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Article: Genes encoding Pir51, Beclin 1, RbAp48 and aldolase b are up or down-regulated in human primary hepatocellular carcinoma

TitleGenes encoding Pir51, Beclin 1, RbAp48 and aldolase b are up or down-regulated in human primary hepatocellular carcinoma
Authors
Issue Date2004
PublisherBaishideng Publishing Group. The Journal's web site is located at http://www.wjgnet.com/1007-9327/index.htm
Citation
World Journal Of Gastroenterology, 2004, v. 10 n. 4, p. 509-513 How to Cite?
AbstractAim: To reveal new tumor markers and target genes from differentially expressed genes of primary tumor samples using cDNA microarray. Methods: The 33P labeled cDNAs were synthesized by reverse transcription of message RNA from the liver cancerous tissue and adjacent non-cancerous liver tissue from the same patient and used to hybridize to LifeGrid 1.0 cDNA microarray blot containing 8400 known and unique human cDNA gene targets, and an expression profile of genes was produced in one paired human liver tumor tissue. After a global analysis of gene expression of 8400 genes, we selected some genes to confirm the differential expression using Northern blot and RT-PCR. Results: Parallel analysis of the hybridized signals enabled us to get an expression profile of genes in which about 500 genes were differentially expressed in the paired liver tumor tissues. We identified 4 genes, the expression of three (Beclin 1, RbAp48 and Pir51) were increased and one (aldolase b) was decreased in liver tumor tissues. In addition, the expression of these genes in 6 hepatoma cell lines was also showed by RT-PCR analysis. Conclusion: cDNA microarray permits a high throughput identification of changes in gene expression. The genes encoding Beclin 1, RbAp48, Pir51 and aldolase b are first reported that may be related with hepatocarcinoma. Copyright © 2004 by The WJG Press ISSN 1007-9327.
Persistent Identifierhttp://hdl.handle.net/10722/151141
ISSN
2015 Impact Factor: 2.787
2015 SCImago Journal Rankings: 1.076
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSong, Hen_US
dc.contributor.authorXia, SLen_US
dc.contributor.authorLiao, Cen_US
dc.contributor.authorLi, YLen_US
dc.contributor.authorWang, YFen_US
dc.contributor.authorLi, TPen_US
dc.contributor.authorZhao, MJen_US
dc.date.accessioned2012-06-26T06:17:36Z-
dc.date.available2012-06-26T06:17:36Z-
dc.date.issued2004en_US
dc.identifier.citationWorld Journal Of Gastroenterology, 2004, v. 10 n. 4, p. 509-513en_US
dc.identifier.issn1007-9327en_US
dc.identifier.urihttp://hdl.handle.net/10722/151141-
dc.description.abstractAim: To reveal new tumor markers and target genes from differentially expressed genes of primary tumor samples using cDNA microarray. Methods: The 33P labeled cDNAs were synthesized by reverse transcription of message RNA from the liver cancerous tissue and adjacent non-cancerous liver tissue from the same patient and used to hybridize to LifeGrid 1.0 cDNA microarray blot containing 8400 known and unique human cDNA gene targets, and an expression profile of genes was produced in one paired human liver tumor tissue. After a global analysis of gene expression of 8400 genes, we selected some genes to confirm the differential expression using Northern blot and RT-PCR. Results: Parallel analysis of the hybridized signals enabled us to get an expression profile of genes in which about 500 genes were differentially expressed in the paired liver tumor tissues. We identified 4 genes, the expression of three (Beclin 1, RbAp48 and Pir51) were increased and one (aldolase b) was decreased in liver tumor tissues. In addition, the expression of these genes in 6 hepatoma cell lines was also showed by RT-PCR analysis. Conclusion: cDNA microarray permits a high throughput identification of changes in gene expression. The genes encoding Beclin 1, RbAp48, Pir51 and aldolase b are first reported that may be related with hepatocarcinoma. Copyright © 2004 by The WJG Press ISSN 1007-9327.en_US
dc.languageengen_US
dc.publisherBaishideng Publishing Group. The Journal's web site is located at http://www.wjgnet.com/1007-9327/index.htmen_US
dc.relation.ispartofWorld Journal of Gastroenterologyen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshApoptosis Regulatory Proteinsen_US
dc.subject.meshCarcinoma, Hepatocellular - Genetics - Physiopathologyen_US
dc.subject.meshCarrier Proteins - Geneticsen_US
dc.subject.meshDna-Binding Proteins - Geneticsen_US
dc.subject.meshDown-Regulationen_US
dc.subject.meshFructose-Bisphosphate Aldolase - Geneticsen_US
dc.subject.meshGene Expression Regulation, Neoplasticen_US
dc.subject.meshHumansen_US
dc.subject.meshLiver Neoplasms - Genetics - Physiopathologyen_US
dc.subject.meshMembrane Proteinsen_US
dc.subject.meshNuclear Proteins - Geneticsen_US
dc.subject.meshOligonucleotide Array Sequence Analysisen_US
dc.subject.meshProteins - Geneticsen_US
dc.subject.meshRetinoblastoma-Binding Protein 4en_US
dc.subject.meshUp-Regulationen_US
dc.titleGenes encoding Pir51, Beclin 1, RbAp48 and aldolase b are up or down-regulated in human primary hepatocellular carcinomaen_US
dc.typeArticleen_US
dc.identifier.emailLi, YL:yiliang@hkucc.hku.hken_US
dc.identifier.authorityLi, YL=rp01354en_US
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.3748/wjg.v10.i4.509-
dc.identifier.pmid14966907-
dc.identifier.scopuseid_2-s2.0-1542345027en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1542345027&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume10en_US
dc.identifier.issue4en_US
dc.identifier.spage509en_US
dc.identifier.epage513en_US
dc.identifier.isiWOS:000208096000009-
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridSong, H=36984932000en_US
dc.identifier.scopusauthoridXia, SL=55222603900en_US
dc.identifier.scopusauthoridLiao, C=36830536700en_US
dc.identifier.scopusauthoridLi, YL=27171876700en_US
dc.identifier.scopusauthoridWang, YF=16644265500en_US
dc.identifier.scopusauthoridLi, TP=7406375403en_US
dc.identifier.scopusauthoridZhao, MJ=7403535359en_US

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