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Article: Carbon isotope signatures of fatty acids in Geobacter metallireducens and Shewanella algae

TitleCarbon isotope signatures of fatty acids in Geobacter metallireducens and Shewanella algae
Authors
KeywordsCarbon Isotopes
Geobacter Metallireducens
Iron-Reducing Bacteria
Lipid Biomarkers
Shewanella Algae
Issue Date2003
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chemgeo
Citation
Chemical Geology, 2003, v. 195 n. 1-4, p. 17-28 How to Cite?
AbstractThe goal of this study was to examine carbon isotope fractionations associated with lipid biomarkers of different iron-reducing bacteria. Experiments were conducted using a Geobacter metallireducens strain GS-15 (grown on acetate) and a Shewanella algae strain BrY (grown on lactate). Both organisms were grown anaerobically using ferric citrate as the electron acceptor. The δ 13C of total biomass of GS-15 was -34.3‰, which was about 7.2‰ lower than the substrate acetate (δ 13C= -27.1‰). The δ 13C of total biomass of BrY was -30.0‰, which was about 7.1‰ lower than the substrate lactate (δ 13C= -22.9‰). Isotopic fractionations (ε) between fatty acid sand biomass within the same strain were consistently greater for BrY (-10.9‰ to -15.5‰) than for GS-15 (-4.5‰ to -8.6‰). The lipid biomarker 10Me16:0, which was only found in GS-15 cultures, had an ε value of -5.39 ± 0.73‰ (n =2). The differences in fractionation may reflect the carbon assimilation pathways by which the fatty acids were synthesized: The smaller fractionations in GS-15 may be associated with the tricarboxylic cycle, whereas the greater fractionations in BrY may be associated with the serine pathway. The difference in isotope fractionations between lipid biomarkers and total biomass of different iron-reducing bacteria may be used to enhance our understanding of the microbial community structure in an iron-reducing environment. © 2002 Published by Elsevier Science B.V.
Persistent Identifierhttp://hdl.handle.net/10722/151098
ISSN
2015 Impact Factor: 3.482
2015 SCImago Journal Rankings: 2.346
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, CLen_US
dc.contributor.authorLi, Yen_US
dc.contributor.authorYe, Qen_US
dc.contributor.authorFong, Jen_US
dc.contributor.authorPeacock, ADen_US
dc.contributor.authorBlunt, Een_US
dc.contributor.authorFang, Jen_US
dc.contributor.authorLovley, DRen_US
dc.contributor.authorWhite, DCen_US
dc.date.accessioned2012-06-26T06:17:04Z-
dc.date.available2012-06-26T06:17:04Z-
dc.date.issued2003en_US
dc.identifier.citationChemical Geology, 2003, v. 195 n. 1-4, p. 17-28en_US
dc.identifier.issn0009-2541en_US
dc.identifier.urihttp://hdl.handle.net/10722/151098-
dc.description.abstractThe goal of this study was to examine carbon isotope fractionations associated with lipid biomarkers of different iron-reducing bacteria. Experiments were conducted using a Geobacter metallireducens strain GS-15 (grown on acetate) and a Shewanella algae strain BrY (grown on lactate). Both organisms were grown anaerobically using ferric citrate as the electron acceptor. The δ 13C of total biomass of GS-15 was -34.3‰, which was about 7.2‰ lower than the substrate acetate (δ 13C= -27.1‰). The δ 13C of total biomass of BrY was -30.0‰, which was about 7.1‰ lower than the substrate lactate (δ 13C= -22.9‰). Isotopic fractionations (ε) between fatty acid sand biomass within the same strain were consistently greater for BrY (-10.9‰ to -15.5‰) than for GS-15 (-4.5‰ to -8.6‰). The lipid biomarker 10Me16:0, which was only found in GS-15 cultures, had an ε value of -5.39 ± 0.73‰ (n =2). The differences in fractionation may reflect the carbon assimilation pathways by which the fatty acids were synthesized: The smaller fractionations in GS-15 may be associated with the tricarboxylic cycle, whereas the greater fractionations in BrY may be associated with the serine pathway. The difference in isotope fractionations between lipid biomarkers and total biomass of different iron-reducing bacteria may be used to enhance our understanding of the microbial community structure in an iron-reducing environment. © 2002 Published by Elsevier Science B.V.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chemgeoen_US
dc.relation.ispartofChemical Geologyen_US
dc.subjectCarbon Isotopesen_US
dc.subjectGeobacter Metallireducensen_US
dc.subjectIron-Reducing Bacteriaen_US
dc.subjectLipid Biomarkersen_US
dc.subjectShewanella Algaeen_US
dc.titleCarbon isotope signatures of fatty acids in Geobacter metallireducens and Shewanella algaeen_US
dc.typeArticleen_US
dc.identifier.emailLi, Y:yiliang@hkucc.hku.hken_US
dc.identifier.authorityLi, Y=rp01354en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0009-2541(02)00386-8en_US
dc.identifier.scopuseid_2-s2.0-0037446442en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037446442&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume195en_US
dc.identifier.issue1-4en_US
dc.identifier.spage17en_US
dc.identifier.epage28en_US
dc.identifier.isiWOS:000182179700003-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridZhang, CL=35313221000en_US
dc.identifier.scopusauthoridLi, Y=27171876700en_US
dc.identifier.scopusauthoridYe, Q=55216219400en_US
dc.identifier.scopusauthoridFong, J=7102997010en_US
dc.identifier.scopusauthoridPeacock, AD=7103229369en_US
dc.identifier.scopusauthoridBlunt, E=24524664100en_US
dc.identifier.scopusauthoridFang, J=37045275900en_US
dc.identifier.scopusauthoridLovley, DR=7102639063en_US
dc.identifier.scopusauthoridWhite, DC=35955284500en_US

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