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Article: Dissemination of pHK01-like incompatibility group IncFII plasmids encoding CTX-M-14 in Escherichia coli from human and animal sources
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TitleDissemination of pHK01-like incompatibility group IncFII plasmids encoding CTX-M-14 in Escherichia coli from human and animal sources
 
AuthorsHo, PL1 1
Lo, WU1
Yeung, MK1
Li, Z1
Chan, J1
Chow, KH1
Yam, WC1
Tong, AHY1
Bao, JYJ1
Lin, CH1
Lok, S1
Chiu, SS1
 
KeywordsChicken
Extended-spectrum beta-lactamases
Pets
Pigs
Plasmid
 
Issue Date2012
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/vetmic
 
CitationVeterinary Microbiology, 2012, v. 158 n. 1-2, p. 172-179 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.vetmic.2012.02.004
 
AbstractFew studies have compared CTX-M encoding plasmids identified in different ecological sources. This study aimed to analyze and compare the molecular epidemiology of plasmids encoding CTX-M-14 among strains from humans and animals. The CTX-M-14 encoding plasmids in 160 Escherichia coli isolates from animal faecal (14 pigs, 16 chickens, 12 cats, 8 cattle, 5 dogs and 3 rodents), human faecal (45 adults and 20 children) and human urine (37 adults) sources in 2002-2010 were characterized by molecular methods. The replicon types of the CTX-M-14 encoding plasmids were IncFII (n=61), I1-Iγ (n=24), other F types (n=23), B/O (n=10), K (n=6), N (n=3), A/C (n=1), HI1 (n=1), HI2 (n=1) and nontypeable (n=30). The genetic environment, ISEcp1 - bla CTX-M-14 - IS903 was found in 89.7% (52/58), 87.7% (57/65) and 86.5% (32/37) of the animal faecal, human faecal and human urine isolates, respectively. Subtyping of the 61 IncFII incompatibility group plasmids by replicon sequence typing, plasmid PCR-restriction fragment length polymorphism and marker genes (yac, malB, eitA/eitC and parB/A) profiles showed that 31% (18/58), 30.6% (20/65) and 37.8% (14/37) of the plasmids originating from animal faecal, human faecal and human urine isolates, respectively, were pHK01-like. These 52 pHK01-like plasmids originated from diverse human (20 faecal isolates from 2002, 2007 to 2008, 14 urinary isolates from 2004) and animal (all faecal, 1 cattle, 1 chicken, 5 pigs, 9 cats, 1 dog, 1 rodent from 2008 to 2010) sources. In conclusion, this study highlights the importance of the IncFII group, pHK01-like plasmids in the dissemination of CTX-M-14 among isolates from diverse sources. © 2012 Elsevier B.V.
 
ISSN0378-1135
2012 Impact Factor: 3.127
2012 SCImago Journal Rankings: 1.221
 
DOIhttp://dx.doi.org/10.1016/j.vetmic.2012.02.004
 
ISI Accession Number IDWOS:000305675400019
Funding AgencyGrant Number
Food and Health Bureau of the Government of the HKSAR
Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health
Funding Information:

This work was supported by grants from the Research Fund for the Control of Infectious Diseases (RFCID) of the Food and Health Bureau of the Government of the HKSAR, and the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorHo, PL
 
dc.contributor.authorLo, WU
 
dc.contributor.authorYeung, MK
 
dc.contributor.authorLi, Z
 
dc.contributor.authorChan, J
 
dc.contributor.authorChow, KH
 
dc.contributor.authorYam, WC
 
dc.contributor.authorTong, AHY
 
dc.contributor.authorBao, JYJ
 
dc.contributor.authorLin, CH
 
dc.contributor.authorLok, S
 
dc.contributor.authorChiu, SS
 
dc.date.accessioned2012-07-16T09:48:00Z
 
dc.date.available2012-07-16T09:48:00Z
 
dc.date.issued2012
 
dc.description.abstractFew studies have compared CTX-M encoding plasmids identified in different ecological sources. This study aimed to analyze and compare the molecular epidemiology of plasmids encoding CTX-M-14 among strains from humans and animals. The CTX-M-14 encoding plasmids in 160 Escherichia coli isolates from animal faecal (14 pigs, 16 chickens, 12 cats, 8 cattle, 5 dogs and 3 rodents), human faecal (45 adults and 20 children) and human urine (37 adults) sources in 2002-2010 were characterized by molecular methods. The replicon types of the CTX-M-14 encoding plasmids were IncFII (n=61), I1-Iγ (n=24), other F types (n=23), B/O (n=10), K (n=6), N (n=3), A/C (n=1), HI1 (n=1), HI2 (n=1) and nontypeable (n=30). The genetic environment, ISEcp1 - bla CTX-M-14 - IS903 was found in 89.7% (52/58), 87.7% (57/65) and 86.5% (32/37) of the animal faecal, human faecal and human urine isolates, respectively. Subtyping of the 61 IncFII incompatibility group plasmids by replicon sequence typing, plasmid PCR-restriction fragment length polymorphism and marker genes (yac, malB, eitA/eitC and parB/A) profiles showed that 31% (18/58), 30.6% (20/65) and 37.8% (14/37) of the plasmids originating from animal faecal, human faecal and human urine isolates, respectively, were pHK01-like. These 52 pHK01-like plasmids originated from diverse human (20 faecal isolates from 2002, 2007 to 2008, 14 urinary isolates from 2004) and animal (all faecal, 1 cattle, 1 chicken, 5 pigs, 9 cats, 1 dog, 1 rodent from 2008 to 2010) sources. In conclusion, this study highlights the importance of the IncFII group, pHK01-like plasmids in the dissemination of CTX-M-14 among isolates from diverse sources. © 2012 Elsevier B.V.
 
dc.description.naturepostprint
 
dc.identifier.citationVeterinary Microbiology, 2012, v. 158 n. 1-2, p. 172-179 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.vetmic.2012.02.004
 
dc.identifier.citeulike10423403
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.vetmic.2012.02.004
 
dc.identifier.epage179
 
dc.identifier.hkuros200719
 
dc.identifier.hkuros219370
 
dc.identifier.isiWOS:000305675400019
Funding AgencyGrant Number
Food and Health Bureau of the Government of the HKSAR
Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health
Funding Information:

This work was supported by grants from the Research Fund for the Control of Infectious Diseases (RFCID) of the Food and Health Bureau of the Government of the HKSAR, and the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health.

 
dc.identifier.issn0378-1135
2012 Impact Factor: 3.127
2012 SCImago Journal Rankings: 1.221
 
dc.identifier.issue1-2
 
dc.identifier.pmid22386670
 
dc.identifier.scopuseid_2-s2.0-84862780331
 
dc.identifier.spage172
 
dc.identifier.urihttp://hdl.handle.net/10722/152771
 
dc.identifier.volume158
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/vetmic
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofVeterinary Microbiology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Veterinary Microbiology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Veterinary Microbiology, 2012, v. 158 n. 1-2, p. 172-179. DOI: 10.1016/j.vetmic.2012.02.004
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subjectChicken
 
dc.subjectExtended-spectrum beta-lactamases
 
dc.subjectPets
 
dc.subjectPigs
 
dc.subjectPlasmid
 
dc.titleDissemination of pHK01-like incompatibility group IncFII plasmids encoding CTX-M-14 in Escherichia coli from human and animal sources
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong