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Conference Paper: Microdissection and microcloning of chromosomal alterations in human breast cancer

TitleMicrodissection and microcloning of chromosomal alterations in human breast cancer
Authors
Issue Date1995
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0167-6806
Citation
Breast Cancer Research And Treatment, 1995, v. 33 n. 2, p. 95-102 How to Cite?
AbstractThe recognition of recurring sites of chromosome changes in malignancies has greatly facilitated the identification of genes implicated in the pathogenesis of human cancers. Based especially upon recent studies [1-4], it appears increasingly likely that a subset of recurring chromosome alterations will be recognized in human breast cancer. Currently recognized chromosome changes characterizing breast carcinoma include the recognition of cytologic features of gene amplification (e.g. double minutes [dmins] and homogeneously staining regions [HSRs]) [5-8]. As these and other chromosome regions are implicated in recurring abnormalities in breast cancer, it will become increasingly important to have band-or region-specific genomic libraries and probes in order to facilitate high resolution physical mapping and ultimately to clone breast cancer related genes [9]. Toward this end an important recent development in physical mapping has been the establishment of chromosome microdissection as a rapid and reproducible approach to rapidly isolate and characterize chromosome region-specific DNA, greatly facilitating the initial steps in positional cloning of disease-related genes [10-13]. In this brief report, we will highlight the application of chromosome microdissection to the generation of region-specific probes for both fluorescent in situ hybridization (FISH) and the generation of genomic microclone libraries. Additionally, efforts using this methodology to generate a microclone library encompassing the early onset breast/ovarian cancer (BRCA1) gene will be presented.
Persistent Identifierhttp://hdl.handle.net/10722/152066
ISSN
2015 Impact Factor: 4.085
2015 SCImago Journal Rankings: 2.424
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTrent, JMen_US
dc.contributor.authorWeber, Ben_US
dc.contributor.authorGuan, XYen_US
dc.contributor.authorZhang, Jen_US
dc.contributor.authorCollins, Fen_US
dc.contributor.authorAbel, Ken_US
dc.contributor.authorDiamond, Aen_US
dc.contributor.authorMeltzer, Pen_US
dc.date.accessioned2012-06-26T06:34:47Z-
dc.date.available2012-06-26T06:34:47Z-
dc.date.issued1995en_US
dc.identifier.citationBreast Cancer Research And Treatment, 1995, v. 33 n. 2, p. 95-102en_US
dc.identifier.issn0167-6806en_US
dc.identifier.urihttp://hdl.handle.net/10722/152066-
dc.description.abstractThe recognition of recurring sites of chromosome changes in malignancies has greatly facilitated the identification of genes implicated in the pathogenesis of human cancers. Based especially upon recent studies [1-4], it appears increasingly likely that a subset of recurring chromosome alterations will be recognized in human breast cancer. Currently recognized chromosome changes characterizing breast carcinoma include the recognition of cytologic features of gene amplification (e.g. double minutes [dmins] and homogeneously staining regions [HSRs]) [5-8]. As these and other chromosome regions are implicated in recurring abnormalities in breast cancer, it will become increasingly important to have band-or region-specific genomic libraries and probes in order to facilitate high resolution physical mapping and ultimately to clone breast cancer related genes [9]. Toward this end an important recent development in physical mapping has been the establishment of chromosome microdissection as a rapid and reproducible approach to rapidly isolate and characterize chromosome region-specific DNA, greatly facilitating the initial steps in positional cloning of disease-related genes [10-13]. In this brief report, we will highlight the application of chromosome microdissection to the generation of region-specific probes for both fluorescent in situ hybridization (FISH) and the generation of genomic microclone libraries. Additionally, efforts using this methodology to generate a microclone library encompassing the early onset breast/ovarian cancer (BRCA1) gene will be presented.en_US
dc.languageengen_US
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0167-6806en_US
dc.relation.ispartofBreast Cancer Research and Treatmenten_US
dc.subject.meshBreast Neoplasms - Geneticsen_US
dc.subject.meshChromosome Aberrations - Geneticsen_US
dc.subject.meshChromosome Mappingen_US
dc.subject.meshCloning, Molecular - Methodsen_US
dc.subject.meshDna, Neoplasm - Geneticsen_US
dc.subject.meshDissection - Methodsen_US
dc.subject.meshGene Rearrangement - Geneticsen_US
dc.subject.meshGenetic Linkageen_US
dc.subject.meshHumansen_US
dc.subject.meshIn Situ Hybridization, Fluorescenceen_US
dc.subject.meshMicromanipulationen_US
dc.titleMicrodissection and microcloning of chromosomal alterations in human breast canceren_US
dc.typeConference_Paperen_US
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_US
dc.identifier.authorityGuan, XY=rp00454en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/BF00682717en_US
dc.identifier.pmid7749145-
dc.identifier.scopuseid_2-s2.0-0028873835en_US
dc.identifier.volume33en_US
dc.identifier.issue2en_US
dc.identifier.spage95en_US
dc.identifier.epage102en_US
dc.identifier.isiWOS:A1995QG61700001-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridTrent, JM=7201692482en_US
dc.identifier.scopusauthoridWeber, B=7401435414en_US
dc.identifier.scopusauthoridGuan, XY=7201463221en_US
dc.identifier.scopusauthoridZhang, J=8539085200en_US
dc.identifier.scopusauthoridCollins, F=7403031285en_US
dc.identifier.scopusauthoridAbel, K=7006502278en_US
dc.identifier.scopusauthoridDiamond, A=7102727042en_US
dc.identifier.scopusauthoridMeltzer, P=7102464641en_US

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