Article: Viral genetic sequence variations in pandemic H1N1/2009 and seasonal H3N2 influenza viruses within an individual, a household and a community

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TitleViral genetic sequence variations in pandemic H1N1/2009 and seasonal H3N2 influenza viruses within an individual, a household and a community
AuthorsPoon, LLM1
Chan, KH1
Chu, DKW1
Fung, CCY1
Cheng, CKY1
Ip, DKM1
Leung, GM1
Peiris, JSM1 2
Cowling, BJ1
KeywordsEpidemiology
Influenza virus
Pandemic H1N1
Sequence variation
Issue Date2011
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv
CitationJournal of Clinical Virology, 2011, v. 52 n. 2, p. 146-150 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jcv.2011.06.022
AbstractBackground: There are few data in the literature on viral sequence variation between host generations/successive transmission events. Relatively little is known about the sequence heterogeneity of the influenza viruses transmitted within families. Objectives: To study the molecular epidemiology of influenza virus and to determine the sequence variation within an individual, a household and a community during the first wave of influenza pandemic in 2009. Study design: A prospective study of household transmission of influenza A in Hong Kong was conducted during the pandemic in 2009. The HA and NA sequences of pandemic and seasonal influenza A viral isolates identified in this household transmission study were sequences and analyzed. Results: Our results indicated that there were multiple introductions of influenza viruses into Hong Kong. Sequence analysis of these isolates suggested that members of these family clusters acquired the infection by household transmissions. Interestingly, unlike those concluded from previous household transmission studies, we observed sequence variations between sequential samples from the same person and also within the same household. Conclusions: Family clusters of influenza A viral infection are predominantly the result of secondary transmission within a household. Our results also suggested that the intra-host viral sequence variation might be more common that than previously thought. © 2011 Elsevier B.V.
ISSN1386-6532
2011 Impact Factor: 3.969
2011 SCImago Journal Rankings: 0.364
DOIhttp://dx.doi.org/10.1016/j.jcv.2011.06.022
ISI Accession Number IDWOS:000295535500017
Funding AgencyGrant Number
University Grants Committee Hong KongAoE/M-12/06
Food and Health Bureau, the NIH (NIAID)HHS-N266200700005C
N01-AI-70005
Harvard Center for Communicable Disease Dynamics from the National Institute of General Medical SciencesU54 GM088558
Funding Information:

This study was supported by the Area of Excellence Scheme of the University Grants Committee Hong Kong (AoE/M-12/06), the Research Fund for the Control of Infectious Disease Commissioned Project from Food and Health Bureau, the NIH (NIAID contracts HHS-N266200700005C and N01-AI-70005), and the Harvard Center for Communicable Disease Dynamics from the National Institute of General Medical Sciences (grant number U54 GM088558). The funding bodies were not involved in the collection, analysis and interpretation of data, the writing of the manuscript, or the decision to submit for publication. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of General Medical Sciences or the National Institutes of Health.

