Article: Dissemination of pHK01-like incompatibility group IncFII plasmids encoding CTX-M-14 in Escherichia coli from human and animal sources
| Title | Dissemination of pHK01-like incompatibility group IncFII plasmids encoding CTX-M-14 in Escherichia coli from human and animal sources | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Ho, PL1 Lo, WU1 Yeung, MK1 Li, Z1 Chan, J1 Chow, KH1 Yam, WC1 Tong, AHY1 Bao, JYJ1 Lin, CH1 Lok, S1 Chiu, SS1 | ||||||
| Keywords | Chicken Extended-spectrum beta-lactamases Pets Pigs Plasmid | ||||||
| Issue Date | 2012 | ||||||
| Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/vetmic | ||||||
| Citation | Veterinary Microbiology, 2012, v. 158 n. 1-2, p. 172-179 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.vetmic.2012.02.004 | ||||||
| Abstract | Few studies have compared CTX-M encoding plasmids identified in different ecological sources. This study aimed to analyze and compare the molecular epidemiology of plasmids encoding CTX-M-14 among strains from humans and animals. The CTX-M-14 encoding plasmids in 160 Escherichia coli isolates from animal faecal (14 pigs, 16 chickens, 12 cats, 8 cattle, 5 dogs and 3 rodents), human faecal (45 adults and 20 children) and human urine (37 adults) sources in 2002-2010 were characterized by molecular methods. The replicon types of the CTX-M-14 encoding plasmids were IncFII (n=61), I1-Iγ (n=24), other F types (n=23), B/O (n=10), K (n=6), N (n=3), A/C (n=1), HI1 (n=1), HI2 (n=1) and nontypeable (n=30). The genetic environment, ISEcp1 - bla CTX-M-14 - IS903 was found in 89.7% (52/58), 87.7% (57/65) and 86.5% (32/37) of the animal faecal, human faecal and human urine isolates, respectively. Subtyping of the 61 IncFII incompatibility group plasmids by replicon sequence typing, plasmid PCR-restriction fragment length polymorphism and marker genes (yac, malB, eitA/eitC and parB/A) profiles showed that 31% (18/58), 30.6% (20/65) and 37.8% (14/37) of the plasmids originating from animal faecal, human faecal and human urine isolates, respectively, were pHK01-like. These 52 pHK01-like plasmids originated from diverse human (20 faecal isolates from 2002, 2007 to 2008, 14 urinary isolates from 2004) and animal (all faecal, 1 cattle, 1 chicken, 5 pigs, 9 cats, 1 dog, 1 rodent from 2008 to 2010) sources. In conclusion, this study highlights the importance of the IncFII group, pHK01-like plasmids in the dissemination of CTX-M-14 among isolates from diverse sources. © 2012 Elsevier B.V. | ||||||
| ISSN | 0378-1135 2011 Impact Factor: 3.327 2011 SCImago Journal Rankings: 0.179 | ||||||
| DOI | http://dx.doi.org/10.1016/j.vetmic.2012.02.004 | ||||||
| ISI Accession Number ID | WOS:000305675400019
Funding Information: This work was supported by grants from the Research Fund for the Control of Infectious Diseases (RFCID) of the Food and Health Bureau of the Government of the HKSAR, and the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health. | ||||||
| References | References in Scopus |
| dc.contributor.author | Ho, PL | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Lo, WU | ||||||
| dc.contributor.author | Yeung, MK | ||||||
| dc.contributor.author | Li, Z | ||||||
| dc.contributor.author | Chan, J | ||||||
| dc.contributor.author | Chow, KH | ||||||
| dc.contributor.author | Yam, WC | ||||||
| dc.contributor.author | Tong, AHY | ||||||
| dc.contributor.author | Bao, JYJ | ||||||
| dc.contributor.author | Lin, CH | ||||||
| dc.contributor.author | Lok, S | ||||||
| dc.contributor.author | Chiu, SS | ||||||
| dc.date.accessioned | 2012-07-16T09:48:00Z | ||||||
| dc.date.available | 2012-07-16T09:48:00Z | ||||||
| dc.date.issued | 2012 | ||||||
