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Article: Identification and characterization of bocaviruses in cats and dogs reveals a novel feline bocavirus and a novel genetic group of canine bocavirus

TitleIdentification and characterization of bocaviruses in cats and dogs reveals a novel feline bocavirus and a novel genetic group of canine bocavirus
Authors
Issue Date2012
PublisherSociety for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.org
Citation
Journal of General Virology, 2012, v. 93 n. 7, p. 1573-1582 How to Cite?
AbstractWe report the identification and genome characterization of a novel bocavirus, feline bocavirus (FBoV), and novel bocaviruses closely related to canine bocavirus (CBoV) strain Con-161 in stray cats and dogs in Hong Kong, respectively. FBoV was detected by PCR in 7.2, 0.3, 1.6, 2.0 and 0.8% of faecal, nasal, urine, kidney and blood samples, respectively, from 364 cats, while CBoV was detected in 4.6, 5.1, 6.3 and 0.3% of faecal, nasal, urine and blood samples, respectively, from 351 dogs. Three FBoV genomes sequenced revealed the presence of three ORFs characteristic of bocaviruses. Phylogenetic analysis showed that FBoVs were related only distantly to other bocaviruses, forming a distinct cluster within the genus, with
Persistent Identifierhttp://hdl.handle.net/10722/152583
ISSN
2015 Impact Factor: 3.192
2015 SCImago Journal Rankings: 1.741
ISI Accession Number ID
Funding AgencyGrant Number
Research Grant CouncilHKU 770010M
University Grant Council
Committee for Research and Conference Grant, Strategic Research Theme Fund and University Development Fund, the University of Hong Kong
Shaw Foundation
Providence Foundation Limited in memory of the late Dr Lui Hac Minh
Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health
Funding Information:

