmiden_HK
dc.titleDissemination of pHK01-like incompatibility group IncFII plasmids encoding CTX-M-14 in Escherichia coli from human and animal sourcesen_HK
dc.typeArticleen_HK
dc.identifier.emailHo, PL: plho@hkucc.hku.hken_HK
dc.identifier.emailChow, KH: khchowb@hku.hken_HK
dc.identifier.emailYam, WC: wcyam@hkucc.hku.hken_HK
dc.identifier.emailLok, S: silok@genome.hku.hken_HK
dc.identifier.emailChiu, SS: ssschiu@hku.hken_HK
dc.identifier.authorityHo, PL=rp00406en_HK
dc.identifier.authorityChow, KH=rp00370en_HK
dc.identifier.authorityYam, WC=rp00313en_HK
dc.identifier.authorityLok, S=rp00271en_HK
dc.identifier.authorityChiu, SS=rp00421en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.vetmic.2012.02.004en_HK
dc.identifier.pmid22386670-
dc.identifier.scopuseid_2-s2.0-84862780331en_HK
dc.identifier.hkuros200719en_US
dc.identifier.hkuros219370-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84862780331&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume158en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage172en_HK
dc.identifier.epage179en_HK
dc.identifier.isiWOS:000305675400019-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridHo, PL=7402211363en_HK
dc.identifier.scopusauthoridLo, WU=35558916700en_HK
dc.identifier.scopusauthoridYeung, MK=37040000300en_HK
dc.identifier.scopusauthoridLi, Z=55039481400en_HK
dc.identifier.scopusauthoridChan, J=23090202000en_HK
dc.identifier.scopusauthoridChow, KH=7202180736en_HK
dc.identifier.scopusauthoridYam, WC=7004281720en_HK
dc.identifier.scopusauthoridTong, AHY=7103351716en_HK
dc.identifier.scopusauthoridBao, JYJ=45960893700en_HK
dc.identifier.scopusauthoridLin, CH=44761160100en_HK
dc.identifier.scopusauthoridLok, S=21035019900en_HK
dc.identifier.scopusauthoridChiu, SS=7202291500en_HK
dc.identifier.citeulike10423403-

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