| dc.description.abstract | Few studies have compared CTX-M encoding plasmids identified in different ecological sources. This study aimed to analyze and compare the molecular epidemiology of plasmids encoding CTX-M-14 among strains from humans and animals. The CTX-M-14 encoding plasmids in 160 Escherichia coli isolates from animal faecal (14 pigs, 16 chickens, 12 cats, 8 cattle, 5 dogs and 3 rodents), human faecal (45 adults and 20 children) and human urine (37 adults) sources in 2002-2010 were characterized by molecular methods. The replicon types of the CTX-M-14 encoding plasmids were IncFII (n=61), I1-Iγ (n=24), other F types (n=23), B/O (n=10), K (n=6), N (n=3), A/C (n=1), HI1 (n=1), HI2 (n=1) and nontypeable (n=30). The genetic environment, ISEcp1 - bla CTX-M-14 - IS903 was found in 89.7% (52/58), 87.7% (57/65) and 86.5% (32/37) of the animal faecal, human faecal and human urine isolates, respectively. Subtyping of the 61 IncFII incompatibility group plasmids by replicon sequence typing, plasmid PCR-restriction fragment length polymorphism and marker genes (yac, malB, eitA/eitC and parB/A) profiles showed that 31% (18/58), 30.6% (20/65) and 37.8% (14/37) of the plasmids originating from animal faecal, human faecal and human urine isolates, respectively, were pHK01-like. These 52 pHK01-like plasmids originated from diverse human (20 faecal isolates from 2002, 2007 to 2008, 14 urinary isolates from 2004) and animal (all faecal, 1 cattle, 1 chicken, 5 pigs, 9 cats, 1 dog, 1 rodent from 2008 to 2010) sources. In conclusion, this study highlights the importance of the IncFII group, pHK01-like plasmids in the dissemination of CTX-M-14 among isolates from diverse sources. © 2012 Elsevier B.V. | ||||||
| dc.description.nature | postprint | ||||||
| dc.identifier.citation | Veterinary Microbiology, 2012, v. 158 n. 1-2, p. 172-179 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.vetmic.2012.02.004 | ||||||
| dc.identifier.citeulike | 10423403 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1016/j.vetmic.2012.02.004 | ||||||
| dc.identifier.epage | 179 | ||||||
| dc.identifier.hkuros | 200719 | ||||||
| dc.identifier.isi | WOS:000305675400019
Funding Information: This work was supported by grants from the Research Fund for the Control of Infectious Diseases (RFCID) of the Food and Health Bureau of the Government of the HKSAR, and the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health. | ||||||
| dc.identifier.issn | 0378-1135 2011 Impact Factor: 3.327 2011 SCImago Journal Rankings: 0.179 | ||||||
| dc.identifier.issue | 1-2 | ||||||
| dc.identifier.pmid | 22386670 | ||||||
| dc.identifier.scopus | eid_2-s2.0-84862780331 | ||||||
| dc.identifier.spage | 172 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/152771 | ||||||
| dc.identifier.volume | 158 | ||||||
| dc.language | eng | ||||||
| dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/vetmic | ||||||
| dc.publisher.place | Netherlands | ||||||
| dc.relation.ispartof | Veterinary Microbiology | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.rights | NOTICE: this is the author’s version of a work that was accepted for publication in Veterinary Microbiology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Veterinary Microbiology, 2012, v. 158 n. 1-2, p. 172-179. DOI: 10.1016/j.vetmic.2012.02.004 | ||||||
| dc.rights | Creative Commons: Attribution 3.0 Hong Kong License | ||||||
| dc.subject | Chicken | ||||||
| dc.subject | Extended-spectrum beta-lactamases | ||||||
| dc.subject | Pets | ||||||
| dc.subject | Pigs | ||||||
| dc.subject | Plasmid | ||||||
| dc.title | Dissemination of pHK01-like incompatibility group IncFII plasmids encoding CTX-M-14 in Escherichia coli from human and animal sources | ||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong