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Article: Chemically-induced cancers do not originate from bone marrow-derived cells
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TitleChemically-induced cancers do not originate from bone marrow-derived cells
 
AuthorsLin, H2
Hu, L4 3 1
Chen, L1
Yu, H2
Wang, Q2
Chen, P2
Hu, XT2
Cai, XJ2
Guan, XY1
 
Issue Date2012
 
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
 
CitationPLoS One, 2012, v. 7 n. 1, article no. e30493 [How to Cite?]
DOI: http://dx.doi.org/10.1371/journal.pone.0030493
 
AbstractBACKGROUND: The identification and characterization of cancer stem cells (CSCs) is imperative to understanding the mechanism of cancer pathogenesis. Growing evidence suggests that CSCs play critical roles in the development and progression of cancer. However, controversy exists as to whether CSCs arise from bone marrow-derived cells (BMDCs). METHODOLOGY AND PRINCIPAL FINDINGS: In the present study, n-nitrosodiethylamine (DEN) was used to induce tumor formation in female mice that received bone marrow from male mice. Tumor formation was induced in 20/26 mice, including 12 liver tumors, 6 lung tumors, 1 bladder tumor and 1 nasopharyngeal tumor. Through comparison of fluorescence in situ hybridization (FISH) results in corresponding areas from serial tumor sections stained with HandE, we determined that BMDCs were recruited to both tumor tissue and normal surrounding tissue at a very low frequency (0.2-1% in tumors and 0-0.3% in normal tissues). However, approximately 3-70% of cells in the tissues surrounding the tumor were BMDCs, and the percentage of BMDCs was highly associated with the inflammatory status of the tissue. In the present study, no evidence was found to support the existence of fusion cells formed form BMDCs and tissue-specific stem cells. CONCLUSIONS: In summary, our data suggest that although BMDCs may contribute to tumor progression, they are unlike to contribute to tumor initiation.
 
ISSN1932-6203
2013 Impact Factor: 3.534
2013 SCImago Journal Rankings: 1.724
 
DOIhttp://dx.doi.org/10.1371/journal.pone.0030493
 
PubMed Central IDPMC3265477
 
ISI Accession Number IDWOS:000301639600026
Funding AgencyGrant Number
Zhejiang Provincial Natural Science Foundation of ChinaY2100464
National Key Sci-Tech Special Project of Infectious Diseases2012ZX10002-013
Funding Information:

This work was supported by Zhejiang Provincial Natural Science Foundation of China (Y2100464) and the National Key Sci-Tech Special Project of Infectious Diseases (2012ZX10002-013). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLin, H
 
dc.contributor.authorHu, L
 
dc.contributor.authorChen, L
 
dc.contributor.authorYu, H
 
dc.contributor.authorWang, Q
 
dc.contributor.authorChen, P
 
dc.contributor.authorHu, XT
 
dc.contributor.authorCai, XJ
 
dc.contributor.authorGuan, XY
 
dc.date.accessioned2012-06-26T06:12:10Z
 
dc.date.available2012-06-26T06:12:10Z
 
dc.date.issued2012
 
dc.description.abstractBACKGROUND: The identification and characterization of cancer stem cells (CSCs) is imperative to understanding the mechanism of cancer pathogenesis. Growing evidence suggests that CSCs play critical roles in the development and progression of cancer. However, controversy exists as to whether CSCs arise from bone marrow-derived cells (BMDCs). METHODOLOGY AND PRINCIPAL FINDINGS: In the present study, n-nitrosodiethylamine (DEN) was used to induce tumor formation in female mice that received bone marrow from male mice. Tumor formation was induced in 20/26 mice, including 12 liver tumors, 6 lung tumors, 1 bladder tumor and 1 nasopharyngeal tumor. Through comparison of fluorescence in situ hybridization (FISH) results in corresponding areas from serial tumor sections stained with HandE, we determined that BMDCs were recruited to both tumor tissue and normal surrounding tissue at a very low frequency (0.2-1% in tumors and 0-0.3% in normal tissues). However, approximately 3-70% of cells in the tissues surrounding the tumor were BMDCs, and the percentage of BMDCs was highly associated with the inflammatory status of the tissue. In the present study, no evidence was found to support the existence of fusion cells formed form BMDCs and tissue-specific stem cells. CONCLUSIONS: In summary, our data suggest that although BMDCs may contribute to tumor progression, they are unlike to contribute to tumor initiation.
 
