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Article: Viral genetic sequence variations in pandemic H1N1/2009 and seasonal H3N2 influenza viruses within an individual, a household and a community
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TitleViral genetic sequence variations in pandemic H1N1/2009 and seasonal H3N2 influenza viruses within an individual, a household and a community
 
AuthorsPoon, LLM1
Chan, KH1
Chu, DKW1
Fung, CCY1
Cheng, CKY1
Ip, DKM1
Leung, GM1
Peiris, JSM1 2
Cowling, BJ1
 
KeywordsEpidemiology
Influenza virus
Pandemic H1N1
Sequence variation
 
Issue Date2011
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv
 
CitationJournal of Clinical Virology, 2011, v. 52 n. 2, p. 146-150 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jcv.2011.06.022
 
AbstractBackground: There are few data in the literature on viral sequence variation between host generations/successive transmission events. Relatively little is known about the sequence heterogeneity of the influenza viruses transmitted within families. Objectives: To study the molecular epidemiology of influenza virus and to determine the sequence variation within an individual, a household and a community during the first wave of influenza pandemic in 2009. Study design: A prospective study of household transmission of influenza A in Hong Kong was conducted during the pandemic in 2009. The HA and NA sequences of pandemic and seasonal influenza A viral isolates identified in this household transmission study were sequences and analyzed. Results: Our results indicated that there were multiple introductions of influenza viruses into Hong Kong. Sequence analysis of these isolates suggested that members of these family clusters acquired the infection by household transmissions. Interestingly, unlike those concluded from previous household transmission studies, we observed sequence variations between sequential samples from the same person and also within the same household. Conclusions: Family clusters of influenza A viral infection are predominantly the result of secondary transmission within a household. Our results also suggested that the intra-host viral sequence variation might be more common that than previously thought. © 2011 Elsevier B.V.
 
ISSN1386-6532
2012 Impact Factor: 3.287
2012 SCImago Journal Rankings: 1.294
 
DOIhttp://dx.doi.org/10.1016/j.jcv.2011.06.022
 
PubMed Central IDPMC3175291
 
ISI Accession Number IDWOS:000295535500017
Funding AgencyGrant Number
University Grants Committee Hong KongAoE/M-12/06
Food and Health Bureau, the NIH (NIAID)HHS-N266200700005C
N01-AI-70005
Harvard Center for Communicable Disease Dynamics from the National Institute of General Medical SciencesU54 GM088558
Funding Information:

This study was supported by the Area of Excellence Scheme of the University Grants Committee Hong Kong (AoE/M-12/06), the Research Fund for the Control of Infectious Disease Commissioned Project from Food and Health Bureau, the NIH (NIAID contracts HHS-N266200700005C and N01-AI-70005), and the Harvard Center for Communicable Disease Dynamics from the National Institute of General Medical Sciences (grant number U54 GM088558). The funding bodies were not involved in the collection, analysis and interpretation of data, the writing of the manuscript, or the decision to submit for publication. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of General Medical Sciences or the National Institutes of Health.

