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Article: The interaction of flavivirus M protein with light chain Tctex-1 of human dynein plays a role in late stages of virus replication

TitleThe interaction of flavivirus M protein with light chain Tctex-1 of human dynein plays a role in late stages of virus replication
Authors
Issue Date2011
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviro
Citation
Virology, 2011, v. 417 n. 2, p. 369-378 How to Cite?
AbstractThe role of the membrane protein (prM/M) in flavivirus life cycle remains unclear. Here, we identified a cellular interactor to the 40-residue-long ectodomain of prM/M (ectoM) using a yeast two-hybrid screen against a human cDNA library and GST pull-down assays. We showed that dynein light chain Tctex-1 interacts with the ectoM of dengue 1-4, West Nile, and Japanese encephalitis flaviviruses. No interaction was found with yellow fever and tick-borne flaviviruses. This interaction is highly specific since a single amino-acid change in the ectoM abrogates the interaction with Tctex-1. To understand the role of this interaction, silencing of Tctex-1 using siRNA was performed prior to infection. A significant decrease in progeny production was observed for dengue and West Nile viruses. Silencing Tctex-1 inhibited the production of recombinant dengue subviral particles (RSPs). Thus Tctex-1 may play a role in late stages of viral replication through its interaction with the membrane protein.
Persistent Identifierhttp://hdl.handle.net/10722/152637
ISSN
2015 Impact Factor: 3.2
2015 SCImago Journal Rankings: 1.805
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBrault, JBen_US
dc.contributor.authorKudelko, Men_US
dc.contributor.authorVidalain, POen_US
dc.contributor.authorTangy, Fen_US
dc.contributor.authorDespres, Pen_US
dc.contributor.authorPardigon, Nen_US
dc.date.accessioned2012-07-16T09:44:34Z-
dc.date.available2012-07-16T09:44:34Z-
dc.date.issued2011en_US
dc.identifier.citationVirology, 2011, v. 417 n. 2, p. 369-378en_US
dc.identifier.issn0042-6822-
dc.identifier.urihttp://hdl.handle.net/10722/152637-
dc.description.abstractThe role of the membrane protein (prM/M) in flavivirus life cycle remains unclear. Here, we identified a cellular interactor to the 40-residue-long ectodomain of prM/M (ectoM) using a yeast two-hybrid screen against a human cDNA library and GST pull-down assays. We showed that dynein light chain Tctex-1 interacts with the ectoM of dengue 1-4, West Nile, and Japanese encephalitis flaviviruses. No interaction was found with yellow fever and tick-borne flaviviruses. This interaction is highly specific since a single amino-acid change in the ectoM abrogates the interaction with Tctex-1. To understand the role of this interaction, silencing of Tctex-1 using siRNA was performed prior to infection. A significant decrease in progeny production was observed for dengue and West Nile viruses. Silencing Tctex-1 inhibited the production of recombinant dengue subviral particles (RSPs). Thus Tctex-1 may play a role in late stages of viral replication through its interaction with the membrane protein.-
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviro-
dc.relation.ispartofVirologyen_US
dc.subject.meshDengue Virus - physiology-
dc.subject.meshDyneins - genetics - metabolism-
dc.subject.meshEncephalitis Virus, Japanese - physiology-
dc.subject.meshProtein Interaction Mapping-
dc.subject.meshViral Envelope Proteins - metabolism-
dc.titleThe interaction of flavivirus M protein with light chain Tctex-1 of human dynein plays a role in late stages of virus replicationen_US
dc.typeArticleen_US
dc.identifier.emailKudelko, M: kudelko@hku.hken_US
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.virol.2011.06.022-
dc.identifier.pmid21767858-
dc.identifier.scopuseid_2-s2.0-80051942967-
dc.identifier.hkuros201707en_US
dc.identifier.volume417en_US
dc.identifier.issue2en_US
dc.identifier.spage369en_US
dc.identifier.epage378en_US
dc.identifier.isiWOS:000294578600016-
dc.publisher.placeUnited States-
dc.identifier.citeulike9631080-

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