PubMed Central IDPMC3175291
ReferencesReferences in Scopus
GrantsControl of Pandemic and Inter-pandemic Influenza
DC Field
Value
dc.contributor.authorPoon, LLM
dc.contributor.authorChan, KH
dc.contributor.authorChu, DKW
dc.contributor.authorFung, CCY
dc.contributor.authorCheng, CKY
dc.contributor.authorIp, DKM
dc.contributor.authorLeung, GM
dc.contributor.authorPeiris, JSM
dc.contributor.authorCowling, BJ
dc.date.accessioned2012-06-26T06:27:52Z
dc.date.available2012-06-26T06:27:52Z
dc.date.issued2011
dc.description.abstractBackground: There are few data in the literature on viral sequence variation between host generations/successive transmission events. Relatively little is known about the sequence heterogeneity of the influenza viruses transmitted within families. Objectives: To study the molecular epidemiology of influenza virus and to determine the sequence variation within an individual, a household and a community during the first wave of influenza pandemic in 2009. Study design: A prospective study of household transmission of influenza A in Hong Kong was conducted during the pandemic in 2009. The HA and NA sequences of pandemic and seasonal influenza A viral isolates identified in this household transmission study were sequences and analyzed. Results: Our results indicated that there were multiple introductions of influenza viruses into Hong Kong. Sequence analysis of these isolates suggested that members of these family clusters acquired the infection by household transmissions. Interestingly, unlike those concluded from previous household transmission studies, we observed sequence variations between sequential samples from the same person and also within the same household. Conclusions: Family clusters of influenza A viral infection are predominantly the result of secondary transmission within a household. Our results also suggested that the intra-host viral sequence variation might be more common that than previously thought. © 2011 Elsevier B.V.
dc.description.grantControl of Pandemic and Inter-pandemic Influenza
dc.description.grantcode97655
dc.description.naturelink_to_OA_fulltext
dc.identifier.citationJournal of Clinical Virology, 2011, v. 52 n. 2, p. 146-150 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jcv.2011.06.022
dc.identifier.citeulike9628374
dc.identifier.doihttp://dx.doi.org/10.1016/j.jcv.2011.06.022
dc.identifier.epage150
dc.identifier.hkuros203694
dc.identifier.isiWOS:000295535500017
Funding AgencyGrant Number
University Grants Committee Hong KongAoE/M-12/06
Food and Health Bureau, the NIH (NIAID)HHS-N266200700005C
N01-AI-70005
Harvard Center for Communicable Disease Dynamics from the National Institute of General Medical SciencesU54 GM088558
Funding Information:

This study was supported by the Area of Excellence Scheme of the University Grants Committee Hong Kong (AoE/M-12/06), the Research Fund for the Control of Infectious Disease Commissioned Project from Food and Health Bureau, the NIH (NIAID contracts HHS-N266200700005C and N01-AI-70005), and the Harvard Center for Communicable Disease Dynamics from the National Institute of General Medical Sciences (grant number U54 GM088558). The funding bodies were not involved in the collection, analysis and interpretation of data, the writing of the manuscript, or the decision to submit for publication. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of General Medical Sciences or the National Institutes of Health.

dc.identifier.issn1386-6532
2011 Impact Factor: 3.969
2011 SCImago Journal Rankings: 0.364
dc.identifier.issue2
dc.identifier.pmcidPMC3175291
dc.identifier.pmid21802983
dc.identifier.scopuseid_2-s2.0-80052714037
dc.identifier.spage146
dc.identifier.urihttp://hdl.handle.net/10722/151754
dc.identifier.volume52
dc.languageeng
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv
dc.publisher.placeNetherlands
dc.relation.ispartofJournal of Clinical Virology
dc.relation.referencesReferences in Scopus
dc.subject.meshFamily Characteristics
dc.subject.meshGenetic Variation
dc.subject.meshHemagglutinin Glycoproteins, Influenza Virus - Genetics
dc.subject.meshHong Kong - Epidemiology
dc.subject.meshHumans
dc.subject.meshInfluenza A Virus, H1n1 Subtype - Classification - Genetics - Isolation & Purification
dc.subject.meshInfluenza A Virus, H3n2 Subtype - Classification - Genetics - Isolation & Purification
dc.subject.meshInfluenza, Human - Epidemiology - Virology
dc.subject.meshMolecular Sequence Data
dc.subject.meshNeuraminidase - Genetics
dc.subject.meshPandemics
dc.subject.meshPhylogeny
dc.subject.meshProspective Studies
dc.subject.meshResidence Characteristics
dc.subject.meshSeasons
dc.subject.meshViral Proteins - Genetics
dc.subjectEpidemiology
dc.subjectInfluenza virus
dc.subjectPandemic H1N1
dc.subjectSequence variation
dc.titleViral genetic sequence variations in pandemic H1N1/2009 and seasonal H3N2 influenza viruses within an individual, a household and a community
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. HKU-Pasteur Research Centre