dc.description.naturepublished_or_final_version
 
dc.identifier.citationPLoS One, 2012, v. 7 n. 1, article no. e30493 [How to Cite?]
DOI: http://dx.doi.org/10.1371/journal.pone.0030493
 
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0030493
 
dc.identifier.hkuros203478
 
dc.identifier.isiWOS:000301639600026
Funding AgencyGrant Number
Zhejiang Provincial Natural Science Foundation of ChinaY2100464
National Key Sci-Tech Special Project of Infectious Diseases2012ZX10002-013
Funding Information:

This work was supported by Zhejiang Provincial Natural Science Foundation of China (Y2100464) and the National Key Sci-Tech Special Project of Infectious Diseases (2012ZX10002-013). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

 
dc.identifier.issn1932-6203
2013 Impact Factor: 3.534
2013 SCImago Journal Rankings: 1.724
 
dc.identifier.issue1, article no. e30493
 
dc.identifier.pmcidPMC3265477
 
dc.identifier.pmid22291966
 
dc.identifier.scopuseid_2-s2.0-84856202914
 
dc.identifier.urihttp://hdl.handle.net/10722/150847
 
dc.identifier.volume7
 
dc.languageeng
 
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
 
dc.publisher.placeUnited States
 
dc.relation.ispartofPLoS One
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshBone Marrow Cells - pathology
 
dc.subject.meshCarcinogens
 
dc.subject.meshCell Transformation, Neoplastic - pathology
 
dc.subject.meshNeoplasms - chemically induced - epidemiology - pathology
 
dc.subject.meshNeoplastic Stem Cells - drug effects - pathology
 
dc.titleChemically-induced cancers do not originate from bone marrow-derived cells
 
dc.typeArticle
 
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<item><contributor.author>Lin, H</contributor.author>
<contributor.author>Hu, L</contributor.author>
<contributor.author>Chen, L</contributor.author>
<contributor.author>Yu, H</contributor.author>
<contributor.author>Wang, Q</contributor.author>
<contributor.author>Chen, P</contributor.author>
<contributor.author>Hu, XT</contributor.author>
<contributor.author>Cai, XJ</contributor.author>
<contributor.author>Guan, XY</contributor.author>
<date.accessioned>2012-06-26T06:12:10Z</date.accessioned>
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<description.abstract>BACKGROUND: The identification and characterization of cancer stem cells (CSCs) is imperative to understanding the mechanism of cancer pathogenesis. Growing evidence suggests that CSCs play critical roles in the development and progression of cancer. However, controversy exists as to whether CSCs arise from bone marrow-derived cells (BMDCs). METHODOLOGY AND PRINCIPAL FINDINGS: In the present study, n-nitrosodiethylamine (DEN) was used to induce tumor formation in female mice that received bone marrow from male mice. Tumor formation was induced in 20/26 mice, including 12 liver tumors, 6 lung tumors, 1 bladder tumor and 1 nasopharyngeal tumor. Through comparison of fluorescence in situ hybridization (FISH) results in corresponding areas from serial tumor sections stained with HandE, we determined that BMDCs were recruited to both tumor tissue and normal surrounding tissue at a very low frequency (0.2-1% in tumors and 0-0.3% in normal tissues). However, approximately 3-70% of cells in the tissues surrounding the tumor were BMDCs, and the percentage of BMDCs was highly associated with the inflammatory status of the tissue. In the present study, no evidence was found to support the existence of fusion cells formed form BMDCs and tissue-specific stem cells. CONCLUSIONS: In summary, our data suggest that although BMDCs may contribute to tumor progression, they are unlike to contribute to tumor initiation.</description.abstract>
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<subject.mesh>Neoplasms - chemically induced - epidemiology - pathology</subject.mesh>
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Author Affiliations
  1. The University of Hong Kong
  2. Zhejiang University
  3. National Engineering Research Center of Human Stem Cells
  4. Central South University China