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Article: Ring finger protein RNF169 antagonizes the ubiquitin-dependent signaling cascade at sites of DNA damage
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TitleRing finger protein RNF169 antagonizes the ubiquitin-dependent signaling cascade at sites of DNA damage
 
AuthorsChen, J1
Feng, W1 2
Jiang, J2
Deng, Y2
Huen, MSY1
 
Issue Date2012
 
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
 
CitationJournal of Biological Chemistry, 2012, v. 287 n. 33, p. 27715-27722 [How to Cite?]
DOI: http://dx.doi.org/10.1074/jbc.M112.373530
 
AbstractUbiquitin signals emanating from DNA double-strand breaks (DSBs) trigger the ordered assembly of DNA damage mediator and repair proteins. This highly orchestrated process is accomplished, in part, through the concerted action of the RNF8 and RNF168 E3 ligases, which have emerged as core signaling intermediates that promote DSB-associated ubiquitylation events. In this study, we report the identification of RNF169 as a negative regulator of the DNA damage signaling cascade. We found that RNF169 interacted with ubiquitin structures and relocalized to DSBs in an RNF8/RNF168-dependent manner. Moreover, ectopic expression of RNF169 attenuated ubiquitin signaling and compromised 53BP1 accumulation at DNA damage sites, suggesting that RNF169 antagonizes RNF168 functions at DSBs. Our study unveils RNF169 as a component in DNA damage signal transduction and adds to the complexity of regulatory ubiquitylation in genome stability maintenance.
 
ISSN0021-9258
2013 Impact Factor: 4.600
 
DOIhttp://dx.doi.org/10.1074/jbc.M112.373530
 
PubMed Central IDPMC3431699
 
ISI Accession Number IDWOS:000307840700045
 
DC FieldValue
dc.contributor.authorChen, J
 
dc.contributor.authorFeng, W
 
dc.contributor.authorJiang, J
 
dc.contributor.authorDeng, Y
 
dc.contributor.authorHuen, MSY
 
dc.date.accessioned2012-07-16T09:44:13Z
 
dc.date.available2012-07-16T09:44:13Z
 
dc.date.issued2012
 
dc.description.abstractUbiquitin signals emanating from DNA double-strand breaks (DSBs) trigger the ordered assembly of DNA damage mediator and repair proteins. This highly orchestrated process is accomplished, in part, through the concerted action of the RNF8 and RNF168 E3 ligases, which have emerged as core signaling intermediates that promote DSB-associated ubiquitylation events. In this study, we report the identification of RNF169 as a negative regulator of the DNA damage signaling cascade. We found that RNF169 interacted with ubiquitin structures and relocalized to DSBs in an RNF8/RNF168-dependent manner. Moreover, ectopic expression of RNF169 attenuated ubiquitin signaling and compromised 53BP1 accumulation at DNA damage sites, suggesting that RNF169 antagonizes RNF168 functions at DSBs. Our study unveils RNF169 as a component in DNA damage signal transduction and adds to the complexity of regulatory ubiquitylation in genome stability maintenance.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationJournal of Biological Chemistry, 2012, v. 287 n. 33, p. 27715-27722 [How to Cite?]
DOI: http://dx.doi.org/10.1074/jbc.M112.373530
 
dc.identifier.doihttp://dx.doi.org/10.1074/jbc.M112.373530
 
dc.identifier.epage27722
 
dc.identifier.hkuros201054
 
dc.identifier.isiWOS:000307840700045
 
dc.identifier.issn0021-9258
2013 Impact Factor: 4.600
 
dc.identifier.issue33
 
dc.identifier.pmcidPMC3431699
 
dc.identifier.pmid22733822
 
dc.identifier.scopuseid_2-s2.0-84864977706
 
dc.identifier.spage27715
 
dc.identifier.urihttp://hdl.handle.net/10722/152621
 
dc.identifier.volume287
 
dc.languageeng
 
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Biological Chemistry
 
dc.rightsJournal of Biological Chemistry. Copyright © American Society for Biochemistry and Molecular Biology, Inc.
 
dc.subject.meshDNA Breaks, Double-Stranded
 
dc.subject.meshDNA-Binding Proteins - genetics - metabolism
 
dc.subject.meshSignal Transduction
 
dc.subject.meshUbiquitin - genetics - metabolism
 
dc.subject.meshUbiquitin-Protein Ligases - genetics - metabolism
 
dc.titleRing finger protein RNF169 antagonizes the ubiquitin-dependent signaling cascade at sites of DNA damage
 
dc.typeArticle
 
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<contributor.author>Jiang, J</contributor.author>
<contributor.author>Deng, Y</contributor.author>
<contributor.author>Huen, MSY</contributor.author>
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Author Affiliations
  1. The University of Hong Kong
  2. South China Agricultural University