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Article: Class II ADP-ribosylation factors are required for efficient secretion of dengue viruses
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TitleClass II ADP-ribosylation factors are required for efficient secretion of dengue viruses
 
AuthorsKudelko, M1
Brault, JB2
Kwok, K1
Li, MY1
Pardigon, N2
Peiris, JSM1
Bruzzone, R1 2
Despre, P2
Nal, B1 3
Wang, PG1
 
Issue Date2012
 
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
 
CitationJournal Of Biological Chemistry, 2012, v. 287 n. 1, p. 767-777 [How to Cite?]
DOI: http://dx.doi.org/10.1074/jbc.M111.270579
 
AbstractIdentification and characterization of virus-host interactions are very important steps toward a better understanding of the molecular mechanisms responsible for disease progression and pathogenesis. To date, very few cellular factors involved in the life cycle of flaviviruses, which are important human pathogens, have been described. In this study, we demonstrate a crucial role for class II Arf proteins (Arf4 and Arf5) in the dengue flavivirus life cycle. We show that simultaneous depletion of Arf4 and Arf5 blocks recombinant subviral particle secretion for all four dengue serotypes. Immunostaining analysis suggests that class II Arf proteins are required at an early pre-Golgi step for dengue virus secretion. Using a horseradish peroxidase protein fused to a signal peptide, we show that class II Arfs act specifically on dengue virus secretion without altering the secretion of proteins through the constitutive secretory pathway. Co-immunoprecipitation data demonstrate that the dengue prM glycoprotein interacts with class II Arf proteins but not through its C-terminal VXPX motif. Finally, experiments performed with replication-competent dengue and yellow fever viruses demonstrate that the depletion of class II Arfs inhibits virus secretion, thus confirming their implication in the virus life cycle, although data obtained with West Nile virus pointed out the differences in virus-host interactions among flaviviruses. Our findings shed new light on a molecular mechanism used by dengue viruses during the late stages of the life cycle and demonstrate a novel function for class II Arf proteins. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.
 
ISSN0021-9258
2013 Impact Factor: 4.600
 
DOIhttp://dx.doi.org/10.1074/jbc.M111.270579
 
ISI Accession Number IDWOS:000298682400074
Funding AgencyGrant Number
Research Fund for Control of Infectious Diseases of Hong KongRFCID 08070952
RFCID 10091312
BNP Paribas Corporate and Investment Banking
Funding Information:

This work was supported by Research Fund for Control of Infectious Diseases of Hong Kong Grants RFCID 08070952 and RFCID 10091312 and by a donation from BNP Paribas Corporate and Investment Banking.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorKudelko, M
 
dc.contributor.authorBrault, JB
 
dc.contributor.authorKwok, K
 
dc.contributor.authorLi, MY
 
dc.contributor.authorPardigon, N
 
dc.contributor.authorPeiris, JSM
 
dc.contributor.authorBruzzone, R
 
dc.contributor.authorDespre, P
 
dc.contributor.authorNal, B
 
dc.contributor.authorWang, PG
 
dc.date.accessioned2012-07-16T09:44:13Z
 
dc.date.available2012-07-16T09:44:13Z
 
dc.date.issued2012
 
dc.description.abstractIdentification and characterization of virus-host interactions are very important steps toward a better understanding of the molecular mechanisms responsible for disease progression and pathogenesis. To date, very few cellular factors involved in the life cycle of flaviviruses, which are important human pathogens, have been described. In this study, we demonstrate a crucial role for class II Arf proteins (Arf4 and Arf5) in the dengue flavivirus life cycle. We show that simultaneous depletion of Arf4 and Arf5 blocks recombinant subviral particle secretion for all four dengue serotypes. Immunostaining analysis suggests that class II Arf proteins are required at an early pre-Golgi step for dengue virus secretion. Using a horseradish peroxidase protein fused to a signal peptide, we show that class II Arfs act specifically on dengue virus secretion without altering the secretion of proteins through the constitutive secretory pathway. Co-immunoprecipitation data demonstrate that the dengue prM glycoprotein interacts with class II Arf proteins but not through its C-terminal VXPX motif. Finally, experiments performed with replication-competent dengue and yellow fever viruses demonstrate that the depletion of class II Arfs inhibits virus secretion, thus confirming their implication in the virus life cycle, although data obtained with West Nile virus pointed out the differences in virus-host interactions among flaviviruses. Our findings shed new light on a molecular mechanism used by dengue viruses during the late stages of the life cycle and demonstrate a novel function for class II Arf proteins. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationJournal Of Biological Chemistry, 2012, v. 287 n. 1, p. 767-777 [How to Cite?]
DOI: http://dx.doi.org/10.1074/jbc.M111.270579
 
dc.identifier.doihttp://dx.doi.org/10.1074/jbc.M111.270579
 
dc.identifier.epage777
 
dc.identifier.hkuros201706
 
dc.identifier.isiWOS:000298682400074
Funding AgencyGrant Number
Research Fund for Control of Infectious Diseases of Hong KongRFCID 08070952
RFCID 10091312
BNP Paribas Corporate and Investment Banking
Funding Information:

This work was supported by Research Fund for Control of Infectious Diseases of Hong Kong Grants RFCID 08070952 and RFCID 10091312 and by a donation from BNP Paribas Corporate and Investment Banking.

 
dc.identifier.issn0021-9258
2013 Impact Factor: 4.600
 
dc.identifier.issue1
 
dc.identifier.pmid22105072
 
dc.identifier.scopuseid_2-s2.0-84855290006
 
dc.identifier.spage767
 
dc.identifier.urihttp://hdl.handle.net/10722/152622
 
dc.identifier.volume287
 
dc.languageeng
 
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Biological Chemistry
 
dc.relation.referencesReferences in Scopus
 
dc.titleClass II ADP-ribosylation factors are required for efficient secretion of dengue viruses
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Institut Pasteur, Paris
  3. Brunel University