 
ReferencesReferences in Scopus
 
GrantsControl of Pandemic and Inter-pandemic Influenza
 
DC FieldValue
dc.contributor.authorPoon, LLM
 
dc.contributor.authorChan, KH
 
dc.contributor.authorChu, DKW
 
dc.contributor.authorFung, CCY
 
dc.contributor.authorCheng, CKY
 
dc.contributor.authorIp, DKM
 
dc.contributor.authorLeung, GM
 
dc.contributor.authorPeiris, JSM
 
dc.contributor.authorCowling, BJ
 
dc.date.accessioned2012-06-26T06:27:52Z
 
dc.date.available2012-06-26T06:27:52Z
 
dc.date.issued2011
 
dc.description.abstractBackground: There are few data in the literature on viral sequence variation between host generations/successive transmission events. Relatively little is known about the sequence heterogeneity of the influenza viruses transmitted within families. Objectives: To study the molecular epidemiology of influenza virus and to determine the sequence variation within an individual, a household and a community during the first wave of influenza pandemic in 2009. Study design: A prospective study of household transmission of influenza A in Hong Kong was conducted during the pandemic in 2009. The HA and NA sequences of pandemic and seasonal influenza A viral isolates identified in this household transmission study were sequences and analyzed. Results: Our results indicated that there were multiple introductions of influenza viruses into Hong Kong. Sequence analysis of these isolates suggested that members of these family clusters acquired the infection by household transmissions. Interestingly, unlike those concluded from previous household transmission studies, we observed sequence variations between sequential samples from the same person and also within the same household. Conclusions: Family clusters of influenza A viral infection are predominantly the result of secondary transmission within a household. Our results also suggested that the intra-host viral sequence variation might be more common that than previously thought. © 2011 Elsevier B.V.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationJournal of Clinical Virology, 2011, v. 52 n. 2, p. 146-150 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jcv.2011.06.022
 
dc.identifier.citeulike9628374
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.jcv.2011.06.022
 
dc.identifier.epage150
 
dc.identifier.hkuros203694
 
dc.identifier.isiWOS:000295535500017
Funding AgencyGrant Number
University Grants Committee Hong KongAoE/M-12/06
Food and Health Bureau, the NIH (NIAID)HHS-N266200700005C
N01-AI-70005
Harvard Center for Communicable Disease Dynamics from the National Institute of General Medical SciencesU54 GM088558
Funding Information:

This study was supported by the Area of Excellence Scheme of the University Grants Committee Hong Kong (AoE/M-12/06), the Research Fund for the Control of Infectious Disease Commissioned Project from Food and Health Bureau, the NIH (NIAID contracts HHS-N266200700005C and N01-AI-70005), and the Harvard Center for Communicable Disease Dynamics from the National Institute of General Medical Sciences (grant number U54 GM088558). The funding bodies were not involved in the collection, analysis and interpretation of data, the writing of the manuscript, or the decision to submit for publication. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of General Medical Sciences or the National Institutes of Health.

 
dc.identifier.issn1386-6532
2012 Impact Factor: 3.287
2012 SCImago Journal Rankings: 1.294
 
dc.identifier.issue2
 
dc.identifier.pmcidPMC3175291
 
dc.identifier.pmid21802983
 
dc.identifier.scopuseid_2-s2.0-80052714037
 
dc.identifier.spage146
 
dc.identifier.urihttp://hdl.handle.net/10722/151754
 
dc.identifier.volume52
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofJournal of Clinical Virology
 
dc.relation.projectControl of Pandemic and Inter-pandemic Influenza
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshFamily Characteristics
 
dc.subject.meshGenetic Variation
 
dc.subject.meshHemagglutinin Glycoproteins, Influenza Virus - Genetics
 
dc.subject.meshHong Kong - Epidemiology
 
dc.subject.meshHumans
 
dc.subject.meshInfluenza A Virus, H1n1 Subtype - Classification - Genetics - Isolation & Purification
 
dc.subject.meshInfluenza A Virus, H3n2 Subtype - Classification - Genetics - Isolation & Purification
 
dc.subject.meshInfluenza, Human - Epidemiology - Virology
 
dc.subject.meshMolecular Sequence Data
 
dc.subject.meshNeuraminidase - Genetics
 
dc.subject.meshPandemics
 
dc.subject.meshPhylogeny
 
dc.subject.meshProspective Studies
 
dc.subject.meshResidence Characteristics
 
dc.subject.meshSeasons
 
dc.subject.meshViral Proteins - Genetics
 
dc.subjectEpidemiology
 
dc.subjectInfluenza virus
 
dc.subjectPandemic H1N1
 
dc.subjectSequence variation
 
dc.titleViral genetic sequence variations in pandemic H1N1/2009 and seasonal H3N2 influenza viruses within an individual, a household and a community
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. HKU-Pasteur Research Centre