We thank Director Alan Chi-Kong Wong, Siu-Fai Leung, Thomas Hon-Chung Sit and Howard Kai-Hay Wong (HKSAR AFCD) for facilitation and support; and Veterinary Officers of the AFCD Animal Management Centres for assistance and collection of samples. We are grateful to the generous support of Mrs Carol Yu, Professor Richard Yu, Mr Hui Hoy and Mr Hui Ming in the genomic sequencing platform, and Ms Eunice Lam on emerging infectious disease research. This work is partly supported by the Research Grant Council (grant HKU 770010M), University Grant Council; Committee for Research and Conference Grant, Strategic Research Theme Fund and University Development Fund, the University of Hong Kong; the Shaw Foundation; the Providence Foundation Limited in memory of the late Dr Lui Hac Minh; a donation from Ms Eunice Lam; and the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorLau, SKPen_US
dc.contributor.authorWoo, PCYen_US
dc.contributor.authorYeung, HCen_US
dc.contributor.authorTeng, JLLen_US
dc.contributor.authorWu, Yen_US
dc.contributor.authorBai, Ren_US
dc.contributor.authorFan, RYYen_US
dc.contributor.authorChan, KHen_US
dc.contributor.authorYuen, KYen_US
dc.date.accessioned2012-07-12T08:55:54Z-
dc.date.available2012-07-12T08:55:54Z-
dc.date.issued2012en_US
dc.identifier.citationJournal of General Virology, 2012, v. 93 n. 7, p. 1573-1582en_US
dc.identifier.issn0022-1317en_US
dc.identifier.urihttp://hdl.handle.net/10722/152583-
dc.description.abstractWe report the identification and genome characterization of a novel bocavirus, feline bocavirus (FBoV), and novel bocaviruses closely related to canine bocavirus (CBoV) strain Con-161 in stray cats and dogs in Hong Kong, respectively. FBoV was detected by PCR in 7.2, 0.3, 1.6, 2.0 and 0.8% of faecal, nasal, urine, kidney and blood samples, respectively, from 364 cats, while CBoV was detected in 4.6, 5.1, 6.3 and 0.3% of faecal, nasal, urine and blood samples, respectively, from 351 dogs. Three FBoV genomes sequenced revealed the presence of three ORFs characteristic of bocaviruses. Phylogenetic analysis showed that FBoVs were related only distantly to other bocaviruses, forming a distinct cluster within the genus, with </= 5.7% nucleotide identities to the genome of minute virus of canines. The four CBoV genomes sequenced shared 87.4-89.2% nucleotide identities with that of CBoV strain Con-161. In addition to the three bocavirus ORFs, they encoded an additional ORF, ORF4, immediately downstream of the ORF for non-structural protein 1 (NS1), which was not found in other bocaviruses including CBoV strain Con-161. They also possessed a putative second exon encoding the C-terminal region of NS1 and conserved RNA-splicing signals, previously described in human bocaviruses. Partial VP1/VP2 sequence analysis of 23 FBoV and 25 CBoV strains demonstrated inter-host genetic diversity, with two potential genetic groups of FBoV and a novel CBoV group, CBoV-HK, distinct from the three groups, CBoV-A to -C, found in the USA. Although the pathogenicity of FBoV and CBoV remains to be determined, their presence in different host tissues suggested wide tissue tropism.en_US
dc.languageengen_US
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.orgen_US
dc.relation.ispartofJournal of General Virologyen_US
dc.rightsJournal of General Virology. Copyright © Society for General Microbiology.-
dc.rightsThis is an author manuscript that has been accepted for publication in [Journal Title], copyright Society for General Microbiology, but has not been copy-edited, formatted or proofed. Cite this article as appearing in [Journal Title]. This version of the manuscript may not be duplicated or reproduced, other than for personal use or within the rule of 'Fair Use of Copyrighted Materials' (section 17, Title 17, US Code), without permission from the copyright owner, Society for General Microbiology. The Society for General Microbiology disclaims any responsibility or liability for errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final copy-edited, published article, which is the version of record, can be found at [Journal URL], and is freely available without a subscription.-
dc.subject.meshBocavirus - classification - genetics - isolation and purification-
dc.subject.meshCat Diseases - virology-
dc.subject.meshDog Diseases - virology-
dc.subject.meshGenome, Viral-
dc.subject.meshParvoviridae Infections - veterinary - virology-
dc.titleIdentification and characterization of bocaviruses in cats and dogs reveals a novel feline bocavirus and a novel genetic group of canine bocavirusen_US
dc.typeArticleen_US
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hken_US
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hken_US
dc.identifier.emailTeng, JLL: llteng@hku.hken_US
dc.identifier.emailFan, RYY: rfyy@hku.hk-
dc.identifier.emailChan, KH: chankh2@hkucc.hku.hk-
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hk-
dc.identifier.authorityWoo, PCY=rp00430en_US
dc.identifier.authorityTeng, JLL=rp00277en_US
dc.identifier.authorityYuen, KY=rp00366en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1099/vir.0.042531-0en_US
dc.identifier.pmid22495233-
dc.identifier.scopuseid_2-s2.0-84862699283en_US
dc.identifier.hkuros204368-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84862699283&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume93en_US
dc.identifier.issue7en_US
dc.identifier.spage1573en_US
dc.identifier.epage1582en_US
dc.identifier.isiWOS:000306348900019-
dc.publisher.placeUnited Kingdomen_US
dc.relation.projectMolecular characterization of novel animal bocaviruses-
dc.identifier.scopusauthoridYuen, KY=36078079100en_US
dc.identifier.scopusauthoridChan, KH=7406034307en_US
dc.identifier.scopusauthoridFan, RYY=15519269300en_US
dc.identifier.scopusauthoridBai, R=54896689400en_US
dc.identifier.scopusauthoridWu, Y=54898602600en_US
dc.identifier.scopusauthoridTeng, JLL=55224168400en_US
dc.identifier.scopusauthoridYeung, HC=55258063800en_US
dc.identifier.scopusauthoridWoo, PCY=7201801340en_US
dc.identifier.scopusauthoridLau, SKP=54944624200en_US

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