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Article: Changes in microRNA expression profiles in HIV-1-transfected human cells

TitleChanges in microRNA expression profiles in HIV-1-transfected human cells
Authors
Issue Date2005
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.retrovirology.com/home/
Citation
Retrovirology, 2005, v. 2 How to Cite?
AbstractMicroRNAs (miRNAs) are small RNAs of 18-25 nucleotides (nt) in length that play important roles in regulating a variety of biological processes. Recent studies suggest that cellular miRNAs may serve to control the replication of viruses in cells. If such is the case, viruses might be expected to evolve the ability to modulate the expression of cellular miRNAs. To ask if expression of HIV-1 genes changes the miRNA profiles in human cells, we employed a high throughput microarray method, termed the RNA-primed Array-based Klenow Enzyme (RAKE) assay. Here, we describe the optimization of this assay to quantify the expression of miRNAs in HIV-1 transfected human cells. We report distinct differences in miRNA profiles in mock-transfected HeLa cells versus HeLa cells transfected with an infectious HIV-1 molecular clone, pNL4-3. © 2005 Yeung et al., licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/152580
ISSN
2015 Impact Factor: 3.989
2015 SCImago Journal Rankings: 2.506
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYeung, MLen_US
dc.contributor.authorBennasser, Yen_US
dc.contributor.authorMyers, TGen_US
dc.contributor.authorJiang, Gen_US
dc.contributor.authorBenkirane, Men_US
dc.contributor.authorJeang, KTen_US
dc.date.accessioned2012-07-12T01:56:17Z-
dc.date.available2012-07-12T01:56:17Z-
dc.date.issued2005en_US
dc.identifier.citationRetrovirology, 2005, v. 2en_US
dc.identifier.issn1742-4690en_US
dc.identifier.urihttp://hdl.handle.net/10722/152580-
dc.description.abstractMicroRNAs (miRNAs) are small RNAs of 18-25 nucleotides (nt) in length that play important roles in regulating a variety of biological processes. Recent studies suggest that cellular miRNAs may serve to control the replication of viruses in cells. If such is the case, viruses might be expected to evolve the ability to modulate the expression of cellular miRNAs. To ask if expression of HIV-1 genes changes the miRNA profiles in human cells, we employed a high throughput microarray method, termed the RNA-primed Array-based Klenow Enzyme (RAKE) assay. Here, we describe the optimization of this assay to quantify the expression of miRNAs in HIV-1 transfected human cells. We report distinct differences in miRNA profiles in mock-transfected HeLa cells versus HeLa cells transfected with an infectious HIV-1 molecular clone, pNL4-3. © 2005 Yeung et al., licensee BioMed Central Ltd.en_US
dc.languageengen_US
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.retrovirology.com/home/en_US
dc.relation.ispartofRetrovirologyen_US
dc.titleChanges in microRNA expression profiles in HIV-1-transfected human cellsen_US
dc.typeArticleen_US
dc.identifier.emailYeung, ML:pmlyeung@hku.hken_US
dc.identifier.authorityYeung, ML=rp01402en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1186/1742-4690-2-81en_US
dc.identifier.pmid16381609-
dc.identifier.pmcidPMC1352379-
dc.identifier.scopuseid_2-s2.0-30344465007en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-30344465007&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume2en_US
dc.identifier.isiWOS:000236858800001-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridYeung, ML=8350940900en_US
dc.identifier.scopusauthoridBennasser, Y=8335747500en_US
dc.identifier.scopusauthoridMyers, TG=7202530198en_US
dc.identifier.scopusauthoridJiang, G=7401706516en_US
dc.identifier.scopusauthoridBenkirane, M=7004581121en_US
dc.identifier.scopusauthoridJeang, KT=7004824803en_US

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