Article: Chemically-induced cancers do not originate from bone marrow-derived cells
| Title | Chemically-induced cancers do not originate from bone marrow-derived cells | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Lin, H2 Hu, L1 3 4 Chen, L1 Yu, H2 Wang, Q2 Chen, P2 Hu, XT2 Cai, XJ2 Guan, XY1 | ||||||
| Issue Date | 2012 | ||||||
| Publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action | ||||||
| Citation | PLoS One, 2012, v. 7 n. 1, article no. e30493 [How to Cite?] DOI: http://dx.doi.org/10.1371/journal.pone.0030493 | ||||||
| Abstract | BACKGROUND: The identification and characterization of cancer stem cells (CSCs) is imperative to understanding the mechanism of cancer pathogenesis. Growing evidence suggests that CSCs play critical roles in the development and progression of cancer. However, controversy exists as to whether CSCs arise from bone marrow-derived cells (BMDCs). METHODOLOGY AND PRINCIPAL FINDINGS: In the present study, n-nitrosodiethylamine (DEN) was used to induce tumor formation in female mice that received bone marrow from male mice. Tumor formation was induced in 20/26 mice, including 12 liver tumors, 6 lung tumors, 1 bladder tumor and 1 nasopharyngeal tumor. Through comparison of fluorescence in situ hybridization (FISH) results in corresponding areas from serial tumor sections stained with HandE, we determined that BMDCs were recruited to both tumor tissue and normal surrounding tissue at a very low frequency (0.2-1% in tumors and 0-0.3% in normal tissues). However, approximately 3-70% of cells in the tissues surrounding the tumor were BMDCs, and the percentage of BMDCs was highly associated with the inflammatory status of the tissue. In the present study, no evidence was found to support the existence of fusion cells formed form BMDCs and tissue-specific stem cells. CONCLUSIONS: In summary, our data suggest that although BMDCs may contribute to tumor progression, they are unlike to contribute to tumor initiation. | ||||||
| ISSN | 1932-6203 2011 Impact Factor: 4.092 2011 SCImago Journal Rankings: 0.519 | ||||||
| DOI | http://dx.doi.org/10.1371/journal.pone.0030493 | ||||||
| ISI Accession Number ID | WOS:000301639600026
Funding Information: This work was supported by Zhejiang Provincial Natural Science Foundation of China (Y2100464) and the National Key Sci-Tech Special Project of Infectious Diseases (2012ZX10002-013). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | ||||||
| PubMed Central ID | PMC3265477 | ||||||
| References | References in Scopus |
| dc.contributor.author | Lin, H | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Hu, L | ||||||
| dc.contributor.author | Chen, L | ||||||
| dc.contributor.author | Yu, H | ||||||
| dc.contributor.author | Wang, Q | ||||||
| dc.contributor.author | Chen, P | ||||||
| dc.contributor.author | Hu, XT | ||||||
| dc.contributor.author | Cai, XJ | ||||||
| dc.contributor.author | Guan, XY | ||||||
| dc.date.accessioned | 2012-06-26T06:12:10Z | ||||||
| dc.date.available | 2012-06-26T06:12:10Z | ||||||
| dc.date.issued | 2012 | ||||||
| dc.description.abstract | BACKGROUND: The identification and characterization of cancer stem cells (CSCs) is imperative to understanding the mechanism of cancer pathogenesis. Growing evidence suggests that CSCs play critical roles in the development and progression of cancer. However, controversy exists as to whether CSCs arise from bone marrow-derived cells (BMDCs). METHODOLOGY AND PRINCIPAL FINDINGS: In the present study, n-nitrosodiethylamine (DEN) was used to induce tumor formation in female mice that received bone marrow from male mice. Tumor formation was induced in 20/26 mice, including 12 liver tumors, 6 lung tumors, 1 bladder tumor and 1 nasopharyngeal tumor. Through comparison of fluorescence in situ hybridization (FISH) results in corresponding areas from serial tumor sections stained with HandE, we determined that BMDCs were recruited to both tumor tissue and normal surrounding tissue at a very low frequency (0.2-1% in tumors and 0-0.3% in normal tissues). However, approximately 3-70% of cells in the tissues surrounding the tumor were BMDCs, and the percentage of BMDCs was highly associated with the inflammatory status of the tissue. In the present study, no evidence was found to support the existence of fusion cells formed form BMDCs and tissue-specific stem cells. CONCLUSIONS: In summary, our data suggest that although BMDCs may contribute to tumor progression, they are unlike to contribute to tumor initiation. | ||||||
| dc.description.nature | published_or_final_version | ||||||
| dc.identifier.citation | PLoS One, 2012, v. 7 n. 1, article no. e30493 [How to Cite?] DOI: http://dx.doi.org/10.1371/journal.pone.0030493 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1371/journal.pone.0030493 | ||||||
| dc.identifier.hkuros | 203478 | ||||||
| dc.identifier.isi | WOS:000301639600026
Funding Information: This work was supported by Zhejiang Provincial Natural Science Foundation of China (Y2100464) and the National Key Sci-Tech Special Project of Infectious Diseases (2012ZX10002-013). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | ||||||
| dc.identifier.issn | 1932-6203 2011 Impact Factor: 4.092 2011 SCImago Journal Rankings: 0.519 | ||||||
| dc.identifier.issue | 1, article no. e30493 | ||||||
| dc.identifier.pmcid | PMC3265477 | ||||||
| dc.identifier.pmid | 22291966 | ||||||
| dc.identifier.scopus | eid_2-s2.0-84856202914 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/150847 | ||||||
| dc.identifier.volume | 7 | ||||||
| dc.language | eng | ||||||
| dc.publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action | ||||||
| dc.publisher.place | United States | ||||||
| dc.relation.ispartof | PLoS One | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.rights | Creative Commons: Attribution 3.0 Hong Kong License | ||||||
| dc.subject.mesh | Bone Marrow Cells - pathology | ||||||
| dc.subject.mesh | Carcinogens | ||||||
| dc.subject.mesh | Cell Transformation, Neoplastic - pathology | ||||||
| dc.subject.mesh | Neoplasms - chemically induced - epidemiology - pathology | ||||||
| dc.subject.mesh | Neoplastic Stem Cells - drug effects - pathology | ||||||
| dc.title | Chemically-induced cancers do not originate from bone marrow-derived cells | ||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong
- Zhejiang University
- National Engineering Research Center of Human Stem Cells
- Central South University China