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Article: Vancomycin MIC creep in MRSA isolates from 1997 to 2008 in a healthcare region in Hong Kong

TitleVancomycin MIC creep in MRSA isolates from 1997 to 2008 in a healthcare region in Hong Kong
Authors
KeywordsMethicillin-resistant Staphylococcus aureus
Molecular epidemiology
Multilocus sequence typing
Vancomycin
Issue Date2010
PublisherWB Saunders Co Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jinf
Citation
Journal Of Infection, 2010, v. 60 n. 2, p. 140-145 How to Cite?
AbstractObjectives: To assess whether vancomycin MIC creeps among blood methicillin-resistant Staphylococcus aureus (MRSA) isolates recovered from 5 hospitals in Hong Kong from 1997 to 2008. Methods: Blood cultures MRSA isolates from 1997 to 1999 (period 1), 2004 (period 2) and 2006-2008 (period 3) were retrieved. Etest method was used to determine their vancomycin MIC. The genotypic features were determined by PCR and sequencing. Results: 247 blood MRSA isolates were studied. The vancomycin MIC were 0.375, 0.5, 0.75 and 1 mg/L for 15 (6.1%), 68 (27.5%), 89 (36%) and 75 (30.4%) isolates, respectively. There was an increase in the percentage of isolates with an MIC=1mg/L from 10.4% (5/48) during period 1 to 21.6% (8/37) during period 2 and 38.3% (62/162) during period 3 (period 1 vs. period 3, P<. 0.001). Molecular typing showed that this was due to increased percentages of clonal cluster (CC) 8/SCC. mec III/IIIA (agr group I), CC45/SCC. mec IV/V (agr group IV) and other minor clones with elevated MIC over time. Conclusion: This study found vancomycin MIC creep among blood MRSA isolates over time. As elevated MIC within the susceptible range may reduce vancomycin efficacy, clinical laboratories should adopt methods with the required precision to accurately determine MICs. © 2009 The British Infection Society.
Persistent Identifierhttp://hdl.handle.net/10722/151693
ISSN
2015 Impact Factor: 4.382
2015 SCImago Journal Rankings: 2.070
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong SAR Government
UDF Project-Research Centre of Emerging Infectious Diseases
Funding Information:

The work is supported by research grants from the Research Fund for the Control of Infectious Diseases (RFCID) of the Health, Welfare and Food Bureau of the Hong Kong SAR Government and from the UDF Project-Research Centre of Emerging Infectious Diseases.

References

 

DC FieldValueLanguage
dc.contributor.authorHo, PLen_HK
dc.contributor.authorLo, PYen_HK
dc.contributor.authorChow, KHen_HK
dc.contributor.authorLau, EHYen_HK
dc.contributor.authorLai, ELen_HK
dc.contributor.authorCheng, VCCen_HK
dc.contributor.authorKao, RYen_HK
dc.date.accessioned2012-06-26T06:26:43Z-
dc.date.available2012-06-26T06:26:43Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Infection, 2010, v. 60 n. 2, p. 140-145en_HK
dc.identifier.issn0163-4453en_HK
dc.identifier.urihttp://hdl.handle.net/10722/151693-
dc.description.abstractObjectives: To assess whether vancomycin MIC creeps among blood methicillin-resistant Staphylococcus aureus (MRSA) isolates recovered from 5 hospitals in Hong Kong from 1997 to 2008. Methods: Blood cultures MRSA isolates from 1997 to 1999 (period 1), 2004 (period 2) and 2006-2008 (period 3) were retrieved. Etest method was used to determine their vancomycin MIC. The genotypic features were determined by PCR and sequencing. Results: 247 blood MRSA isolates were studied. The vancomycin MIC were 0.375, 0.5, 0.75 and 1 mg/L for 15 (6.1%), 68 (27.5%), 89 (36%) and 75 (30.4%) isolates, respectively. There was an increase in the percentage of isolates with an MIC=1mg/L from 10.4% (5/48) during period 1 to 21.6% (8/37) during period 2 and 38.3% (62/162) during period 3 (period 1 vs. period 3, P<. 0.001). Molecular typing showed that this was due to increased percentages of clonal cluster (CC) 8/SCC. mec III/IIIA (agr group I), CC45/SCC. mec IV/V (agr group IV) and other minor clones with elevated MIC over time. Conclusion: This study found vancomycin MIC creep among blood MRSA isolates over time. As elevated MIC within the susceptible range may reduce vancomycin efficacy, clinical laboratories should adopt methods with the required precision to accurately determine MICs. © 2009 The British Infection Society.en_HK
dc.languageengen_US
dc.publisherWB Saunders Co Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jinfen_HK
dc.relation.ispartofJournal of Infectionen_HK
dc.subjectMethicillin-resistant Staphylococcus aureusen_HK
dc.subjectMolecular epidemiologyen_HK
dc.subjectMultilocus sequence typingen_HK
dc.subjectVancomycinen_HK
dc.subject.meshAnti-Bacterial Agents - Pharmacologyen_US
dc.subject.meshBacteremia - Microbiologyen_US
dc.subject.meshDna, Bacterial - Chemistry - Geneticsen_US
dc.subject.meshHong Kongen_US
dc.subject.meshHospitalsen_US
dc.subject.meshHumansen_US
dc.subject.meshMethicillin-Resistant Staphylococcus Aureus - Drug Effects - Isolation & Purificationen_US
dc.subject.meshMicrobial Sensitivity Testsen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshSequence Analysis, Dnaen_US
dc.subject.meshStaphylococcal Infections - Microbiologyen_US
dc.subject.meshVancomycin - Pharmacologyen_US
dc.subject.meshVancomycin Resistanceen_US
dc.titleVancomycin MIC creep in MRSA isolates from 1997 to 2008 in a healthcare region in Hong Kongen_HK
dc.typeArticleen_HK
dc.identifier.emailHo, PL:plho@hkucc.hku.hken_HK
dc.identifier.emailChow, KH:khchowb@hku.hken_HK
dc.identifier.emailLau, EHY:ehylau@hku.hken_HK
dc.identifier.emailKao, RY:rytkao@hkucc.hku.hken_HK
dc.identifier.authorityHo, PL=rp00406en_HK
dc.identifier.authorityChow, KH=rp00370en_HK
dc.identifier.authorityLau, EHY=rp01349en_HK
dc.identifier.authorityKao, RY=rp00481en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jinf.2009.11.011en_HK
dc.identifier.pmid19961873-
dc.identifier.scopuseid_2-s2.0-75549092161en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-75549092161&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume60en_HK
dc.identifier.issue2en_HK
dc.identifier.spage140en_HK
dc.identifier.epage145en_HK
dc.identifier.isiWOS:000274282400008-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.f100013485030-
dc.identifier.scopusauthoridHo, PL=7402211363en_HK
dc.identifier.scopusauthoridLo, PY=26423725900en_HK
dc.identifier.scopusauthoridChow, KH=7202180736en_HK
dc.identifier.scopusauthoridLau, EHY=7103086074en_HK
dc.identifier.scopusauthoridLai, EL=8238477100en_HK
dc.identifier.scopusauthoridCheng, VCC=23670479400en_HK
dc.identifier.scopusauthoridKao, RY=7101675499en_HK

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