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Conference Paper: Photodynamic therapy induced production of cytokines by latent Epstein Barr virus infected epithelial tumor cells

TitlePhotodynamic therapy induced production of cytokines by latent Epstein Barr virus infected epithelial tumor cells
Authors
KeywordsCytokines
Epstein-Barr Virus
Photodynamic Therapy
Issue Date2007
PublisherS P I E - International Society for Optical Engineering. The Journal's web site is located at http://www.spie.org/app/Publications/index.cfm?fuseaction=proceedings
Citation
Progress In Biomedical Optics And Imaging - Proceedings Of Spie, 2007, v. 6438 How to Cite?
AbstractPhotodynamic therapy (PDT) is a method to treat cancer or non-cancer diseases by activation of the light-sensitive photosensitizers. Epstein Barr virus (EBV) has been implicated in the development of certain cancers such as nasopharyngeal carcinoma and B cell lymphoma. This study aims to examine the effects of EBV infection on the production of pro-inflammatory cytokines and chemokines in cells after the photosensitizer Zn-BC-AM PDT treatment. Epithelial tumor cell lines HONE-1 and latent EBV-infected HONE-1 (EBV-HONE-1) cells were used in this study. Cells were treated with the photosensitizer Zn-BC-AM for 24 hours before light irradiation. RT-PCR and quantitative ELISA methods were used for the evaluation of mRNA expression and production of cytokines, respectively. Results show that Zn-BC-AM PDT increases the production of IL-1α and IL-1β in EBV-HONE-1. Over a 10-fold increase in the production of H-6 was observed in the culture supernatant of Zn-BC-AM PDTtreated HONE-1 cells. PDT-induced IL-6 production was observed in HONE-1 cells. EBV-HONE-1 has a higher background level of IL-8 production than the HONE-1. The production of IL-8 was suppressed in EBV-HONE-1 cells after Zn-BC-AM PDT. Our results indicate that the response of HONE-1 cells to Zn-BC-AM PDT depends on the presence of latent EBV infection. Since IL-8 is a cytokine with angiogenic activity, Zn-BC-AM PDT may exert an anti-angiogenic effect through the suppression of IL-8 production by the EBV-infected cells.
Persistent Identifierhttp://hdl.handle.net/10722/152067
ISSN
References

 

DC FieldValueLanguage
dc.contributor.authorKoon, HKen_US
dc.contributor.authorLo, KWen_US
dc.contributor.authorLung, MLen_US
dc.contributor.authorChang, CKCen_US
dc.contributor.authorWong, RNSen_US
dc.contributor.authorMak, NKen_US
dc.date.accessioned2012-06-26T06:34:48Z-
dc.date.available2012-06-26T06:34:48Z-
dc.date.issued2007en_US
dc.identifier.citationProgress In Biomedical Optics And Imaging - Proceedings Of Spie, 2007, v. 6438en_US
dc.identifier.issn1605-7422en_US
dc.identifier.urihttp://hdl.handle.net/10722/152067-
dc.description.abstractPhotodynamic therapy (PDT) is a method to treat cancer or non-cancer diseases by activation of the light-sensitive photosensitizers. Epstein Barr virus (EBV) has been implicated in the development of certain cancers such as nasopharyngeal carcinoma and B cell lymphoma. This study aims to examine the effects of EBV infection on the production of pro-inflammatory cytokines and chemokines in cells after the photosensitizer Zn-BC-AM PDT treatment. Epithelial tumor cell lines HONE-1 and latent EBV-infected HONE-1 (EBV-HONE-1) cells were used in this study. Cells were treated with the photosensitizer Zn-BC-AM for 24 hours before light irradiation. RT-PCR and quantitative ELISA methods were used for the evaluation of mRNA expression and production of cytokines, respectively. Results show that Zn-BC-AM PDT increases the production of IL-1α and IL-1β in EBV-HONE-1. Over a 10-fold increase in the production of H-6 was observed in the culture supernatant of Zn-BC-AM PDTtreated HONE-1 cells. PDT-induced IL-6 production was observed in HONE-1 cells. EBV-HONE-1 has a higher background level of IL-8 production than the HONE-1. The production of IL-8 was suppressed in EBV-HONE-1 cells after Zn-BC-AM PDT. Our results indicate that the response of HONE-1 cells to Zn-BC-AM PDT depends on the presence of latent EBV infection. Since IL-8 is a cytokine with angiogenic activity, Zn-BC-AM PDT may exert an anti-angiogenic effect through the suppression of IL-8 production by the EBV-infected cells.en_US
dc.languageengen_US
dc.publisherS P I E - International Society for Optical Engineering. The Journal's web site is located at http://www.spie.org/app/Publications/index.cfm?fuseaction=proceedingsen_US
dc.relation.ispartofProgress in Biomedical Optics and Imaging - Proceedings of SPIEen_US
dc.subjectCytokinesen_US
dc.subjectEpstein-Barr Virusen_US
dc.subjectPhotodynamic Therapyen_US
dc.titlePhotodynamic therapy induced production of cytokines by latent Epstein Barr virus infected epithelial tumor cellsen_US
dc.typeConference_Paperen_US
dc.identifier.emailLung, ML:mlilung@hku.hken_US
dc.identifier.authorityLung, ML=rp00300en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1117/12.710533en_US
dc.identifier.scopuseid_2-s2.0-34548282541en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34548282541&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume6438en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKoon, HK=12766487800en_US
dc.identifier.scopusauthoridLo, KW=7402101603en_US
dc.identifier.scopusauthoridLung, ML=7006411788en_US
dc.identifier.scopusauthoridChang, CKC=37092348300en_US
dc.identifier.scopusauthoridWong, RNS=7402126957en_US
dc.identifier.scopusauthoridMak, NK=35582657000en_US

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