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Article: Detecting novel low-abundant transcripts in Drosophila

TitleDetecting novel low-abundant transcripts in Drosophila
Authors
KeywordsDrosophila
Est
Genome
Low Abundant
Sage
Transcript
Issue Date2005
Citation
Rna, 2005, v. 11 n. 6, p. 939-946 How to Cite?
AbstractIncreasing evidence suggests that low-abundant transcripts may play fundamental roles in biological processes. In an attempt to estimate the prevalence of low-abundant transcripts in eukaryotic genomes, we performed a transcriptome analysis in Drosophila using the SAGE technique. We collected 244,313 SAGE tags from transcripts expressed in Drosophila embryonic, larval, pupae, adult, and testicular tissue. From these SAGE tags, we identified 40,823 unique SAGE tags. Our analysis showed that 55% of the 40,823 unique SAGE tags are novel without matches in currently known Drosophila transcripts, and most of the novel SAGE tags have low copy numbers. Further analysis indicated that these novel SAGE tags represent novel low-abundant transcripts expressed from loci outside of currently annotated exons including the intergenic and intronic regions, and antisense of the currently annotated exons in the Drosophila genome. Our study reveals the presence of a significant number of novel low-abundant transcripts in Drosophila, and highlights the need to isolate these novel low-abundant transcripts for further biological studies. Copyright © 2005 RNA Society.
Persistent Identifierhttp://hdl.handle.net/10722/151156
ISSN
2015 Impact Factor: 4.344
2015 SCImago Journal Rankings: 4.559
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLee, Sen_US
dc.contributor.authorBao, Jen_US
dc.contributor.authorZhou, Gen_US
dc.contributor.authorShapiro, Jen_US
dc.contributor.authorXu, Jen_US
dc.contributor.authorRun, ZSen_US
dc.contributor.authorLu, Xen_US
dc.contributor.authorClark, Ten_US
dc.contributor.authorJohnson, Den_US
dc.contributor.authorKim, YCen_US
dc.contributor.authorWing, Cen_US
dc.contributor.authorTseng, Cen_US
dc.contributor.authorSun, Men_US
dc.contributor.authorLin, Wen_US
dc.contributor.authorWang, Jen_US
dc.contributor.authorYang, Hen_US
dc.contributor.authorWang, Jen_US
dc.contributor.authorDu, Wen_US
dc.contributor.authorWu, CIen_US
dc.contributor.authorZhang, Xen_US
dc.contributor.authorSan, MWen_US
dc.date.accessioned2012-06-26T06:17:53Z-
dc.date.available2012-06-26T06:17:53Z-
dc.date.issued2005en_US
dc.identifier.citationRna, 2005, v. 11 n. 6, p. 939-946en_US
dc.identifier.issn1355-8382en_US
dc.identifier.urihttp://hdl.handle.net/10722/151156-
dc.description.abstractIncreasing evidence suggests that low-abundant transcripts may play fundamental roles in biological processes. In an attempt to estimate the prevalence of low-abundant transcripts in eukaryotic genomes, we performed a transcriptome analysis in Drosophila using the SAGE technique. We collected 244,313 SAGE tags from transcripts expressed in Drosophila embryonic, larval, pupae, adult, and testicular tissue. From these SAGE tags, we identified 40,823 unique SAGE tags. Our analysis showed that 55% of the 40,823 unique SAGE tags are novel without matches in currently known Drosophila transcripts, and most of the novel SAGE tags have low copy numbers. Further analysis indicated that these novel SAGE tags represent novel low-abundant transcripts expressed from loci outside of currently annotated exons including the intergenic and intronic regions, and antisense of the currently annotated exons in the Drosophila genome. Our study reveals the presence of a significant number of novel low-abundant transcripts in Drosophila, and highlights the need to isolate these novel low-abundant transcripts for further biological studies. Copyright © 2005 RNA Society.en_US
dc.languageengen_US
dc.relation.ispartofRNAen_US
dc.subjectDrosophilaen_US
dc.subjectEsten_US
dc.subjectGenomeen_US
dc.subjectLow Abundanten_US
dc.subjectSageen_US
dc.subjectTranscripten_US
dc.titleDetecting novel low-abundant transcripts in Drosophilaen_US
dc.typeArticleen_US
dc.identifier.emailSun, M:minsun@hku.hken_US
dc.identifier.authoritySun, M=rp00780en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1261/rna.7239605en_US
dc.identifier.pmid15923377-
dc.identifier.scopuseid_2-s2.0-21844454212en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-21844454212&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume11en_US
dc.identifier.issue6en_US
dc.identifier.spage939en_US
dc.identifier.epage946en_US
dc.identifier.isiWOS:000229554000010-
dc.identifier.scopusauthoridLee, S=7601418915en_US
dc.identifier.scopusauthoridBao, J=35285574800en_US
dc.identifier.scopusauthoridZhou, G=7403686673en_US
dc.identifier.scopusauthoridShapiro, J=8634549600en_US
dc.identifier.scopusauthoridXu, J=25935556000en_US
dc.identifier.scopusauthoridRun, ZS=8529869900en_US
dc.identifier.scopusauthoridLu, X=7404839480en_US
dc.identifier.scopusauthoridClark, T=7403217043en_US
dc.identifier.scopusauthoridJohnson, D=26025251000en_US
dc.identifier.scopusauthoridKim, YC=36739258200en_US
dc.identifier.scopusauthoridWing, C=26421537000en_US
dc.identifier.scopusauthoridTseng, C=35300975000en_US
dc.identifier.scopusauthoridSun, M=25932315800en_US
dc.identifier.scopusauthoridLin, W=36064845300en_US
dc.identifier.scopusauthoridWang, J=24371445200en_US
dc.identifier.scopusauthoridYang, H=7406555861en_US
dc.identifier.scopusauthoridWang, J=36078145500en_US
dc.identifier.scopusauthoridDu, W=26643257100en_US
dc.identifier.scopusauthoridWu, CI=8053335200en_US
dc.identifier.scopusauthoridZhang, X=35249930300en_US
dc.identifier.scopusauthoridSan, MW=7004498158en_US
dc.identifier.citeulike5181726-

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